Hoechst 33342 Staining Solution for Live Cells

Item Number

H746885

• Specifications & Purity: 100X
• Stability And Storage: Store at -20℃ in the dark for up to 1 year.
• Storage Temp: Protected from light,Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Precautions：

The concentration of this product has been optimized by to meet the needs of nuclear staining in live cells and other routine nuclear staining . If other concentrations of Hoechst 33342 are required, please purchase ’s Hoechst 33342 in powder form . Fluorescent dyes quench. Stained samples should be examined on the same day after staining.To slow down the quenching of fluorescence, we recommend mounting slides with ’s Antifade Mounting Medium .Hoechst 33342 is harmful. Please take effective measures to avoid inhalation or direct contact with skin or eyes.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Staining of live cells or tissuesa.For live cells or tissues in culture dishes, add the Hoechst 33342 Staining Solution for Live Cells (100X) to cell culture to a final concentration of 1X, and mix well gently. For example, for cells cultured in 12-well plates with 1 ml of culture per well, add 10 μl of Hoechst 33342 Staining Solution for Live Cells (100X) per well. Neither the serum nor the phenol red in culture will interfere with the staining.b.Incubate at a temperature suitable for cell growth for 10 minutes. Aspirate the culture medium containing the dye and wash 2-3 times with culture medium or PBS before observation under a fluorescence microscope. The nuclei of apoptotic cells can be densely stained, or fragmented and densely stained. If the requirement of staining effect is not particularly high, the cells can be observed after 5 minutes of staining and one washing with culture medium or PBS. To avoid removal of apoptotic cells when aspirating culture medium and washing after staining, centrifuge the cells in multi-well plates before aspiration in order to sediment the floating apoptotic cells.2.Staining of fixed cells or tissuesa.Cell or tissue samples can be stained directly with this product after fixation and washing appropriately. However, if immunofluorescence staining of the sample is required, perform immunofluorescence staining first, and then Hoechst 33342 staining. b.For adherent cells or tissue sections, add a small amount of Hoechst 33342 Staining Solution to cover the sample. For suspension cells, add the Hoechst 33342 Staining Solution at least 3 times the volume of the sample, and mix well.c.Leave the sample at room temperature for 3-5 minutes. d.Aspirate the Hoechst 33342 Staining Solution and wash with TBST, PBS or saline for 2-3 times, each time for 3-5 minutes.e.Observe under the fluorescence microscope directly or after mounting. The nuclei of apoptotic cells can be densely stained or fragmented and densely stained.Related Products:Cat. No.Product NamePack SizeC1002DAPI5mg/ml×0.2mlC1005DAPI Staining Solution 10mlC1006DAPI Staining Solution50mlC1011Hoechst 3325810mgC1017Hoechst 33258 Staining Solution10mlC1018Hoechst 33258 Staining Solution50mlC1022Hoechst 3334210mgC1025Hoechst 33342 Staining Solution10mlC1026Hoechst 33342 Staining Solution for Live Cells (100X)50mlC1027Hoechst 33342 Staining Solution for Live Cells (100X)0.1mlC1028Hoechst 33342 Staining Solution for Live Cells (100X) 0.5mlC1029Hoechst 33342 Staining Solution for Live Cells (100X)3ml