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GFAP Antibody - Primary antibody, specific to GFAP, Rabbit IgG

Features and benefits
  • Specifications & Purity: pAb; Rabbit anti Human GFAP Antibody; WB, IHC; Unconjugated
  • Short Overview:

    Rabbit polyclonal to GFAP

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
Item Number
Ab105229
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab105229-10μl (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab105229-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab105229-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$179.90
Ab105229-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$279.90
Ab105229-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

Rabbit polyclonal to GFAP

Basic Description

Product NameGFAP Antibody - Primary antibody, specific to GFAP, Rabbit IgG
Synonymswu:fb34h11 antibody|ALXDRD antibody|cb345 antibody|etID36982.3 antibody|FLJ42474 antibody|FLJ45472 antibody|GFAP antibody|GFAP_HUMAN antibody|gfapl antibody|Glial fibrillary acidic protein antibody|Intermediate filament protein antibody|wu:fk42c12 antibod
Specifications & PuritypAb; Rabbit anti Human GFAP Antibody; WB, IHC; Unconjugated
Host speciesRabbit
SpecificityGFAP
ImmunogenRecombinant GFAP expressed in E.coli (AA 256-357)
Positive ControlWB: K562, NIH/3T3, Jurkat, SK-N-SH and C6 and cell lysates; Mouse brain lysate. IHC: Human cerebrum tissue.
ConjugateUnconjugated

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified + Protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InDry ice
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

GFAP Antibody (Ab105229) - Western Blot
All lanes: GFAP Antibody (Ab105229) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 50 kDa
Observed band size: 47, 53, 58 kDa
Exposure time: 7.5 s

GFAP Antibody (Ab105229) - Western Blot
All lanes: GFAP Antibody (Ab105229) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 44, 47,58 kDa
Exposure time: 7.5 s

GFAP Antibody (Ab105229) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 30 µg/mL GFAP Antibody (Ab105229)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.

Application

ApplicationNotes
IHC

5-30 µg/mL

WB

0.01-3 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101709Certificate of AnalysisNov 16, 2023 Ab105229
ZJ23F1101708Certificate of AnalysisNov 16, 2023 Ab105229

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References

1. Duguid, J R JR, Bohmont, C W CW, Liu, N G NG and Tourtellotte, W W WW..  (1989)  Changes in brain gene expression shared by scrapie and Alzheimer disease..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2780570]
2. Kosako, H H and 6 more authors..  (1997)  Phosphorylation of glial fibrillary acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase..  The Journal of biological chemistry,    (18):   [PMID:9099667]
3. Matsuzawa, K K and 10 more authors..  (1997)  Domain-specific phosphorylation of vimentin and glial fibrillary acidic protein by PKN..  Biochemical and biophysical research communications,    (29):   [PMID:9175763]
4. Isaacs, A A, Baker, M M, Wavrant-De Vrièze, F F and Hutton, M M..  (1998)  Determination of the gene structure of human GFAP and absence of coding region mutations associated with frontotemporal dementia with parkinsonism linked to chromosome 17..  Genomics,    (1):   [PMID:9693047]
5. Brenner, M M and 5 more authors..  (2001)  Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease..  Nature genetics,      [PMID:11138011]
6. Nielsen, Anders Lade AL and 5 more authors..  (2002)  A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins..  The Journal of biological chemistry,    (16):   [PMID:12058025]
7. Kawajiri, Aie A and 6 more authors..  (2003)  Functional significance of the specific sites phosphorylated in desmin at cleavage furrow: Aurora-B may phosphorylate and regulate type III intermediate filaments during cytokinesis coordinatedly with Rho-kinase..  Molecular biology of the cell,      [PMID:12686604]
8. Zody, Michael C MC and 73 more authors..  (2006)  DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage..  Nature,    (20):   [PMID:16625196]
9. Lee, Jung Mu and 8 more authors..  (2006)  A case of infantile Alexander disease accompanied by infantile spasms diagnosed by DNA analysis..  Journal of Korean medical science,      [PMID:17043438]
10. Hinttala, Reetta R and 5 more authors..  (2007)  Alexander disease with occipital predominance and a novel c.799G>C mutation in the GFAP gene..  Acta neuropathologica,      [PMID:17805552]
11. Bechtel, Stephanie S and 18 more authors..  (2007)  The full-ORF clone resource of the German cDNA Consortium..  BMC genomics,    (31):   [PMID:17974005]
12. Ye Wu and 5 more authors..  (2008)  Clinical and genetic study in Chinese patients with Alexander disease..  Journal of child neurology,      [PMID:18079314]
13. Kaneko, Hiroyuki H and 10 more authors..  (2009)  Novel GFAP mutation in patient with adult-onset Alexander disease presenting with spastic ataxia..  Movement disorders : official journal of the Movement Disorder Society,    (15):   [PMID:19412928]
14. Namekawa, Michito M and 5 more authors..  (2010)  Adult-onset Alexander disease with typical "tadpole" brainstem atrophy and unusual bilateral basal ganglia involvement: a case report and review of the literature..  BMC neurology,    (1):   [PMID:20359319]
15. Jin, Zhicheng and 5 more authors..  (2013)  Identification and characterization of citrulline-modified brain proteins by combining HCD and CID fragmentation..  Proteomics,      [PMID:23828821]

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