| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab006917-10μl | 10μl | In stock | $66.90 |
mAb (C11); Mouse anti Human IL-33 Antibody; WB, IHC; Unconjugated
| Product Name | IL-33 Mouse mAb - Primary antibody, specific to IL33, Mouse IgG2a |
|---|---|
| Synonyms | IL-1F11 | IL-33 | Interleukin-1 family member 11 | NF-HEV | Nuclear factor from high endothelial venules |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | IL33 |
| Immunogen | Recombinant IL33 expressed in E.coli (AA 112-270). |
| Positive Control | WB: Jurkat, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human Stomach cancer Tissue, Human Prostate cancer Tissue, Human Colorectal cancer Tissue, Human Breast cancer Tissue, Human Liver Tissue. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Mouse anti Human IL-33 Antibody, Monoclonal antibody (C11), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G affinity chromatography |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
IL-33 Mouse mAb (Ab006917) - Western Blot
All lanes: IL-33 Mouse mAb (Ab006917) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 150.0 s
IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Stomach cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-33 Mouse mAb (Ab006917) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 30 µg/mL IL-33 Mouse mAb (Ab006917)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.01-3 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102402 | Certificate of Analysis | Jan 10, 2024 | Ab006917 |
| ZJ24F0102401 | Certificate of Analysis | Jan 10, 2024 | Ab006917 |
| ZJ24F0102400 | Certificate of Analysis | Jan 10, 2024 | Ab006917 |
| 1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e] |
| 2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039] [10.1021/op500134e] |
| 3. Osbourn M, Soares DC, Vacca F, Cohen ES, Scott IC, Gregory WF, Smyth DJ, Toivakka M, Kemter AM, le Bihan T et al.. (2017) HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.. Immunity, 47 (4): (739-751.e5). [PMID:29045903] [10.1021/op500134e] |
| 4. Onda, H H and 7 more authors.. (1999) Identification of genes differentially expressed in canine vasospastic cerebral arteries after subarachnoid hemorrhage.. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, [PMID:10566975] |
| 5. Baekkevold, Espen S ES and 9 more authors.. (2003) Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules.. The American journal of pathology, [PMID:12819012] |
| 6. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053] |
| 7. Schmitz, Jochen J and 12 more authors.. (2005) IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines.. Immunity, [PMID:16286016] |
| 8. Carriere, Virginie V and 7 more authors.. (2007) IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:17185418] |
| 9. Komai-Koma, Mousa M and 5 more authors.. (2007) IL-33 is a chemoattractant for human Th2 cells.. European journal of immunology, [PMID:17853410] |
| 10. Küchler, Axel M AM and 8 more authors.. (2008) Nuclear interleukin-33 is generally expressed in resting endothelium but rapidly lost upon angiogenic or proinflammatory activation.. The American journal of pathology, [PMID:18787100] |
| 11. Moussion, Christine C, Ortega, Nathalie N and Girard, Jean-Philippe JP.. (2008) The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel 'alarmin'?. PloS one, (6): [PMID:18836528] |
| 12. Cayrol, Corinne C and Girard, Jean-Philippe JP.. (2009) The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:19439663] |
| 13. Talabot-Ayer, Dominique D, Lamacchia, Céline C, Gabay, Cem C and Palmer, Gaby G.. (2009) Interleukin-33 is biologically active independently of caspase-1 cleavage.. The Journal of biological chemistry, (17): [PMID:19465481] |
| 14. Lüthi, Alexander U AU and 11 more authors.. (2009) Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases.. Immunity, (17): [PMID:19559631] |
| 15. Hayakawa, Morisada M and 5 more authors.. (2009) Mature interleukin-33 is produced by calpain-mediated cleavage in vivo.. Biochemical and biophysical research communications, (11): [PMID:19596270] |
| 16. Lingel, Andreas and 7 more authors.. (2009) Structure of IL-33 and its interaction with the ST2 and IL-1RAcP receptors--insight into heterotrimeric IL-1 signaling complexes.. Structure (London, England : 1993), (14): [PMID:19836339] |
| 17. Hong, Jaewoo J and 10 more authors.. (2011) Identification of constitutively active interleukin 33 (IL-33) splice variant.. The Journal of biological chemistry, (3): [PMID:21454686] |
| 18. Ali, Shafaqat S and 6 more authors.. (2011) The dual function cytokine IL-33 interacts with the transcription factor NF-κB to dampen NF-κB-stimulated gene transcription.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:21734074] |
| 19. Kakkar, Rahul R, Hei, Hillary H, Dobner, Stephan S and Lee, Richard T RT.. (2012) Interleukin 33 as a mechanically responsive cytokine secreted by living cells.. The Journal of biological chemistry, (24): [PMID:22215666] |
| 20. Lefrançais, Emma E and 6 more authors.. (2012) IL-33 is processed into mature bioactive forms by neutrophil elastase and cathepsin G.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22307629] |
| 21. Liu, Xi X and 7 more authors.. (2013) Structural insights into the interaction of IL-33 with its receptors.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:23980170] |
