Haptoglobin Mouse mAb - Primary antibody, specific to Haptoglobin, Mouse IgG2b

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: C12
  • Conjugation: Unconjugated
Item Number
Ab106882
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab106882-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab106882-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab106882-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$359.90
Ab106882-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (C12); Mouse anti Human Haptoglobin Antibody; WB, IHC; Unconjugated

Basic Description

Product NameHaptoglobin Mouse mAb - Primary antibody, specific to Haptoglobin, Mouse IgG2b
SynonymsZonulin | Binding peptide antibody | BP antibody | Haptoglobin alpha chain antibody | Haptoglobin alpha(1S) beta antibody | Haptoglobin alpha(2FS) beta antibody | Haptoglobin beta chain antibody | Haptoglobin, alpha polypeptide antibody | Haptoglobin, bet
Specifications & PurityCarrier Free, ExactAb™, High performance, Validated, Lot by Lot
Host speciesMouse
SpecificityHaptoglobin
ImmunogenRecombinant Hpt expressed in E.coli (AA 227-378).
Positive ControlWB: Mouse liver lysates. IHC: Human liver cancer tissue.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Haptoglobin Antibody, Monoclonal (C12), could be used for WB, IHC and so on.
Application:
WB: 0.01-5 µg/mL
IHC: 5-30 µg/mL
Protein Function:
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Product Properties

IsotypeMouse IgG2b
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified + Protein G purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Haptoglobin Mouse mAb (Ab106882) - Western Blot
All lanes: Haptoglobin Mouse mAb (Ab106882) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 20.0 s

Haptoglobin Mouse mAb (Ab106882) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 30 µg/mL Haptoglobin Mouse mAb (Ab106882)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets(Human)

HP Tbio Haptoglobin (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-30 µg/mL

WB

0.01-5 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506211Certificate of AnalysisMay 28, 2024 Ab106882
ZJ24F0506210Certificate of AnalysisMay 28, 2024 Ab106882
ZJ24F0506209Certificate of AnalysisMay 28, 2024 Ab106882

Related Documents

References

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2. and Maeda, N N..  (1985)  Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene contains a retrovirus-like element..  The Journal of biological chemistry,    (10):   [PMID:2987228]
3. van der Straten, A A, Falque, J C JC, Loriau, R R, Bollen, A A and Cabezón, T T..  (1986)  Expression of cloned human haptoglobin and alpha 1-antitrypsin complementary DNAs in Saccharomyces cerevisiae..  DNA (Mary Ann Liebert, Inc.),      [PMID:3519135]
4. Bensi, G G, Raugei, G G, Klefenz, H H and Cortese, R R..  (1985)  Structure and expression of the human haptoglobin locus..  The EMBO journal,      [PMID:4018023]
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6. Raugei, G G and 6 more authors..  (1983)  Sequence of human haptoglobin cDNA: evidence that the alpha and beta subunits are coded by the same mRNA..  Nucleic acids research,    (10):   [PMID:6310515]
7. Yang, F F, Brune, J L JL, Baldwin, W D WD, Barnett, D R DR and Bowman, B H BH..  (1983)  Identification and characterization of human haptoglobin cDNA..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:6310599]
8. Maeda, N N, Yang, F F, Barnett, D R DR, Bowman, B H BH and Smithies, O O..  (1984)  Duplication within the haptoglobin Hp2 gene..  Nature,      [PMID:6325933]
9. Brune, J L JL, Yang, F F, Barnett, D R DR and Bowman, B H BH..  (1984)  Evolution of haptoglobin: comparison of complementary DNA encoding Hp alpha 1S and Hp alpha 2FS..  Nucleic acids research,    (11):   [PMID:6330675]
10. van der Straten, A A, Herzog, A A, Cabezón, T T and Bollen, A A..  (1984)  Characterization of human haptoglobin cDNAs coding for alpha 2FS beta and alpha 1S beta variants..  FEBS letters,    (12):   [PMID:6546723]
11. vander Straten, A A, Herzog, A A, Jacobs, P P, Cabezón, T T and Bollen, A A..  (1983)  Molecular cloning of human haptoglobin cDNA: evidence for a single mRNA coding for alpha 2 and beta chains..  The EMBO journal,      [PMID:6688992]
12. Kurosky, A A and 7 more authors..  (1980)  Covalent structure of human haptoglobin: a serine protease homolog..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:6997877]
13. Kliffen, M M, de Jong, P T PT and Luider, T M TM..  (1995)  Protein analysis of human maculae in relation to age-related maculopathy..  Laboratory investigation; a journal of technical methods and pathology,      [PMID:7637327]
14. Loftus, B J BJ and 18 more authors..  (1999)  Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q..  Genomics,    (15):   [PMID:10493829]
15. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R..  (2003)  Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry..  Nature biotechnology,      [PMID:12754519]
16. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR..  (2004)  Screening for N-glycosylated proteins by liquid chromatography mass spectrometry..  Proteomics,      [PMID:14760718]
17. Teye, Kwesi K and 8 more authors..  (2004)  A novel I247T missense mutation in the haptoglobin 2 beta-chain decreases the expression of the protein and is associated with ahaptoglobinemia..  Human genetics,      [PMID:14999562]
18. Martin, Joel J and 121 more authors..  (2004)  The sequence and analysis of duplication-rich human chromosome 16..  Nature,    (23):   [PMID:15616553]
19. Liu, Tao T and 6 more authors..  ()  Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry..  Journal of proteome research,      [PMID:16335952]
20. Ramachandran, Prasanna P and 5 more authors..  (2006)  Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry..  Journal of proteome research,      [PMID:16740002]
21. Levy, Andrew P AP and 19 more authors..  (2010)  Haptoglobin: basic and clinical aspects..  Antioxidants & redox signaling,      [PMID:19659435]
22. Tripathi, Amit A and 12 more authors..  (2009)  Identification of human zonulin, a physiological modulator of tight junctions, as prehaptoglobin-2..  Proceedings of the National Academy of Sciences of the United States of America,    (29):   [PMID:19805376]
23. and Fasano, Alessio A..  (2011)  Zonulin and its regulation of intestinal barrier function: the biological door to inflammation, autoimmunity, and cancer..  Physiological reviews,      [PMID:21248165]
24. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,    (7):   [PMID:22905912]
25. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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