| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab110326-10μl | 10μl | In stock | $66.90 | |
| Ab110326-50μl | 50μl | In stock | $189.90 | |
| Ab110326-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab110326-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
pAb; Rabbit anti Human IL-4 Antibody; WB; Unconjugated
| Product Name | IL-4 Antibody - Primary antibody, specific to IL-4, Rabbit IgG |
|---|---|
| Synonyms | B cell growth factor 1 antibody | B cell IgG differentiation factor antibody | B Cell Stimulatory Factor 1 antibody | B-cell stimulatory factor 1 antibody | BCGF 1 antibody | BCGF1 antibody | Binetrakin antibody | BSF-1 antibody | BSF1antibody | IGG1 indu |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | IL-4 |
| Immunogen | A synthetic peptide derived from human IL4 (AA 91-104) |
| Positive Control | WB: Recombinant Human IL-4 protein, Human tonsil lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human IL-4 Antibody, Polyclonal, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
IL-4 Antibody (Ab110326) - Western Blot
All lanes: IL-4 Antibody (Ab110326) at 1/1000 dilution
Samples: Recombinant Human IL-4 protein (rp156008) at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 1.0 s
IL-4 Antibody (Ab110326) - Western Blot
Western blot analysis of IL-4 in Human tonsil lysates using IL-4 Antibody (Ab110326).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404336 | Certificate of Analysis | Apr 10, 2024 | Ab110326 |
| ZJ24F0404337 | Certificate of Analysis | Apr 10, 2024 | Ab110326 |
| 1. Hart TK, Blackburn MN, Brigham-Burke M, Dede K, Al-Mahdi N, Zia-Amirhosseini P, Cook RM. (2002) Preclinical efficacy and safety of pascolizumab (SB 240683): a humanized anti-interleukin-4 antibody with therapeutic potential in asthma.. Clin Exp Immunol, 130 (1): (93-100). [PMID:12296858] [10.1021/op500134e] |
| 2. Walter, M R MR and 9 more authors.. (1992) Crystal structure of recombinant human interleukin-4.. The Journal of biological chemistry, (5): [PMID:1400355] |
| 3. Wlodawer, A A, Wlodaver, A A, Pavlovsky, A A and Gustchina, A A.. (1992) Crystal structure of human recombinant interleukin-4 at 2.25 A resolution.. FEBS letters, (31): [PMID:1511746] |
| 4. Powers, R R and 5 more authors.. (1992) 1H, 15N, 13C, and 13CO assignments of human interleukin-4 using three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy.. Biochemistry, (5): [PMID:1567880] |
| 5. Garrett, D S DS and 5 more authors.. (1992) Determination of the secondary structure and folding topology of human interleukin-4 using three-dimensional heteronuclear magnetic resonance spectroscopy.. Biochemistry, (5): [PMID:1567881] |
| 6. Smith, L J LJ and 6 more authors.. (1992) Human interleukin 4. The solution structure of a four-helix bundle protein.. Journal of molecular biology, (20): [PMID:1569578] |
| 7. Redfield, C C and 6 more authors.. (1991) Secondary structure and topology of human interleukin 4 in solution.. Biochemistry, (19): [PMID:1932028] |
| 8. Curtis, B M BM and 8 more authors.. (1991) Experimental and theoretical studies of the three-dimensional structure of human interleukin-4.. Proteins, [PMID:1946344] |
| 9. Carr, C C, Aykent, S S, Kimack, N M NM and Levine, A D AD.. (1991) Disulfide assignments in recombinant mouse and human interleukin 4.. Biochemistry, (12): [PMID:1993171] |
| 10. Arai, N N and 9 more authors.. (1989) Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2535858] |
| 11. Yokota, T T and 8 more authors.. (1986) Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3016727] |
| 12. Eder, A A, Krafft-Czepa, H H and Krammer, P H PH.. (1988) The 5' region of the human interleukin 4 gene: structure and potential regulatory elements.. Nucleic acids research, (25): [PMID:3257560] |
| 13. Smith, L J LJ and 8 more authors.. (1994) Comparison of four independently determined structures of human recombinant interleukin-4.. Nature structural biology, [PMID:7664036] |
| 14. Klein, S C SC, Golverdingen, J G JG, Bouwens, A G AG, Tilanus, M G MG and de Weger, R A RA.. (1995) An alternatively spliced interleukin 4 form in lymphoid cells.. Immunogenetics, [PMID:7806280] |
| 15. Müller, T T, Dieckmann, T T, Sebald, W W and Oschkinat, H H.. (1994) Aspects of receptor binding and signalling of interleukin-4 investigated by site-directed mutagenesis and NMR spectroscopy.. Journal of molecular biology, (8): [PMID:8151703] |
| 16. Hage, T T, Sebald, W W and Reinemer, P P.. (1999) Crystal structure of the interleukin-4/receptor alpha chain complex reveals a mosaic binding interface.. Cell, (16): [PMID:10219247] |
| 17. Hülsmeyer, M M, Scheufler, C C and Dreyer, M K MK.. (2001) Structure of interleukin 4 mutant E9A suggests polar steering in receptor-complex formation.. Acta crystallographica. Section D, Biological crystallography, [PMID:11526337] |
| 18. Zee, Robert Y L RY and 6 more authors.. (2004) Polymorphism in the P-selectin and interleukin-4 genes as determinants of stroke: a population-based, prospective genetic analysis.. Human molecular genetics, (15): [PMID:14681304] |
| 19. LaPorte, Sherry L SL and 7 more authors.. (2008) Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system.. Cell, (25): [PMID:18243101] |
| 20. Powers, R R and 5 more authors.. (1992) Three-dimensional solution structure of human interleukin-4 by multidimensional heteronuclear magnetic resonance spectroscopy.. Science (New York, N.Y.), (19): [PMID:1609277] |
| 21. Malabarba, M G MG and 7 more authors.. (1995) Activation of JAK3, but not JAK1, is critical to interleukin-4 (IL4) stimulated proliferation and requires a membrane-proximal region of IL4 receptor alpha.. The Journal of biological chemistry, (21): [PMID:7721895] |
