HMGA2 Antibody - Primary antibody, specific to HMGA2, Rabbit IgG

Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human HMGA2 Antibody; WB, Flow; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • Flow Cytometry
  • WB
Item Number
Ab108293
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab108293-10μl
10μl
In stock
$69.90
Ab108293-50μl
50μl
In stock
$199.90
Ab108293-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab108293-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

pAb; Rabbit anti Human HMGA2 Antibody; WB, Flow; Unconjugated

Basic Description

Product NameHMGA2 Antibody - Primary antibody, specific to HMGA2, Rabbit IgG
SynonymsHigh mobility group AT-hook protein 2 | HMGA 2 antibody | BABL antibody | High mobility group (nonhistone chromosomal) protein isoform I C antibody | High mobility group (nonhistone chromosomal) protein isoform IC antibody | High Mobility Group AT hook 2
Specifications & PurityExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host speciesRabbit
SpecificityHMGA2
ImmunogenA KLH conjugated synthetic peptide from the C-terminal region of human HMGA2 (AA 64-92).
Positive ControlWB: Hep G2, NIH/3T3 and C6 cell lysates. Flow: Hep G2 cells.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human HMGA2 Antibody, Polyclonal, could be used for WB, Flow and so on.
Application:
WB: 1/1000
Flow: 1/10-1/50
Protein Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
Post-translational
Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified + Peptide affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

HMGA2 Antibody (Ab108293) - Western Blot
All lanes: HMGA2 Antibody (Ab108293) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 16, 19 kDa
Exposure time: 120.0 s


HMGA2 Antibody (Ab108293) - Western Blot
All lanes: HMGA2 Antibody (Ab108293) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 16, 19 kDa
Exposure time: 120.0 s

HMGA2 Antibody (Ab108293) - Flow

HMGA2 Antibody (Ab108293) flow cytometric analysis of Hep G2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Associated Targets(Human)

HMGA2 Tbio High mobility group protein HMGI-C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
MFAP5 Tbio Microfibrillar-associated protein 5 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/1000

Flow Cytometry

1/10-1/50

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303357Certificate of AnalysisMar 19, 2024 Ab108293
ZJ24F0303356Certificate of AnalysisMar 19, 2024 Ab108293

Related Documents

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Chau, K Y KY, Patel, U A UA, Lee, K L KL, Lam, H Y HY and Crane-Robinson, C C..  (1995)  The gene for the human architectural transcription factor HMGI-C consists of five exons each coding for a distinct functional element..  Nucleic acids research,    (11):   [PMID:7501444]
3. Schoenmakers, E F EF and 5 more authors..  (1995)  Recurrent rearrangements in the high mobility group protein gene, HMGI-C, in benign mesenchymal tumours..  Nature genetics,      [PMID:7670494]
4. Patel, U A UA and 5 more authors..  (1994)  Expression and cDNA cloning of human HMGI-C phosphoprotein..  Biochemical and biophysical research communications,    (30):   [PMID:8198613]
5. Ashar, H R HR, Cherath, L L, Przybysz, K M KM and Chada, K K..  (1996)  Genomic characterization of human HMGIC, a member of the accessory transcription factor family found at translocation breakpoints in lipomas..  Genomics,    (15):   [PMID:8824803]
6. Petit, M M MM, Swarts, S S, Bridge, J A JA and Van de Ven, W J WJ..  (1998)  Expression of reciprocal fusion transcripts of the HMGIC and LPP genes in parosteal lipoma..  Cancer genetics and cytogenetics,    (1):   [PMID:9772904]
7. Schoenmakers, E F EF, Huysmans, C C and Van de Ven, W J WJ..  (1999)  Allelic knockout of novel splice variants of human recombination repair gene RAD51B in t(12;14) uterine leiomyomas..  Cancer research,    (1):   [PMID:9892177]
8. Rogalla, P P, Lemke, I I, Kazmierczak, B B and Bullerdiek, J J..  (2000)  An identical HMGIC-LPP fusion transcript is consistently expressed in pulmonary chondroid hamartomas with t(3;12)(q27-28;q14-15)..  Genes, chromosomes & cancer,      [PMID:11066083]
9. Quade, Bradley J BJ and 6 more authors..  (2003)  Fusion transcripts involving HMGA2 are not a common molecular mechanism in uterine leiomyomata with rearrangements in 12q15..  Cancer research,    (15):   [PMID:12649198]
10. Tessari, Michela A MA and 11 more authors..  (2003)  Transcriptional activation of the cyclin A gene by the architectural transcription factor HMGA2..  Molecular and cellular biology,      [PMID:14645522]
11. Hauke, Sven S and 6 more authors..  (2005)  Extensive expression studies revealed a complex alternative splicing pattern of the HMGA2 gene..  Biochimica et biophysica acta,    (25):   [PMID:15882911]
12. Scherer, Steven E SE and 216 more authors..  (2006)  The finished DNA sequence of human chromosome 12..  Nature,    (16):   [PMID:16541075]
13. Weedon, Michael N MN and 32 more authors..  (2007)  A common variant of HMGA2 is associated with adult and childhood height in the general population..  Nature genetics,      [PMID:17767157]
14. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
15. De Crescenzo, Agostina A and 10 more authors..  (2015)  A splicing mutation of the HMGA2 gene is associated with Silver-Russell syndrome phenotype..  Journal of human genetics,      [PMID:25809938]
16. Abi Habib, Walid W and 11 more authors..  (2018)  Genetic disruption of the oncogenic HMGA2-PLAG1-IGF2 pathway causes fetal growth restriction..  Genetics in medicine : official journal of the American College of Medical Genetics,      [PMID:28796236]
17. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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