| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab175856-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab175856-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $207.90 | |
| Ab175856-5mg | 5mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $537.90 | |
| Ab175856-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $837.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG2; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Gevokizumab (anti-IL-1b) - Primary antibody, specific to IL1B, >95%, high purity, Human IgG2 |
|---|---|
| Synonyms | Catabolin antibody | H1 antibody | IFN beta inducing factor antibody | IL 1 antibody | IL 1 beta antibody | IL-1 beta antibody | IL1 antibody | IL1 BETA antibody | IL1B antibody | IL1B_HUMAN antibody | IL1F2 antibody | Interleukin 1 beta antibody | Interl |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Host species | Human |
| Specificity | IL1B |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | NEGATIVE ALLOSTERIC MODULATOR |
| Mechanism of action | NEGATIVE ALLOSTERIC MODULATOR of Interleukin-1 beta negative allosteric modulator |
| Product Description | Gevokizumab (anti-IL-1b) is a potent anti-IL-1β antibody, negatively modulates IL-1β signaling through an allosteric mechanism. Gevokizumab (anti-IL-1b) selectively decreases the binding affinity of IL-1β for the IL-1 receptor type I (IL-1RI) signaling receptor instead of IL-1 counter-regulatory decoy receptor (IL-1 receptor type II). |
| Isotype | Human IgG2 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 26.8 kDa (Light Chain) & 51.4 kDa (Heavy Chain), under reducing conditions; 170.1 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 1129435-60-4 |
Gevokizumab (anti-IL-1b) (Ab175856) - ELISA
Immobilized Recombinant Human IL-1 beta Protein (rp147346) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC₅₀ of 56.74 ng/mL.
Gevokizumab (anti-IL-1b) (Ab175856) - SEC
The purity of Gevokizumab (anti-IL-1b) (Ab175856) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001515 | Certificate of Analysis | Nov 02, 2023 | Ab175856 |
| ZJ23F1001524 | Certificate of Analysis | Nov 02, 2023 | Ab175856 |
| 1. Church LD, McDermott MF. (2009) Canakinumab, a fully-human mAb against IL-1beta for the potential treatment of inflammatory disorders.. Curr Opin Mol Ther, 11 (1): (81-9). [PMID:19169963] [10.1021/op500134e] |
| 2. Priestle JP, Schär HP, Grütter MG. (1989) Crystallographic refinement of interleukin 1 beta at 2.0 A resolution.. Proc Natl Acad Sci USA, 86 (24): (9667-71). [PMID:2602367] [10.1021/op500134e] |
| 3. Nanduri, V B VB, Hulmes, J D JD, Pan, Y C YC, Kilian, P L PL and Stern, A S AS.. (1991) The role of arginine residues in interleukin 1 receptor binding.. Biochimica et biophysica acta, (11): [PMID:1837236] |
| 4. Mizutani, H H, Schechter, N N, Lazarus, G G, Black, R A RA and Kupper, T S TS.. (1991) Rapid and specific conversion of precursor interleukin 1 beta (IL-1 beta) to an active IL-1 species by human mast cell chymase.. The Journal of experimental medicine, (1): [PMID:1919436] |
| 5. Clore, G M GM, Wingfield, P T PT and Gronenborn, A M AM.. (1991) High-resolution three-dimensional structure of interleukin 1 beta in solution by three- and four-dimensional nuclear magnetic resonance spectroscopy.. Biochemistry, (5): [PMID:2001363] |
| 6. Finzel, B C BC and 5 more authors.. (1989) Crystal structure of recombinant human interleukin-1 beta at 2.0 A resolution.. Journal of molecular biology, (20): [PMID:2585509] |
| 7. Bensi, G G and 5 more authors.. (1987) Human interleukin-1 beta gene.. Gene, [PMID:2954882] |
| 8. March, C J CJ and 9 more authors.. (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.. Nature, [PMID:2989698] |
| 9. Priestle, J P JP, Schär, H P HP and Grütter, M G MG.. (1988) Crystal structure of the cytokine interleukin-1 beta.. The EMBO journal, [PMID:3259176] |
| 10. Zsebo, K M KM and 6 more authors.. (1988) Effects of hematopoietin-1 and interleukin 1 activities on early hematopoietic cells of the bone marrow.. Blood, [PMID:3281727] |
| 11. Clark, B D BD, Collins, K L KL, Gandy, M S MS, Webb, A C AC and Auron, P E PE.. (1986) Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene.. Nucleic acids research, (24): [PMID:3490654] |
| 12. Nishida, T T and 7 more authors.. (1987) cDNA cloning of IL-1 alpha and IL-1 beta from mRNA of U937 cell line.. Biochemical and biophysical research communications, (27): [PMID:3493774] |
| 13. Auron, P E PE and 6 more authors.. (1984) Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6083565] |
| 14. Kapur, V V, Majesky, M W MW, Li, L L LL, Black, R A RA and Musser, J M JM.. (1993) Cleavage of interleukin 1 beta (IL-1 beta) precursor to produce active IL-1 beta by a conserved extracellular cysteine protease from Streptococcus pyogenes.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:7689226] |
| 15. Amin, A R AR and 7 more authors.. (1996) A novel mechanism of action of tetracyclines: effects on nitric oxide synthases.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:8943052] |
| 16. Vigers, G P GP, Anderson, L J LJ, Caffes, P P and Brandhuber, B J BJ.. (1997) Crystal structure of the type-I interleukin-1 receptor complexed with interleukin-1beta.. Nature, (13): [PMID:9062193] |
| 17. Tominaga, K K and 7 more authors.. (2000) IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.. International immunology, [PMID:10653850] |
| 18. Nicklin, Martin J H MJ and 5 more authors.. (2002) A sequence-based map of the nine genes of the human interleukin-1 cluster.. Genomics, [PMID:11991722] |
| 19. Oringer, R J RJ and 8 more authors.. (2002) Effect of locally delivered minocycline microspheres on markers of bone resorption.. Journal of periodontology, [PMID:12211491] |
| 20. Nakahara, Hideko H and 6 more authors.. (2003) Anti-interleukin-6 receptor antibody therapy reduces vascular endothelial growth factor production in rheumatoid arthritis.. Arthritis and rheumatism, [PMID:12794819] |
| 21. Andrei, Cristina C and 5 more authors.. (2004) Phospholipases C and A2 control lysosome-mediated IL-1 beta secretion: Implications for inflammatory processes.. Proceedings of the National Academy of Sciences of the United States of America, (29): [PMID:15192144] |
| 22. Reale, Marcella M and 5 more authors.. (2005) Acetylcholinesterase inhibitors effects on oncostatin-M, interleukin-1 beta and interleukin-6 release from lymphocytes of Alzheimer's disease patients.. Experimental gerontology, [PMID:15763393] |
| 23. and Eby, George G.. (2005) Elimination of arthritis pain and inflammation for over 2 years with a single 90 min, topical 14% gallium nitrate treatment: case reports and review of actions of gallium III.. Medical hypotheses, [PMID:16122880] |
| 24. Papin, S S and 8 more authors.. (2007) The SPRY domain of Pyrin, mutated in familial Mediterranean fever patients, interacts with inflammasome components and inhibits proIL-1beta processing.. Cell death and differentiation, [PMID:17431422] |
| 25. Mishra, Bibhuti B BB and 6 more authors.. (2010) Mycobacterium tuberculosis protein ESAT-6 is a potent activator of the NLRP3/ASC inflammasome.. Cellular microbiology, [PMID:20148899] |
| 26. Hwang, Jaegyu J, Hwang, Heehong H, Lee, Ho-Won HW and Suk, Kyoungho K.. (2010) Microglia signaling as a target of donepezil.. Neuropharmacology, [PMID:20153342] |
| 27. Wang, Dongli D and 5 more authors.. (2010) Structural insights into the assembly and activation of IL-1β with its receptors.. Nature immunology, [PMID:20802483] |
| 28. Cronstein, Bruce N BN and Sunkureddi, Prashanth P.. (2013) Mechanistic aspects of inflammation and clinical management of inflammation in acute gouty arthritis.. Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, [PMID:23319019] |
| 29. Tran, Tran H TH, Pham, Jacqueline T JT, Shafeeq, Hira H, Manigault, Kendra R KR and Arya, Vibhuti V.. (2013) Role of interleukin-1 inhibitors in the management of gout.. Pharmacotherapy, [PMID:23553601] |
| 30. Jourdan, Tony T and 13 more authors.. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes.. Nature medicine, [PMID:23955712] |
| 31. Baroja-Mazo, Alberto A and 14 more authors.. (2014) The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response.. Nature immunology, [PMID:24952504] |
| 32. Zhang, Tao T and 8 more authors.. (2015) Donepezil attenuates high glucose-accelerated senescence in human umbilical vein endothelial cells through SIRT1 activation.. Cell stress & chaperones, [PMID:26194321] |
| 33. LaRock, Christopher N CN and 8 more authors.. (2016) IL-1β is an innate immune sensor of microbial proteolysis.. Science immunology, [PMID:28331908] |
| 34. Takada, Yoko K YK and 5 more authors.. (2017) Direct binding to integrins and loss of disulfide linkage in interleukin-1β (IL-1β) are involved in the agonistic action of IL-1β.. The Journal of biological chemistry, (8): [PMID:29030430] |
| 35. LaRock, Doris L; Russell, Raedeen; Johnson, Anders F; Wilde, Shyra and LaRock, Christopher N.. (2020) Group A Streptococcus Infection of the Nasopharynx Requires Proinflammatory Signaling through the Interleukin-1 Receptor.. Infection and immunity, (18): [PMID:32719155] |
| 36. Tian, X X and 6 more authors.. (2020) NLRP6-caspase 4 inflammasome activation in response to cariogenic bacterial lipoteichoic acid in human dental pulp inflammation.. International endodontic journal, (29): [PMID:33377178] |
| 37. Driscoll, P C PC, Gronenborn, A M AM, Wingfield, P T PT and Clore, G M GM.. (1990) Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy.. Biochemistry, (15): [PMID:2372550] |
| 38. Kotenko, S V SV, Bulenkov, M T MT, Veĭko, V P VP, Epishin, S M SM and Lomakin, I B IB.. (1989) [Cloning of the cDNA coding for human prointerleukin-1 alpha and prointerleukin-1 beta].. Doklady Akademii nauk SSSR, [PMID:2635664] |
