| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109766-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab109766-20μl | 20μl | In stock | $99.90 | |
| Ab109766-50μl | 50μl | In stock | $189.90 | |
| Ab109766-100μl | 100μl | In stock | $299.90 | |
| Ab109766-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (3#); Mouse anti Human IL-1 alpha Antibody; WB; Unconjugated
| Product Name | IL-1 alpha Mouse mAb - Primary antibody, specific to IL1A, Mouse IgG2b |
|---|---|
| Synonyms | BAF antibody | FAF antibody | Hematopoietin 1 antibody | Hematopoietin-1 antibody | IL 1 alpha antibody | IL 1A antibody | IL-1 alpha antibody | Il-1a antibody | IL1 ALPHA antibody | IL1 antibody | IL1A antibody | IL1A_HUMAN antibody | IL1F1 antibody | In |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | IL1A |
| Immunogen | Recombinant IL1a expressed in E.coli (AA 113-271). |
| Positive Control | WB: RAW 264.7 and CTLL-2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human IL-1 alpha Antibody, Monoclonal (3#), could be used for WB and so on. Application WB: 0.5-2µg/mL Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
IL-1 alpha Mouse mAb (Ab109766) - Western Blot
All lanes: IL-1 alpha Mouse mAb (Ab109766) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 32 kDa
Exposure time: 100.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 0.5-2µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201902 | Certificate of Analysis | Dec 06, 2023 | Ab109766 |
| ZJ23F1201901 | Certificate of Analysis | Dec 06, 2023 | Ab109766 |
| ZJ23F1201900 | Certificate of Analysis | Dec 06, 2023 | Ab109766 |
| 1. Graves, B J BJ and 5 more authors.. (1990) Structure of interleukin 1 alpha at 2.7-A resolution.. Biochemistry, (20): [PMID:2346741] |
| 2. March, C J CJ and 9 more authors.. (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.. Nature, [PMID:2989698] |
| 3. Furutani, Y Y and 9 more authors.. (1985) Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.. Nucleic acids research, (26): [PMID:2994016] |
| 4. Zsebo, K M KM and 6 more authors.. (1988) Effects of hematopoietin-1 and interleukin 1 activities on early hematopoietic cells of the bone marrow.. Blood, [PMID:3281727] |
| 5. Gubler, U U and 9 more authors.. (1986) Recombinant human interleukin 1 alpha: purification and biological characterization.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:3485152] |
| 6. Furutani, Y Y and 6 more authors.. (1986) Complete nucleotide sequence of the gene for human interleukin 1 alpha.. Nucleic acids research, (25): [PMID:3486405] |
| 7. Nishida, T T and 7 more authors.. (1987) cDNA cloning of IL-1 alpha and IL-1 beta from mRNA of U937 cell line.. Biochemical and biophysical research communications, (27): [PMID:3493774] |
| 8. Stevenson, F T FT, Bursten, S L SL, Fanton, C C, Locksley, R M RM and Lovett, D H DH.. (1993) The 31-kDa precursor of interleukin 1 alpha is myristoylated on specific lysines within the 16-kDa N-terminal propiece.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8346241] |
| 9. Nicklin, Martin J H MJ and 5 more authors.. (2002) A sequence-based map of the nine genes of the human interleukin-1 cluster.. Genomics, [PMID:11991722] |
| 10. Spiekstra, S W SW and 10 more authors.. (2005) Induction of cytokine (interleukin-1alpha and tumor necrosis factor-alpha) and chemokine (CCL20, CCL27, and CXCL8) alarm signals after allergen and irritant exposure.. Experimental dermatology, [PMID:15679580] |
| 11. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 12. Brikos, Constantinos C, Wait, Robin R, Begum, Shajna S, O'Neill, Luke A J LA and Saklatvala, Jeremy J.. (2007) Mass spectrometric analysis of the endogenous type I interleukin-1 (IL-1) receptor signaling complex formed after IL-1 binding identifies IL-1RAcP, MyD88, and IRAK-4 as the stable components.. Molecular & cellular proteomics : MCP, [PMID:17507369] |
| 13. Mohan, Sepuru K SK and Yu, Chin C.. (2011) The IL1alpha-S100A13 heterotetrameric complex structure: a component in the non-classical pathway for interleukin 1alpha secretion.. The Journal of biological chemistry, (22): [PMID:21270123] |
| 14. Cronstein, Bruce N BN and Sunkureddi, Prashanth P.. (2013) Mechanistic aspects of inflammation and clinical management of inflammation in acute gouty arthritis.. Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, [PMID:23319019] |
| 15. Tran, Tran H TH, Pham, Jacqueline T JT, Shafeeq, Hira H, Manigault, Kendra R KR and Arya, Vibhuti V.. (2013) Role of interleukin-1 inhibitors in the management of gout.. Pharmacotherapy, [PMID:23553601] |
| 16. Cohen, Idan I and 24 more authors.. (2015) IL-1α is a DNA damage sensor linking genotoxic stress signaling to sterile inflammation and innate immunity.. Scientific reports, (6): [PMID:26439902] |
| 17. Kotenko, S V SV, Bulenkov, M T MT, Veĭko, V P VP, Epishin, S M SM and Lomakin, I B IB.. (1989) [Cloning of the cDNA coding for human prointerleukin-1 alpha and prointerleukin-1 beta].. Doklady Akademii nauk SSSR, [PMID:2635664] |
