| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106713-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab106713-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $209.90 | |
| Ab106713-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $359.90 | |
| Ab106713-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
mAb (3E8D3H8); Mouse anti Human GTF2H1 Antibody; WB, IF/ICC; Unconjugated
| Product Name | GTF2H1 Mouse mAb |
|---|---|
| Synonyms | GTF2H1 | P62 | BTF2 | TFB1 | TFIIH | Basic transcription factor 2 62 kDa subunit antibody | Basic transcription factor 62 kDa subunit antibody | BTF 2 antibody | BTF2 antibody | BTF2 p62 antibody | General transcription factor IIH polypeptide 1 antibody | |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. |
| Host species | Mouse |
| Specificity | GTF2H1 |
| Immunogen | Recombinant human GTF2H1 protein fragments expressed in E.coli. |
| Positive Control | WB: A549, Jurkat and Hep G2 cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Mouse anti Human GTF2H1 Antibody, Monoclonal (3E8D3H8), could be used for WB, IF/ICC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
GTF2H1 Mouse mAb (Ab106713) - Western Blot
All lanes: GTF2H1 Mouse mAb (Ab106713) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/5000 dilution
Predicted band size: 62 kDa
Observed band size: 66 kDa
Exposure time: 19.8 s
GTF2H1 Mouse mAb (Ab106713) - IF/ICC
Immunofluorescent analysis of GTF2H1 Mouse mAb (Ab106713) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC | 1/50-1/100 |
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