| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab105229-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab105229-50μl | 50μl | In stock | $179.90 | |
| Ab105229-100μl | 100μl | In stock | $279.90 | |
| Ab105229-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
pAb; Rabbit anti Human GFAP Antibody; WB, IHC; Unconjugated
| Product Name | GFAP Antibody - Primary antibody, specific to GFAP, Rabbit IgG |
|---|---|
| Synonyms | wu:fb34h11 antibody | ALXDRD antibody | cb345 antibody | etID36982.3 antibody | FLJ42474 antibody | FLJ45472 antibody | GFAP antibody | GFAP_HUMAN antibody | gfapl antibody | Glial fibrillary acidic protein antibody | Intermediate filament protein antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | GFAP |
| Immunogen | Recombinant GFAP expressed in E.coli (AA 256-357) |
| Positive Control | WB: K562, NIH/3T3, Jurkat, SK-N-SH and C6 and cell lysates; Mouse brain lysate. IHC: Human cerebrum tissue. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Rabbit anti Human GFAP Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-3 µg/mL IHC: 5-30 µg/mL Protein Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
GFAP Antibody (Ab105229) - Western Blot
All lanes: GFAP Antibody (Ab105229) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 50 kDa
Observed band size: 47, 53, 58 kDa
Exposure time: 7.5 s
GFAP Antibody (Ab105229) - Western Blot
All lanes: GFAP Antibody (Ab105229) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 44, 47,58 kDa
Exposure time: 7.5 s
GFAP Antibody (Ab105229) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 30 µg/mL GFAP Antibody (Ab105229)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.01-3 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101709 | Certificate of Analysis | Nov 16, 2023 | Ab105229 |
| ZJ23F1101708 | Certificate of Analysis | Nov 16, 2023 | Ab105229 |
| 1. Duguid, J R JR, Bohmont, C W CW, Liu, N G NG and Tourtellotte, W W WW.. (1989) Changes in brain gene expression shared by scrapie and Alzheimer disease.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2780570] |
| 2. Kosako, H H and 6 more authors.. (1997) Phosphorylation of glial fibrillary acidic protein at the same sites by cleavage furrow kinase and Rho-associated kinase.. The Journal of biological chemistry, (18): [PMID:9099667] |
| 3. Matsuzawa, K K and 10 more authors.. (1997) Domain-specific phosphorylation of vimentin and glial fibrillary acidic protein by PKN.. Biochemical and biophysical research communications, (29): [PMID:9175763] |
| 4. Isaacs, A A, Baker, M M, Wavrant-De Vrièze, F F and Hutton, M M.. (1998) Determination of the gene structure of human GFAP and absence of coding region mutations associated with frontotemporal dementia with parkinsonism linked to chromosome 17.. Genomics, (1): [PMID:9693047] |
| 5. Brenner, M M and 5 more authors.. (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.. Nature genetics, [PMID:11138011] |
| 6. Nielsen, Anders Lade AL and 5 more authors.. (2002) A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins.. The Journal of biological chemistry, (16): [PMID:12058025] |
| 7. Kawajiri, Aie A and 6 more authors.. (2003) Functional significance of the specific sites phosphorylated in desmin at cleavage furrow: Aurora-B may phosphorylate and regulate type III intermediate filaments during cytokinesis coordinatedly with Rho-kinase.. Molecular biology of the cell, [PMID:12686604] |
| 8. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 9. Lee, Jung Mu and 8 more authors.. (2006) A case of infantile Alexander disease accompanied by infantile spasms diagnosed by DNA analysis.. Journal of Korean medical science, [PMID:17043438] |
| 10. Hinttala, Reetta R and 5 more authors.. (2007) Alexander disease with occipital predominance and a novel c.799G>C mutation in the GFAP gene.. Acta neuropathologica, [PMID:17805552] |
| 11. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 12. Ye Wu and 5 more authors.. (2008) Clinical and genetic study in Chinese patients with Alexander disease.. Journal of child neurology, [PMID:18079314] |
| 13. Kaneko, Hiroyuki H and 10 more authors.. (2009) Novel GFAP mutation in patient with adult-onset Alexander disease presenting with spastic ataxia.. Movement disorders : official journal of the Movement Disorder Society, (15): [PMID:19412928] |
| 14. Namekawa, Michito M and 5 more authors.. (2010) Adult-onset Alexander disease with typical "tadpole" brainstem atrophy and unusual bilateral basal ganglia involvement: a case report and review of the literature.. BMC neurology, (1): [PMID:20359319] |
| 15. Jin, Zhicheng and 5 more authors.. (2013) Identification and characterization of citrulline-modified brain proteins by combining HCD and CID fragmentation.. Proteomics, [PMID:23828821] |
