| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab108837-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab108837-20μl | 20μl | In stock | $99.90 | |
| Ab108837-50μl | 50μl | In stock | $189.90 | |
| Ab108837-100μl | 100μl | In stock | $299.90 | |
| Ab108837-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (D2); Mouse anti Human Hsp27 Antibody; WB; Unconjugated
| Product Name | Hsp27 Mouse mAb - Primary antibody, specific to HSPB1, Mouse IgG2b |
|---|---|
| Synonyms | Heat shock 27kDa protein antibody | 28 kDa heat shock protein antibody | CMT2F antibody | DKFZp586P1322 antibody | epididymis secretory protein Li 102 antibody | Estrogen regulated 24 kDa protein antibody | Estrogen-regulated 24 kDa protein antibody | Hea |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | HSPB1 |
| Immunogen | Recombinant Hsp27 expressed in E.coli (AA 2-205) |
| Positive Control | WB: HeLa and HepG2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Mouse anti Human Hsp27 Antibody, Monoclonal (D2), could be used for WB and so on. Application: WB: 0.01-2µg/mL Protein Function Involved in stress resistance and actin organization. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Hsp27 Mouse mAb (Ab108837) - Western Blot
All lanes: Hsp27 Mouse mAb (Ab108837) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 1.35 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201911 | Certificate of Analysis | Dec 06, 2023 | Ab108837 |
| ZJ23F1201910 | Certificate of Analysis | Dec 06, 2023 | Ab108837 |
| ZJ23F1201909 | Certificate of Analysis | Dec 06, 2023 | Ab108837 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Wang RE, Kao JL, Hilliard CA, Pandita RK, Roti Roti JL, Hunt CR, Taylor JS.. (2009) Inhibition of heat shock induction of heat shock protein 70 and enhancement of heat shock protein 27 phosphorylation by quercetin derivatives.. J Med Chem, 52 (7): (1912-1921). [PMID:19296652] [10.1021/jm801445c] |
| 3. Zhong B, Chennamaneni S, Lama R, Yi X, Geldenhuys WJ, Pink JJ, Dowlati A, Xu Y, Zhou A, Su B.. (2013) Synthesis and anticancer mechanism investigation of dual Hsp27 and tubulin inhibitors.. J Med Chem, 56 (13): (5306-5320). [PMID:23767669] [10.1021/jm4004736] |
| 4. Idippily ND, Zheng Q, Gan C, Quamine A, Ashcraft MM, Zhong B, Su B.. (2017) Copalic acid analogs down-regulate androgen receptor and inhibit small chaperone protein.. Bioorg Med Chem Lett, 27 (11): (2292-2295). [PMID:28442254] [10.1016/j.bmcl.2017.04.046] |
| 5. Crane EA, Heydenreuter W, Beck KR, Strajhar P, Vomacka J, Smiesko M, Mons E, Barth L, Neuburger M, Vedani A, Odermatt A, Sieber SA, Gademann K.. (2019) Profiling withanolide A for therapeutic targets in neurodegenerative diseases.. Bioorg Med Chem, 27 (12): (2508-2520). [PMID:30929949] [10.1016/j.bmc.2019.03.022] |
| 6. Huang MR, Hsu YL, Lin TC, Cheng TJ, Li LW, Tseng YW, Chou YS, Liu JH, Pan SH, Fang JM, Wong CH.. (2019) Structure-guided development of purine amide, hydroxamate, and amidoxime for the inhibition of non-small cell lung cancer.. Eur J Med Chem, 181 (111551-111551). [PMID:31376567] [10.1016/j.ejmech.2019.07.054] |
| 7. Stokoe, D D, Engel, K K, Campbell, D G DG, Cohen, P P and Gaestel, M M.. (1992) Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins.. FEBS letters, (30): [PMID:1332886] |
| 8. Kato, K K and 5 more authors.. (1992) Copurification of small heat shock protein with alpha B crystallin from human skeletal muscle.. The Journal of biological chemistry, (15): [PMID:1560006] |
| 9. Landry, J J and 6 more authors.. (1992) Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II.. The Journal of biological chemistry, (15): [PMID:1730670] |
| 10. Mendelsohn, M E ME, Zhu, Y Y and O'Neill, S S.. (1991) The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1763035] |
| 11. Carper, S W SW, Rocheleau, T A TA and Storm, F K FK.. (1990) cDNA sequence of a human heat shock protein HSP27.. Nucleic acids research, (11): [PMID:2243808] |
| 12. Strahler, J R JR and 9 more authors.. (1990) Identification of two related markers for common acute lymphoblastic leukemia as heat shock proteins.. The Journal of clinical investigation, [PMID:2295696] |
| 13. Fuqua, S A SA, Blum-Salingaros, M M and McGuire, W L WL.. (1989) Induction of the estrogen-regulated "24K" protein by heat shock.. Cancer research, (1): [PMID:2743305] |
| 14. Hickey, E E and 5 more authors.. (1986) Sequence and organization of genes encoding the human 27 kDa heat shock protein.. Nucleic acids research, (27): [PMID:3714473] |
| 15. Faucher, C C and 6 more authors.. (1993) The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein.. The Journal of biological chemistry, (15): [PMID:8325890] |
| 16. Clifton, A D AD, Young, P R PR and Cohen, P P.. (1996) A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress.. FEBS letters, (2): [PMID:8774846] |
| 17. Yu, W W and 9 more authors.. (1997) Large-scale concatenation cDNA sequencing.. Genome research, [PMID:9110174] |
| 18. Liu, C C, Gilmont, R R RR, Benndorf, R R and Welsh, M J MJ.. (2000) Identification and characterization of a novel protein from Sertoli cells, PASS1, that associates with mammalian small stress protein hsp27.. The Journal of biological chemistry, (23): [PMID:10751411] |
| 19. Hino, M M, Kurogi, K K, Okubo, M A MA, Murata-Hori, M M and Hosoya, H H.. (2000) Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.. Biochemical and biophysical research communications, (29): [PMID:10777697] |
| 20. Benndorf, R R and 8 more authors.. (2001) HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27).. The Journal of biological chemistry, (20): [PMID:11342557] |
| 21. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 22. Evgrafov, Oleg V OV and 25 more authors.. (2004) Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.. Nature genetics, [PMID:15122254] |
| 23. De Souza, A I AI and 5 more authors.. (2005) Heat shock protein 27 is associated with freedom from graft vasculopathy after human cardiac transplantation.. Circulation research, (22): [PMID:15976317] |
| 24. Leong, Wai Fook WF and Chow, Vincent T K VT.. (2006) Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.. Cellular microbiology, [PMID:16548883] |
| 25. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 26. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 27. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087] |
| 28. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 29. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 30. Houlden, H H and 5 more authors.. (2008) Mutations in the HSP27 (HSPB1) gene cause dominant, recessive, and sporadic distal HMN/CMT type 2.. Neurology, (18): [PMID:18832141] |
| 31. Ikeda, Yoshihisa Y and 5 more authors.. (2009) A clinical phenotype of distal hereditary motor neuronopathy type II with a novel HSPB1 mutation.. Journal of the neurological sciences, (15): [PMID:18952241] |
| 32. Kostenko, Sergiy S, Johannessen, Mona M and Moens, Ugo U.. (2009) PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5.. Cellular signalling, [PMID:19166925] |
| 33. Vos, Michel J MJ, Kanon, Bart B and Kampinga, Harm H HH.. (2009) HSPB7 is a SC35 speckle resident small heat shock protein.. Biochimica et biophysica acta, [PMID:19464326] |
| 34. Almeida-Souza, Leonardo L and 11 more authors.. (2010) Increased monomerization of mutant HSPB1 leads to protein hyperactivity in Charcot-Marie-Tooth neuropathy.. The Journal of biological chemistry, (23): [PMID:20178975] |
| 35. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 36. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287] |
| 37. Capponi, Simona S and 8 more authors.. (2011) HSPB1 and HSPB8 in inherited neuropathies: study of an Italian cohort of dHMN and CMT2 patients.. Journal of the peripheral nervous system : JPNS, [PMID:22176143] |
| 38. Lin, Kon-Ping KP and 8 more authors.. (2011) The mutational spectrum in a cohort of Charcot-Marie-Tooth disease type 2 among the Han Chinese in Taiwan.. PloS one, [PMID:22206013] |
| 39. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 40. Nefedova, Victoria V VV, Datskevich, Petr N PN, Sudnitsyna, Maria V MV, Strelkov, Sergei V SV and Gusev, Nikolai B NB.. (2013) Physico-chemical properties of R140G and K141Q mutants of human small heat shock protein HspB1 associated with hereditary peripheral neuropathies.. Biochimie, [PMID:23643870] |
| 41. Holmgren, Anne A and 6 more authors.. (2013) Charcot-Marie-Tooth causing HSPB1 mutations increase Cdk5-mediated phosphorylation of neurofilaments.. Acta neuropathologica, [PMID:23728742] |
| 42. Nefedova, Victoria V VV, Sudnitsyna, Maria V MV, Strelkov, Sergei V SV and Gusev, Nikolai B NB.. (2013) Structure and properties of G84R and L99M mutants of human small heat shock protein HspB1 correlating with motor neuropathy.. Archives of biochemistry and biophysics, (1): [PMID:23948568] |
| 43. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 44. Muranova, Lydia K LK, Weeks, Stephen D SD, Strelkov, Sergei V SV and Gusev, Nikolai B NB.. (2015) Characterization of Mutants of Human Small Heat Shock Protein HspB1 Carrying Replacements in the N-Terminal Domain and Associated with Hereditary Motor Neuron Diseases.. PloS one, [PMID:25965061] |
| 45. Echaniz-Laguna, Andoni A and 20 more authors.. (2017) Axonal Neuropathies due to Mutations in Small Heat Shock Proteins: Clinical, Genetic, and Functional Insights into Novel Mutations.. Human mutation, [PMID:28144995] |
| 46. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
