mAb (D15); Mouse anti Human IGFBP3 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109567-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab109567-20μl | 20μl | In stock | $99.90 | |
| Ab109567-50μl | 50μl | In stock | $189.90 | |
| Ab109567-100μl | 100μl | In stock | $299.90 | |
| Ab109567-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
mAb (D15); Mouse anti Human IGFBP3 Antibody; WB; Unconjugated
| Product Name | IGFBP3 Mouse mAb - Primary antibody, specific to IGFBP3, Mouse IgG1 |
|---|---|
| Synonyms | Acid stable subunit of the 140 K IGF complex antibody; Binding protein 29 antibody; Binding protein 53 antibody; BP 53 antibody; BP53 antibody; Growth hormone dependent binding proteinantibody; IBP 3 antibody; IBP3 antibody; IGF binding protein 3 antibody |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | IGFBP3 |
| Immunogen | Recombinant IGFBP3 expressed in E.coli (AA 135-277) |
| Positive Control | WB: A431 and Hep G2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human IGFBP3 Antibody, Monoclonal (D15), could be used for WB and so on. Application: WB: 0.5-2µg/mL Protein Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
IGFBP3 Mouse mAb (Ab109567) - Western Blot
All lanes: IGFBP3 Mouse mAb (Ab109567) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 37 kDa
Exposure time: 9.1 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 0.5-2µg/mL |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201972 | Certificate of Analysis | Dec 13, 2023 | Ab109567 |
| ZJ23F1201973 | Certificate of Analysis | Dec 13, 2023 | Ab109567 |
| ZJ23F1201974 | Certificate of Analysis | Dec 13, 2023 | Ab109567 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] |
| 2. Evert Njomen,Hedeel G Evans,Samanthi H Gedara,Deborah L Heyl. (2015-07-29) Humanin Peptide Binds to Insulin-Like Growth Factor-Binding Protein 3 (IGFBP3) and Regulates Its Interaction with Importin-β.. Protein and peptide letters, 22 ((10)): (869-876). [PMID:26216267] |
| 3. Cubbage, M L ML, Suwanichkul, A A and Powell, D R DR.. (1990) Insulin-like growth factor binding protein-3. Organization of the human chromosomal gene and demonstration of promoter activity.. The Journal of biological chemistry, (25): [PMID:1695633] |
| 4. Zapf, J J and 7 more authors.. (1990) Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia.. The Journal of biological chemistry, (5): [PMID:1697583] |
| 5. Roghani, M M, Segovia, B B, Whitechurch, O O and Binoux, M M.. (1991) Purification from human cerebrospinal fluid of insulin-like growth factor binding proteins (IGFBPs). Isolation of IGFBP-2, an altered form of IGFBP-3 and a new IGFBP species.. Growth regulation, [PMID:1726837] |
| 6. Wood, W I WI and 7 more authors.. (1988) Cloning and expression of the growth hormone-dependent insulin-like growth factor-binding protein.. Molecular endocrinology (Baltimore, Md.), [PMID:2464130] |
| 7. Thweatt, R R, Fleischmann, R R and Goldstein, S S.. (1993) Analysis of the primary structure of insulin-like growth factor binding protein-3 cDNA from Werner syndrome fibroblasts.. DNA sequence : the journal of DNA sequencing and mapping, [PMID:7508771] |
| 8. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 9. Ikonen, Maaria M and 7 more authors.. (2003) Interaction between the Alzheimer's survival peptide humanin and insulin-like growth factor-binding protein 3 regulates cell survival and apoptosis.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:14561895] |
| 10. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718] |
| 11. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 12. . (2005) Mecasermin rinfabate: insulin-like growth factor-I/insulin-like growth factor binding protein-3, mecaserimin rinfibate, rhIGF-I/rhIGFBP-3.. Drugs in R&D, [PMID:15777106] |
| 13. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 14. Chang, Yoon Soo YS and 7 more authors.. (2006) 9-cis retinoic acid induces insulin-like growth factor binding protein-3 through DR-8 retinoic acid responsive elements.. Cancer biology & therapy, [PMID:16760641] |
| 15. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 16. Williams, Rachel M RM, McDonald, Anna A, O'Savage, Martin M and Dunger, David B DB.. (2008) Mecasermin rinfabate: rhIGF-I/rhIGFBP-3 complex: iPLEX.. Expert opinion on drug metabolism & toxicology, [PMID:18363546] |
| 17. Muzumdar, Radhika H RH and 12 more authors.. (2009) Humanin: a novel central regulator of peripheral insulin action.. PloS one, (22): [PMID:19623253] |
| 18. and Kemp, Stephen F SF.. (2009) Insulin-like growth factor-I deficiency in children with growth hormone insensitivity: current and future treatment options.. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, [PMID:19627167] |
| 19. Lue, YanHe Y and 10 more authors.. (2010) Opposing roles of insulin-like growth factor binding protein 3 and humanin in the regulation of testicular germ cell apoptosis.. Endocrinology, [PMID:19952275] |
| 20. Ingermann, Angela R AR and 11 more authors.. (2010) Identification of a novel cell death receptor mediating IGFBP-3-induced anti-tumor effects in breast and prostate cancer.. The Journal of biological chemistry, (24): [PMID:20353938] |
| 21. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039] |
