| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106705-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab106705-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab106705-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab106705-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human GTF2E2 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | GTF2E2 Antibody |
|---|---|
| Synonyms | FE antibody | General transcription factor IIE subunit 2 antibody | General transcription factor IIE, polypeptide 2, beta 34kDa antibody | gtf2e2 antibody | T2EB_HUMAN antibody | TF2E 2 antibody | TF2E2 antibody | TFIIE B antibody | TFIIE-beta antibody | |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | GTF2E2 |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GTF2E2. |
| Positive Control | WB: HEK293 and HeLa cell lysates. IHC: Human heart tissue. IF/ICC: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human GTF2E2 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
GTF2E2 Antibody (Ab106705) - Western Blot
All lanes: GTF2E2 Antibody (Ab106705) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 26, 31, 32 kDa
Exposure time: 91.2 s
GTF2E2 Antibody (Ab106705) - IHC
Immunohistochemical analysis of GTF2E2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GTF2E2 Antibody (Ab106705) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
GTF2E2 Antibody (Ab106705) - IF/ICC
Immunofluorescent analysis of GTF2E2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GTF2E2 Antibody (Ab106705) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
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1/50-1/200
|
| WB |
1/500-1/1000
">
1/500-1/1000
|
| IHC | 1/50-1/100
|
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