mAb (A4); Mouse anti Human Heme Oxygenase 1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab107173-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab107173-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab107173-50μl | 50μl | In stock | $189.90 | |
| Ab107173-100μl | 100μl | In stock | $299.90 | |
| Ab107173-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (A4); Mouse anti Human Heme Oxygenase 1 Antibody; WB, IHC; Unconjugated
| Product Name | Heme Oxygenase 1 Mouse mAb - Primary antibody, specific to HMOX1, Mouse IgG2b |
|---|---|
| Synonyms | 32 kD antibody; BK286B10 antibody; D8Wsu38e antibody; Heat shock protein 32 kD antibody; Heat shock protein 32kD antibody; Heat shock protein antibody; Heme oxygenase (decycling) 1 antibody; Heme oxygenase 1 antibody; Hemox antibody; HMOX 1 antibody; Hmox |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | HMOX1 |
| Immunogen | Recombinant HO1 expressed in E.coli (AA 61-172) |
| Positive Control | WB: A549, HeLa, Hep G2 and HEK293 cell lysate. IHC: Human kidney tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human Heme Oxygenase 1 Antibody, Monoclonal (A4), could be used for WB, IHC and so on. Application: WB: 0.01-5µg/mL IHC: 5-30µg/mL Protein Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Heme Oxygenase 1 Mouse mAb (Ab107173) - Western Blot
All lanes: Heme Oxygenase 1 Mouse mAb (Ab107173) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 15.0 s
Heme Oxygenase 1 Mouse mAb (Ab107173) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 30 µg/mL Heme Oxygenase 1 Mouse mAb (Ab107173)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.01-5 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101690 | Certificate of Analysis | Nov 16, 2023 | Ab107173 |
| ZJ23F1101691 | Certificate of Analysis | Nov 16, 2023 | Ab107173 |
| 1. Andreev IA, Manvar D, Barreca ML, Belov DS, Basu A, Sweeney NL, Ratmanova NK, Lukyanenko ER, Manfroni G, Cecchetti V, Frick DN, Altieri A, Kaushik-Basu N, Kurkin AV.. (2015) Discovery of the 2-phenyl-4,5,6,7-Tetrahydro-1H-indole as a novel anti-hepatitis C virus targeting scaffold.. Eur J Med Chem, 96 (250-258). [PMID:25890075] |
| 2. Sorrenti V, Pittalà V, Romeo G, Amata E, Dichiara M, Marrazzo A, Turnaturi R, Prezzavento O, Barbagallo I, Vanella L, Rescifina A, Floresta G, Tibullo D, Di Raimondo F, Intagliata S, Salerno L.. (2018) Targeting heme Oxygenase-1 with hybrid compounds to overcome Imatinib resistance in chronic myeloid leukemia cell lines.. Eur J Med Chem, 158 (937-950). [PMID:30261468] |
| 3. Shibahara, S S, Sato, M M, Muller, R M RM and Yoshida, T T.. (1989) Structural organization of the human heme oxygenase gene and the function of its promoter.. European journal of biochemistry, (15): [PMID:2537723] |
| 4. Keyse, S M SM and Tyrrell, R M RM.. (1989) Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2911585] |
| 5. Yoshida, T T, Biro, P P, Cohen, T T, Müller, R M RM and Shibahara, S S.. (1988) Human heme oxygenase cDNA and induction of its mRNA by hemin.. European journal of biochemistry, (1): [PMID:3345742] |
| 6. Wilks, A A, Black, S M SM, Miller, W L WL and Ortiz de Montellano, P R PR.. (1995) Expression and characterization of truncated human heme oxygenase (hHO-1) and a fusion protein of hHO-1 with human cytochrome P450 reductase.. Biochemistry, (4): [PMID:7703255] |
| 7. Yachie, A A and 8 more authors.. (1999) Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency.. The Journal of clinical investigation, [PMID:9884342] |
| 8. Schuller, D J DJ, Wilks, A A, Ortiz de Montellano, P R PR and Poulos, T L TL.. (1999) Crystal structure of human heme oxygenase-1.. Nature structural biology, [PMID:10467099] |
| 9. Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2): [PMID:10591208] |
| 10. Lad, Latesh L and 6 more authors.. (2003) Crystal structures of the ferric, ferrous, and ferrous-NO forms of the Asp140Ala mutant of human heme oxygenase-1: catalytic implications.. Journal of molecular biology, (11): [PMID:12842469] |
| 11. Calò, Lorenzo A LA, Pagnin, Elisa E, Davis, Paul A PA, Sartori, Michelangelo M and Semplicini, Andrea A.. (2003) Oxidative stress-related factors in Bartter's and Gitelman's syndromes: relevance for angiotensin II signalling.. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, [PMID:12897089] |
| 12. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802] |
| 13. Datta, Dipak D, Banerjee, Pallavi P, Gasser, Martin M, Waaga-Gasser, Ana Maria AM and Pal, Soumitro S.. (2010) CXCR3-B can mediate growth-inhibitory signals in human renal cancer cells by down-regulating the expression of heme oxygenase-1.. The Journal of biological chemistry, (19): [PMID:20855888] |
| 14. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
