Recombinant GSDMB expressed in E.coli (AA 208-237).
Positive Control
WB: A431 and MDA-MB-231 cell lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human GSDMB Antibody, Polyclonal, could be used for WB and so on. Application WB: 1/1000 Protein Function The N-terminal moiety promotes pyroptosis. May be acting by homooligomerizing within the membrane and forming pores (PubMed:27281216). The physiological relevance of this observation is unknown.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified + Peptide affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
GSDMB Antibody (Ab106583) - Western Blot All lanes: GSDMB Antibody (Ab106583) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 47 kDa Observed band size: 41, 43, 45 kDa Exposure time: 10.6 s
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2.Wan, Dafang D and 24 more authors.. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression.. Proceedings of the National Academy of Sciences of the United States of America, (2):[PMID:15498874]
3.Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20):[PMID:16625196]
4.Sin, H-S HS and 9 more authors.. (2006) Transcriptional control of the HERV-H LTR element of the GSDML gene in human tissues and cancer cells.. Archives of virology, [PMID:16625320]
5.Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13):[PMID:16959974]
6.Tamura, Masaru M and 8 more authors.. (2007) Members of a novel gene family, Gsdm, are expressed exclusively in the epithelium of the skin and gastrointestinal tract in a highly tissue-specific manner.. Genomics, [PMID:17350798]
7.Nguyen, Su Tien ST and 7 more authors.. (2007) Identification of a predictive gene expression signature of cervical lymph node metastasis in oral squamous cell carcinoma.. Cancer science, [PMID:17391312]
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9.Carl-McGrath, Stacy S, Schneider-Stock, Regine R, Ebert, Matthias M and Röcken, Christoph C.. (2008) Differential expression and localisation of gasdermin-like (GSDML), a novel member of the cancer-associated GSDMDC protein family, in neoplastic and non-neoplastic gastric, hepatic, and colon tissues.. Pathology, [PMID:18038310]
10.Saeki, Norihisa N and 10 more authors.. (2009) Distinctive expression and function of four GSDM family genes (GSDMA-D) in normal and malignant upper gastrointestinal epithelium.. Genes, chromosomes & cancer, [PMID:19051310]
11.Chao, Kinlin L KL, Kulakova, Liudmila L and Herzberg, Osnat O.. (2017) Gene polymorphism linked to increased asthma and IBD risk alters gasdermin-B structure, a sulfatide and phosphoinositide binding protein.. Proceedings of the National Academy of Sciences of the United States of America, (14):[PMID:28154144]
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