Basic Description

Product Name	HIP2/LIG Antibody - Primary antibody, specific to HIP2/LIG, Rabbit IgG
Synonyms	E2 ubiquitin-conjugating enzyme K \| EC 2.3.2.23 \| HIP-2 \| Huntingtin-interacting protein 2 \| Ubiquitin carrier protein \| Ubiquitin-conjugating enzyme E2(25K) \| Ubiquitin-conjugating enzyme E2-25 kDa \| Ubiquitin-conjugating enzyme E2-25K \| Ubiquitin-protei
Specifications & Purity	ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species	Rabbit
Specificity	HIP2/LIG
Immunogen	A KLH conjugated synthetic peptide from the N-terminal region of human UBE2K (AA 2-30).
Positive Control	WB: HeLa, HEK293, U2OS, Hep G2, Jurkat, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Rabbit anti Human HIP2/LIG Antibody, Polyclonal, could be used for WB and so on. Application WB: 1/1000 Protein Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein A purified + Peptide affinity purified
Form	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

HIP2/LIG Antibody (Ab107542) - Western Blot
All lanes: HIP2/LIG Antibody (Ab107542) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 24 kDa
Exposure time: 42.0 s

Associated Targets(Human)

UBE2K Tbio Ubiquitin-conjugating enzyme E2 K (0 Activities)

Discover Target: UBE2K

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/1000 "> 1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303353	Certificate of Analysis	Mar 19, 2024	Ab107542
ZJ24F0303352	Certificate of Analysis	Mar 19, 2024	Ab107542

FAQs and Articles

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e]
2. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211]
3. Chen H, Wu G, Gao S, Guo R, Zhao Z, Yuan H, Liu S, Wu J, Lu X, Yuan X, Yu Z, Zu X, Xie N, Yang N, Hu Z, Sun Q, Zhang W.. (2017) Discovery of Potent Small-Molecule Inhibitors of Ubiquitin-Conjugating Enzyme UbcH5c from α-Santonin Derivatives.. J Med Chem, 60 (16): (6828-6852). [PMID:28696694] [10.1021/acs.jmedchem.6b01829]
4. Kalchman, M A MA and 9 more authors.. (1996) Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.. The Journal of biological chemistry, (9): [PMID:8702625]
5. Kikuchi, J J and 7 more authors.. (2000) Induction of ubiquitin-conjugating enzyme by aggregated low density lipoprotein in human macrophages and its implications for atherosclerosis.. Arteriosclerosis, thrombosis, and vascular biology, [PMID:10634809]
6. Furukawa, Y Y and 5 more authors.. (2000) Regulation of macrophage-specific gene expression by degenerated lipoproteins.. Electrophoresis, [PMID:10675012]
7. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
8. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
9. Yamada, Misato M and 8 more authors.. (2006) NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF).. The Journal of biological chemistry, (28): [PMID:16714285]
10. Flierman, Dennis D, Coleman, Catherine S CS, Pickart, Cecile M CM, Rapoport, Tom A TA and Chau, Vincent V.. (2006) E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:16868077]
11. Christensen, Devin E DE, Brzovic, Peter S PS and Klevit, Rachel E RE.. (2007) E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages.. Nature structural & molecular biology, [PMID:17873885]
12. Wilson, Randall C RC and 5 more authors.. (2009) Structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2).. Acta crystallographica. Section F, Structural biology and crystallization communications, (1): [PMID:19407372]
13. Oh, Kwang-Jin; Kalinina, Anna and Bagchi, Srilata.. (2010) Destabilization of Rb by human papillomavirus E7 is cell cycle dependent: E2-25K is involved in the proteolysis.. Virology, (5): [PMID:19906396]
14. David, Yael Y, Ziv, Tamar T, Admon, Arie A and Navon, Ami A.. (2010) The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.. The Journal of biological chemistry, (19): [PMID:20061386]
15. Wenzel, Dawn M DM, Lissounov, Alexei A, Brzovic, Peter S PS and Klevit, Rachel E RE.. (2011) UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.. Nature, (2): [PMID:21532592]
16. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
17. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
18. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
19. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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SKU	Size	Availability	Price
Ab107542-10μl	10μl	In stock	$59.90
Ab107542-50μl	50μl	In stock	$199.90
Ab107542-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab107542-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

HIP2/LIG Antibody - Primary antibody, specific to HIP2/LIG, Rabbit IgG