HLA Class I Mouse mAb (AF700)

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2a
  • Clone Number: W6/32
  • Conjugation: Alexa Fluor® 700
Item Number
Ab191633
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab191633-10T (Trial Size)
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10T
Available within 8-12 weeks(?)
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$58.90
Ab191633-25T
25T
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Production requires sourcing of materials. We appreciate your patience and understanding.
$117.90
Ab191633-100T
100T
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Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90

mAb (W6/32); Mouse anti Human HLA Class I Antibody (AF700); Flow Cytometry

Basic Description

Product NameHLA Class I Mouse mAb (AF700)
SynonymsA-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I | HLAA | HLA-A | HLA-ABC | major histo
Specifications & PurityExactAb™, Validated, Ex:696nm, Em:719nm, 5 μL/test
Host speciesMouse
SpecificityHLA Class I
ImmunogenHuman tonsil cell membrane
Positive ControlFlow: Human peripheral blood lymphocytes
ConjugationAlexa Fluor® 700
GradeEm:719nm, Ex:696nm, ExactAb™, Validated
Product Description

Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for WB, Flow, IP, IHC, ICC/IF, Functional Assays and so on.

Product Properties

Light Chain Typekappa
Purification MethodProtein G purified
Concentration5 μL/test
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

HLA Class I Mouse mAb (AF700) (Ab191633) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with HLA Class I Mouse mAb (AF700) (Ab191633) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF700) (Ab190248).

HLA Class I Mouse mAb (AF700) (Ab191633) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with HLA Class I Mouse mAb (AF700) (Ab191633) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF700) (Ab190248). Black - Unlabelled control, cells without incubation with primary antibody.

Associated Targets(Human)

HLA-A Tclin HLA class I histocompatibility antigen, A-3 alpha chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
Flow Cytometry 5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. ">

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators