GAPDH Mouse mAb - Primary antibody, specific to GAPDH, IgG1

    Application:
  • Flow Cytometry
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
  • Clone Number: B07/4G9
  • Conjugation: Unconjugated
Item Number
Ab179920
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab179920-10μl (Trial Size)
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10μl
In stock
$49.90
Ab179920-50μl
50μl
In stock
$149.90
Ab179920-100μl
100μl
In stock
$219.90
Ab179920-1ml
1ml
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$1,699.90

mAb (B07/4G9); Mouse anti Human GAPDH Antibody; WB, Flow, ELISA; Unconjugated

Basic Description

Product NameGAPDH Mouse mAb - Primary antibody, specific to GAPDH, IgG1
Synonyms38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host speciesMouse
SpecificityGAPDH
ImmunogenRecombinant full length human GAPDH
Positive ControlWB: HEK293, HeLa, Jurkat, A431, HepG2 and K562 whole cell lysates Flow: HeLa cells
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High Performance, Validated
Note20μl卖完停产,不再备货
Product Description

Application
WB: 1/2,000-1/50,000 ; Predicted molecular weight: 37 kDa
Flow: 0.016 μg/mL-0.5 μg/mL
Description
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. This GAPDH antibody can be used as a loading control antibody.

Product Properties

IsotypeIgG1
Light Chain Typekappa
Purification MethodProtein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

GAPDH Mouse mAb (Ab179920) - Western Blot
All lanes: GAPDH Antibody (Ab179920) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 35.8 kDa
Observed band size: 36.4 kDa
Exposure time: 36.9 s

GAPDH Mouse mAb (Ab179920) - Flow Cytometry
Overlay histogram showing HeLa cells stained with GAPDH Mouse mAb (Ab179920) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Associated Targets(Human)

GAPDH Tchem Glyceraldehyde-3-phosphate dehydrogenase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry

0.016 μg/mL - 0.5 μg/mL

WB

1/2,000-1/50,000 ; Predicted molecular weight: 37 kDa

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202278Certificate of AnalysisDec 28, 2023 Ab179920
ZJ23F1202277Certificate of AnalysisDec 28, 2023 Ab179920
ZJ23F1202276Certificate of AnalysisDec 28, 2023 Ab179920

Related Documents

References

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