Hck Mouse mAb - Primary antibody, specific to Hck, Mouse IgG1

    Application:
  • IF/ICC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 1508CT602.13.1
  • Conjugation: Unconjugated
Item Number
Ab106982
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab106982-10μl
10μl
In stock
$66.90
Ab106982-50μl
50μl
In stock
$199.90
Ab106982-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab106982-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,799.90

mAb (1508CT602.13.1); Mouse anti Human Hck Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameHck Mouse mAb - Primary antibody, specific to Hck, Mouse IgG1
SynonymsAIPCV | p59Hck | p61Hck | Bmk | EC 2.7.10 | EC 2.7.10.2 | Hck | Hctk | hemopoietic cell kinaseJTK9 | JTK9 | p59-HCK/p60-HCK | tyrosine-protein kinase HCK | Bmk antibody | Hck 1 antibody | Hck antibody | HCK_HUMAN antibody | Hemopoietic cell kinase antibod
Specifications & PurityExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
Host speciesMouse
SpecificityHck
ImmunogenRecombinant human HCK expressed in E.coli (AA 1-526).
Positive ControlWB: THP-1 and Hep G2 cell lysates. ICC/IF: Hep G2 cells.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Hck Antibody, Monoclonal (1508CT602.13.1), could be used for WB, ICC, IF and so on.
Application
WB: 1/2000-1/4000
ICC/IF: 1/25
Protein Function
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Hck Mouse mAb (Ab106982) - Western Blot
All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 10.0 s

Hck Mouse mAb (Ab106982) - Western Blot
All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 60 kDa
Observed band size: 58 kDa
Exposure time: 60.0 s

Hck Mouse mAb (Ab106982) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hep G2 (human liver hepatocellular carcinoma cell line) cells labeling HCK with Hck Mouse mAb (Ab106982) at 1/25 dilution,
followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Associated Targets(Human)

HCK Tclin Tyrosine-protein kinase HCK (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IF/ICC

1:25

WB

1/2000-1/4000

WB

1/2000-1/4000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303024Certificate of AnalysisMar 08, 2024 Ab106982
ZJ24F0303023Certificate of AnalysisMar 08, 2024 Ab106982

Related Documents

References

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