| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106592-10μl | 10μl | In stock | $69.90 | |
| Ab106592-50μl | 50μl | In stock | $199.90 | |
| Ab106592-100μl | 100μl | In stock | $289.90 | |
| Ab106592-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
pAb; Rabbit anti Human GSDMD Antibody; WB; Unconjugated
| Product Name | GSDMD Antibody - Primary antibody, specific to GSDMD, Rabbit IgG |
|---|---|
| Synonyms | Gasdermin domain-containing protein 1 | 1810036L03Rik antibody | DF 5L antibody | DF5L antibody | DFNA 5L antibody | DFNA5L antibody | FKSG 10 antibody | FKSG10 antibody | FLJ12150 antibody | Gasdermin antibody | Gasdermin D antibody | Gasdermin domain co |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | GSDMD |
| Immunogen | Recombinant GSDMD expressed in E.coli (AA 232-484) |
| Positive Control | WB: A431, U2OS and NIH/3T3 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human GSDMD Antibody, Polyclonal, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
GSDMD Antibody (Ab106592) - Western Blot
All lanes: GSDMD Antibody (Ab106592) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 32, 45, 51kDa
Exposure time: 30.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505426 | Certificate of Analysis | May 11, 2024 | Ab106592 |
| ZJ24F0505425 | Certificate of Analysis | May 11, 2024 | Ab106592 |
| ZJ24F0505424 | Certificate of Analysis | May 11, 2024 | Ab106592 |
| 1. Katoh, Masuko M and Katoh, Masaru M.. (2004) Identification and characterization of human DFNA5L, mouse Dfna5l, and rat Dfna5l genes in silico.. International journal of oncology, [PMID:15289881] |
| 2. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 3. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571] |
| 4. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 5. Saeki, Norihisa N and 10 more authors.. (2009) Distinctive expression and function of four GSDM family genes (GSDMA-D) in normal and malignant upper gastrointestinal epithelium.. Genes, chromosomes & cancer, [PMID:19051310] |
| 6. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 7. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 8. Shi, Jianjin J and 9 more authors.. (2015) Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.. Nature, (29): [PMID:26375003] |
| 9. Sborgi, Lorenzo L and 9 more authors.. (2016) GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death.. The EMBO journal, (15): [PMID:27418190] |
| 10. Rogers, Corey C and 5 more authors.. (2017) Cleavage of DFNA5 by caspase-3 during apoptosis mediates progression to secondary necrotic/pyroptotic cell death.. Nature communications, (3): [PMID:28045099] |
| 11. Lei, Xiaobo X and 5 more authors.. (2017) Enterovirus 71 Inhibits Pyroptosis through Cleavage of Gasdermin D.. Journal of virology, (15): [PMID:28679757] |
| 12. Kuang, Siyun S and 9 more authors.. (2017) Structure insight of GSDMD reveals the basis of GSDMD autoinhibition in cell pyroptosis.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:28928145] |
| 13. Liu, Zhonghua Z and 6 more authors.. (2018) Structures of the Gasdermin D C-Terminal Domains Reveal Mechanisms of Autoinhibition.. Structure (London, England : 1993), (1): [PMID:29576317] |
| 14. Ruan, Jianbin; Xia, Shiyu; Liu, Xing; Lieberman, Judy and Wu, Hao.. (2018) Cryo-EM structure of the gasdermin A3 membrane pore.. Nature, [PMID:29695864] |
| 15. Mulvihill, Estefania E and 5 more authors.. (2018) Mechanism of membrane pore formation by human gasdermin-D.. The EMBO journal, (13): [PMID:29898893] |
| 16. Humphries, Fiachra and 16 more authors.. (2020) Succination inactivates gasdermin D and blocks pyroptosis.. Science (New York, N.Y.), (25): [PMID:32820063] |
| 17. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
