| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab108619-10μl | 10μl | In stock | $69.90 | |
| Ab108619-50μl | 50μl | In stock | $189.90 | |
| Ab108619-100μl | 100μl | In stock | $279.90 | |
| Ab108619-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
pAb; Rabbit anti Human HP1 gamma/CBX3 Antibody; WB; Unconjugated
| Product Name | HP1 gamma/CBX3 Antibody - Primary antibody, specific to CBX3, Rabbit IgG |
|---|---|
| Synonyms | HECH | Heterochromatin protein 1 homolog gamma | HP1 gamma | Modifier 2 protein | CBX 3 antibody | CBX3 antibody | CBX3_HUMAN antibody | Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody | Chromobox homolog 3 antibody | Chromobox protein homolo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | CBX3 |
| Immunogen | Recombinant CBX3 expressed in E.coli (AA 1-183). |
| Positive Control | WB: A375, HeLa, Hep G2, HUVEC, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human HP1 gamma/CBX3 Antibody, Polyclonal, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
HP1 gamma/CBX3 Antibody (Ab108619) - Western Blot
All lanes: HP1 gamma/CBX3 Antibody (Ab108619) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 22 kDa
Exposure time: 1.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303933 | Certificate of Analysis | Mar 31, 2024 | Ab108619 |
| ZJ24F0303932 | Certificate of Analysis | Mar 31, 2024 | Ab108619 |
| ZJ24F0303931 | Certificate of Analysis | Mar 31, 2024 | Ab108619 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Ren C, Smith SG, Yap K, Li S, Li J, Mezei M, Rodriguez Y, Vincek A, Aguilo F, Walsh MJ, Zhou MM.. (2016) Structure-Guided Discovery of Selective Antagonists for the Chromodomain of Polycomb Repressive Protein CBX7.. ACS Med Chem Lett, 7 (6): (601-605). [PMID:27326334] [10.1021/acsmedchemlett.6b00042] |
| 3. Stuckey JI, Simpson C, Norris-Drouin JL, Cholensky SH, Lee J, Pasca R, Cheng N, Dickson BM, Pearce KH, Frye SV, James LI.. (2016) Structure-Activity Relationships and Kinetic Studies of Peptidic Antagonists of CBX Chromodomains.. J Med Chem, 59 (19): (8913-8923). [PMID:27571219] [10.1021/acs.jmedchem.6b00801] |
| 4. Ye, Q Q and Worman, H J HJ.. (1996) Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1.. The Journal of biological chemistry, (21): [PMID:8663349] |
| 5. Ye, Q Q, Callebaut, I I, Pezhman, A A, Courvalin, J C JC and Worman, H J HJ.. (1997) Domain-specific interactions of human HP1-type chromodomain proteins and inner nuclear membrane protein LBR.. The Journal of biological chemistry, (6): [PMID:9169472] |
| 6. Seeler, J S JS, Marchio, A A, Sitterlin, D D, Transy, C C and Dejean, A A.. (1998) Interaction of SP100 with HP1 proteins: a link between the promyelocytic leukemia-associated nuclear bodies and the chromatin compartment.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:9636146] |
| 7. Ainsztein, A M AM, Kandels-Lewis, S E SE, Mackay, A M AM and Earnshaw, W C WC.. (1998) INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1.. The Journal of cell biology, (28): [PMID:9864353] |
| 8. Ryan, R F RF and 6 more authors.. (1999) KAP-1 corepressor protein interacts and colocalizes with heterochromatic and euchromatic HP1 proteins: a potential role for Krüppel-associated box-zinc finger proteins in heterochromatin-mediated gene silencing.. Molecular and cellular biology, [PMID:10330177] |
| 9. Minc, E E, Allory, Y Y, Worman, H J HJ, Courvalin, J C JC and Buendia, B B.. (1999) Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.. Chromosoma, [PMID:10460410] |
| 10. Koike, N N, Maita, H H, Taira, T T, Ariga, H H and Iguchi-Ariga, S M SM.. (2000) Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1).. FEBS letters, (4): [PMID:10664448] |
| 11. Lachner, M M, O'Carroll, D D, Rea, S S, Mechtler, K K and Jenuwein, T T.. (2001) Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.. Nature, (1): [PMID:11242053] |
| 12. Ogawa, Hidesato H, Ishiguro, Kei-Ichiro K, Gaubatz, Stefan S, Livingston, David M DM and Nakatani, Yoshihiro Y.. (2002) A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.. Science (New York, N.Y.), (10): [PMID:12004135] |
| 13. Obuse, Chikashi C and 5 more authors.. (2004) A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.. Nature cell biology, [PMID:15502821] |
| 14. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 15. Han, Guanghui G and 9 more authors.. (2008) Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography.. Proteomics, [PMID:18318008] |
| 16. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 17. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 18. Lee, Sang-Wang and 6 more authors.. (2010) ASXL1 represses retinoic acid receptor-mediated transcription through associating with HP1 and LSD1.. The Journal of biological chemistry, (1): [PMID:19880879] |
| 19. Nozawa, Ryu-Suke RS and 7 more authors.. (2010) Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation.. Nature cell biology, [PMID:20562864] |
| 20. Vermeulen, Michiel M and 10 more authors.. (2010) Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.. Cell, (17): [PMID:20850016] |
| 21. Kang, Jungseog J and 5 more authors.. (2011) Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells.. Molecular biology of the cell, (15): [PMID:21346195] |
| 22. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 24. Brants, Jan J and 11 more authors.. (2012) Tubulin tyrosine ligase like 12, a TTLL family member with SET- and TTL-like domains and roles in histone and tubulin modifications and mitosis.. PloS one, [PMID:23251473] |
| 25. Nozawa, Ryu-Suke RS and 8 more authors.. (2013) Human inactive X chromosome is compacted through a PRC2-independent SMCHD1-HBiX1 pathway.. Nature structural & molecular biology, [PMID:23542155] |
| 26. . (2015) ASXL1 represses retinoic acid receptor-mediated transcription through associating with HP1 and LSD1.. The Journal of biological chemistry, (6): [PMID:25750265] |
| 27. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 28. Bot, Christopher C and 5 more authors.. (2017) Independent mechanisms recruit the cohesin loader protein NIPBL to sites of DNA damage.. Journal of cell science, (15): [PMID:28167679] |
| 29. Hoffmeister, Helen H and 12 more authors.. (2017) CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality.. Nucleic acids research, (13): [PMID:28977666] |
| 30. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
