| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab107009-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab107009-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab107009-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $267.90 | |
| Ab107009-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,187.90 |
mAb (E22); Mouse anti Human HDAC1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | HDAC1 Mouse mAb - Primary antibody, specific to HDAC1, Mouse IgG1 |
|---|---|
| Synonyms | DKFZp686H12203 antibody | GON 10 antibody | HD1 antibody | HDAC 1 antibody | HDAC1 antibody | HDAC1_HUMAN antibody | Histone deacetylase 1 antibody | Reduced potassium dependency yeast homolog like 1 antibody | RPD3 antibody | RPD3L1 antibody | HDAC1 | GO |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | HDAC1 |
| Immunogen | Recombinant HDAC1 expressed in E.coli (AA 1-482) . |
| Positive Control | WB: A549, HeLa, HepG2, Jurkat, THP-1, HEK293 and NIH/3T3 cell lysates; IHC: Human Placenta Tissue, Human Lung cancer Tissue, Rat Colon Tissue, Rat Testis Tissue, Mouse Pancreas Tissue, Mouse Cerebellum Tissue; IF: HeLa. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human HDAC1 Antibody, Monoclonal (E22), could be used for WB, IHC, IF, ICC and so on. Application: WB: 0.2-2μg/mL IHC: 5-20μg/mL ICC/IF: 5-20μg/mL Protein Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Post-translational Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Ubiquitinated by CHFR, leading to its degradation by the proteasome. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A+G Affinity Chromatography |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
HDAC1 Mouse mAb (Ab107009) - Western Blot
All lanes: HDAC1 Mouse mAb (Ab107009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 63 kDa
Exposure time: 1.2 s
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Rat Colon Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Rat Testis Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Mouse Pancreas Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
HDAC1 Mouse mAb (Ab107009) - IHC
DAB staining on IHC
Sample: Mouse Cerebellum Tissue
Primary Ab: 10ug/mL HDAC1 Mouse mAb (Ab107009)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.2-2 µg/mL
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| IF/ICC |
5-20 µg/mL
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Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800907 | Certificate of Analysis | Aug 25, 2023 | Ab107009 |
| ZJ23F0800909 | Certificate of Analysis | Aug 25, 2023 | Ab107009 |
