mAb(1H3-15); Mouse anti Human IL-10 Antibody; Detection Antibody, ELISA, CLIA, LF, GICA, FIA, FACS; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109817-10μg | 10μg | In stock | $66.90 | |
| Ab109817-50μg | 50μg | In stock | $199.90 | |
| Ab109817-100μg | 100μg | In stock | $299.90 | |
| Ab109817-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,599.90 |
mAb(1H3-15); Mouse anti Human IL-10 Antibody; Detection Antibody, ELISA, CLIA, LF, GICA, FIA, FACS; Unconjugated
| Product Name | IL-10 Mouse mAb - Primary antibody, specific to IL10, Mouse IgG2b |
|---|---|
| Synonyms | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF; CSIF antibody; Cytokine synthesis inhibitory factor antibody; GVHDS antibody; IL 10 antibody; IL-10 antibody; IL10 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | IL10 |
| Immunogen | Recombinant Human IL-10 Protein |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human IL-10 Antibody, Monoclonal (1H3-15), is a Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA, FACS. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 25.9 kDa (Light Chain) & 53.1 kDa (Heavy Chain), under reducing conditions; 183.4 kDa, under non-reducing conditions. |
| Purification Method | Affinity chromatography |
| Matched Pairs | [Ab109816] and [Ab109811] IL-10 Mouse mAb as Capture antibody in ELISA, CLIA, LF, GICA, FIA, FACS |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 37°C short term（7 days）Store at 4°C short term（30 days）. Store at -20±5°C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Sandwich ELISA - IL-10 protein standard curve
Human IL-10 protein was serially diluted 2-fold and captured by IL-10 Mouse mAb (Ab109816) coated on a Clear Polystyrene Microplate (W291037). IL-10 Mouse mAb (Ab109817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab109816 & Ab109817) in CLIA
·Capture antibody: IL-10 Mouse mAb (Ab109816)
·Detection antibody: IL-10 Mouse mAb (Ab109817)
Sandwich ELISA - IL-10 protein standard curve
Human IL-10 protein was serially diluted 2-fold and captured by IL-10 Mouse mAb (Ab109811) coated on a Clear Polystyrene Microplate (W291037). IL-10 Mouse mAb (Ab109817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab109811 & Ab109817) in CLIA
·Capture antibody: IL-10 Mouse mAb (Ab109811)
·Detection antibody: IL-10 Mouse mAb (Ab109817)
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| CLIA | 100μg/4μg AE |
| FLFIA | 1.0 mg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404435 | Certificate of Analysis | Apr 11, 2024 | Ab109817 |
| ZJ24F0404434 | Certificate of Analysis | Apr 11, 2024 | Ab109817 |
| ZJ24F0404433 | Certificate of Analysis | Apr 11, 2024 | Ab109817 |
| 1. Vieira, P P and 9 more authors.. (1991) Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1847510] |
| 2. de Waal Malefyt, R R, Abrams, J J, Bennett, B B, Figdor, C G CG and de Vries, J E JE.. (1991) Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.. The Journal of experimental medicine, (1): [PMID:1940799] |
| 3. Walter, M R MR and Nagabhushan, T L TL.. (1995) Crystal structure of interleukin 10 reveals an interferon gamma-like fold.. Biochemistry, (26): [PMID:7547951] |
| 4. Zdanov, A A and 5 more authors.. (1995) Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma.. Structure (London, England : 1993), (15): [PMID:8590020] |
| 5. Gesser, B B and 8 more authors.. (1997) Identification of functional domains on human interleukin 10.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:9405662] |
| 6. Josephson, K K, Logsdon, N J NJ and Walter, M R MR.. (2001) Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site.. Immunity, [PMID:11485736] |
| 7. van der Linde, K K and 6 more authors.. (2003) A Gly15Arg mutation in the interleukin-10 gene reduces secretion of interleukin-10 in Crohn disease.. Scandinavian journal of gastroenterology, [PMID:12825869] |
| 8. Lin, Ming-Tseh MT and 6 more authors.. (2003) Relation of an interleukin-10 promoter polymorphism to graft-versus-host disease and survival after hematopoietic-cell transplantation.. The New England journal of medicine, (4): [PMID:14657427] |
| 9. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 10. Yoon, Sung Il SI, Jones, Brandi C BC, Logsdon, Naomi J NJ and Walter, Mark R MR.. (2005) Same structure, different function crystal structure of the Epstein-Barr virus IL-10 bound to the soluble IL-10R1 chain.. Structure (London, England : 1993), [PMID:15837194] |
| 11. Yoon, Sung Il SI, Logsdon, Naomi J NJ, Sheikh, Faruk F, Donnelly, Raymond P RP and Walter, Mark R MR.. (2006) Conformational changes mediate interleukin-10 receptor 2 (IL-10R2) binding to IL-10 and assembly of the signaling complex.. The Journal of biological chemistry, (17): [PMID:16982608] |
| 12. El Kasmi, Karim C KC and 9 more authors.. (2007) Cutting edge: A transcriptional repressor and corepressor induced by the STAT3-regulated anti-inflammatory signaling pathway.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:18025162] |
| 13. Windsor, W T WT and 9 more authors.. (1993) Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10.. Biochemistry, (31): [PMID:8364028] |
| 14. Zdanov, A A, Schalk-Hihi, C C and Wlodawer, A A.. (1996) Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor.. Protein science : a publication of the Protein Society, [PMID:8897595] |
