IDH1 Mouse mAb - Primary antibody, specific to IDH1, Mouse IgG1

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human,Mouse
  • Isotype: Mouse IgG2a
  • Clone Number: C12
  • Conjugation: Unconjugated
Item Number
Ab109324
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab109324-10μl
10μl
In stock
$69.90
Ab109324-50μl
50μl
In stock
$209.90
Ab109324-100μl
100μl
In stock
$299.90
Ab109324-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

mAb (C12); Mouse anti Human IDH1 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameIDH1 Mouse mAb - Primary antibody, specific to IDH1, Mouse IgG1
SynonymsCytosolic NADP isocitrate dehydrogenase antibody | Cytosolic NADP-isocitrate dehydrogenase antibody | Epididymis luminal protein 216 antibody | Epididymis secretory protein Li 26 antibody | HEL-216 antibody | HEL-S-26 antibody | ICDH antibody | IDCD antib
Specifications & PurityCarrier Free, ExactAb™, Validated, High Performance, See COA
Host speciesMouse
SpecificityIDH1
ImmunogenRecombinant IDH1 expressed in E.coli (AA 74-333).
Positive ControlWB: Hep G2 and NIH/3T3 cell lysates. IHC: Human colorectal cancer, stomach, kidney, prostate, glioma tissues.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human IDH1 Antibody, Monoclonal antibody (C12), could be used for WB,IHC and so on.
Application:
WB: 0.01-2µg/mL
IHC:5-30µg/mL
Protein Function

Product Properties

IsotypeMouse IgG2a
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A + Protein G purified
FormLiquid
ConcentrationSee COA
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

IDH1 Mouse mAb (Ab109324) - Western Blot
All lanes: IDH1 Mouse mAb (Ab109324) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 47 kDa
Observed band size: 44 kDa
Exposure time: 25.4 s

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Glioma Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Associated Targets(Human)

IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (40980 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IHC

5-30 µg/mL

WB

0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303945Certificate of AnalysisMar 31, 2024 Ab109324
ZJ24F0303944Certificate of AnalysisMar 31, 2024 Ab109324
ZJ24F0303943Certificate of AnalysisMar 31, 2024 Ab109324

Related Documents

References

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