IL-6 Mouse mAb - Primary antibody, for CLIA,LF,GICA,Capture antibody various applications, from Human, generated in Mouse, specific to IL-6, IgG

    Application:
  • Capture Antibody
  • CLIA
  • ELISA
  • GICA
  • LF
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Clone Number: 5A10
  • Conjugation: Unconjugated
Item Number
Ab110369
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab110369-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab110369-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$117.90
Ab110369-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$177.90
Ab110369-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$387.90

mAb (5A10); Mouse anti Human IL-6 Antibody; Capture Antibody, CLIA, GICA, LF; Unconjugated

Basic Description

Product NameIL-6 Mouse mAb - Primary antibody, for CLIA,LF,GICA,Capture antibody various applications, from Human, generated in Mouse, specific to IL-6, IgG
SynonymsB-cell differentiation factor | B-cell stimulatory factor 2 | BSF2 | BSF-2 | CDF | CTL differentiation factor | HSF | hybridoma growth factor | IFNB2 | IFN-beta-2 | IL6 | IL-6 | Interferon beta-2 | interleukin 6 (interferon, beta 2) | interleukin BSF-2 |
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host SpeciesMouse
Host speciesMouse
SpecificityIL-6
ApplicationCLIA,LF,GICA,Capture antibody
ImmunogenE. coli-derived recombinant human IL-6.
Positive ControlWB: Recombinant mature mouse IL6 protein. IHC-P: Mouse pancreas.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Validated
Product Description

Monoclonal IL-6 Antibody, 5A10, is a Capture antibody could be used for CLIA at 2.0 mg/mL.

Product Properties

Antibody TypePrimary antibody
ClonalityMonoclonal antibodies
SDS-PAGE150 kDa
Purification MethodProtein A purified
Matched Pairs[Ab110379] Monoclonal IL-6 Antibody as Detection antibody in CLIA, GICA, LF
SourceHybridoma supernatant
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20±5℃ for three years. Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

IL-6 Mouse mAb (Ab110369) - Binding ELISA
The binding activity of IL-6 Mouse mAb with IL-6 antigen. 

Associated Targets(Human)

IL6 Tclin Interleukin-6 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700659Certificate of AnalysisJul 21, 2023 Ab110369
ZJ23F0700660Certificate of AnalysisJul 21, 2023 Ab110369

Related Documents

References

1. Boulanger MJ, Chow DC, Brevnova EE, Garcia KC.  (2003)  Hexameric structure and assembly of the interleukin-6/IL-6 alpha-receptor/gp130 complex..  Science,  300  (5628): (2101-4).  [PMID:12829785] [10.1021/op500134e]
2. Shaw S, Bourne T, Meier C, Carrington B, Gelinas R, Henry A, Popplewell A, Adams R, Baker T, Rapecki S et al..  (2014)  Discovery and characterization of olokizumab: a humanized antibody targeting interleukin-6 and neutralizing gp130-signaling..  MAbs,  (3): (774-82).  [PMID:24670876] [10.1021/op500134e]
3. Kang S, Tanaka T, Narazaki M, Kishimoto T.  (2019)  Targeting Interleukin-6 Signaling in Clinic..  Immunity,  50  (4): (1007-1023).  [PMID:30995492] [10.1016/j.immuni.2019.03.026]
4. Q Y Chen.  (1992-09-01)  [Stable and efficient expression of human interleukin-6 cDNA in mammalian cells after gene transfer]..  Zhonghua zhong liu za zhi [Chinese journal of oncology],  14  ((5)): (340-344).  [PMID:1291290]
5. H C van Zaanen,R P Koopmans,L A Aarden,H J Rensink,J M Stouthard,S O Warnaar,H M Lokhorst,M H van Oers.  (1996-09-15)  Endogenous interleukin 6 production in multiple myeloma patients treated with chimeric monoclonal anti-IL6 antibodies indicates the existence of a positive feed-back loop..  The Journal of clinical investigation,  98  ((6)): (1441-1448).  [PMID:8823310]
6. Kim D, Won HY, Hwang ES, Kim YK, Choo HP..  (2017)  Synthesis of benzoxazole derivatives as interleukin-6 antagonists..  Bioorg Med Chem,  25  (12): (3127-3134).  [PMID:28442260] [10.1016/j.bmc.2017.03.072]
7. May, L T LT, Shaw, J E JE, Khanna, A K AK, Zabriskie, J B JB and Sehgal, P B PB..  (1991)  Marked cell-type-specific differences in glycosylation of human interleukin-6..  Cytokine,      [PMID:1883960]
8. Lütticken, C C, Krüttgen, A A, Möller, C C, Heinrich, P C PC and Rose-John, S S..  (1991)  Evidence for the importance of a positive charge and an alpha-helical structure of the C-terminus for biological activity of human IL-6..  FEBS letters,    (6):   [PMID:2037043]
9. Clogston, C L CL, Boone, T C TC, Crandall, B C BC, Mendiaz, E A EA and Lu, H S HS..  (1989)  Disulfide structures of human interleukin-6 are similar to those of human granulocyte colony stimulating factor..  Archives of biochemistry and biophysics,      [PMID:2472117]
10. Ming, J E JE, Cernetti, C C, Steinman, R M RM and Granelli-Piperno, A A..  (1989)  Interleukin 6 is the principal cytolytic T lymphocyte differentiation factor for thymocytes in human leukocyte conditioned medium..  The Journal of molecular and cellular immunology : JMCI,      [PMID:2610854]
11. Tonouchi, N N, Miwa, K K, Karasuyama, H H and Matsui, H H..  (1989)  Deletion of 3' untranslated region of human BSF-2 mRNA causes stabilization of the mRNA and high-level expression in mouse NIH3T3 cells..  Biochemical and biophysical research communications,    (15):   [PMID:2789513]
12. Zilberstein, A A, Ruggieri, R R, Korn, J H JH and Revel, M M..  (1986)  Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species inducible by growth-stimulatory cytokines..  The EMBO journal,      [PMID:3023045]
13. Wong, G G GG, Witek-Giannotti, J J, Hewick, R M RM, Clark, S C SC and Ogawa, M M..  (1988)  Interleukin 6: identification as a hematopoietic colony-stimulating factor..  Behring Institute Mitteilungen,      [PMID:3266463]
14. Van Damme, J J and 5 more authors..  (1988)  Separation and comparison of two monokines with lymphocyte-activating factor activity: IL-1 beta and hybridoma growth factor (HGF). Identification of leukocyte-derived HGF as IL-6..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:3279116]
15. Brakenhoff, J P JP, de Groot, E R ER, Evers, R F RF, Pannekoek, H H and Aarden, L A LA..  (1987)  Molecular cloning and expression of hybridoma growth factor in Escherichia coli..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:3320204]
16. Hirano, T T and 9 more authors..  (1986)  Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin..  Nature,      [PMID:3491322]
17. Yasukawa, K K and 6 more authors..  (1987)  Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene..  The EMBO journal,      [PMID:3500852]
18. May, L T LT, Helfgott, D C DC and Sehgal, P B PB..  (1986)  Anti-beta-interferon antibodies inhibit the increased expression of HLA-B7 mRNA in tumor necrosis factor-treated human fibroblasts: structural studies of the beta 2 interferon involved..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3538015]
19. Haegeman, G G and 5 more authors..  (1986)  Structural analysis of the sequence coding for an inducible 26-kDa protein in human fibroblasts..  European journal of biochemistry,    (15):   [PMID:3758081]
20. Breton, J J and 8 more authors..  (1995)  Structure, stability and biological properties of a N-terminally truncated form of recombinant human interleukin-6 containing a single disulfide bond..  European journal of biochemistry,    (15):   [PMID:7851440]
21. Nishimura, C C, Watanabe, A A, Gouda, H H, Shimada, I I and Arata, Y Y..  (1996)  Folding topologies of human interleukin-6 and its mutants as studied by NMR spectroscopy..  Biochemistry,    (9):   [PMID:8555185]
22. Somers, W W, Stahl, M M and Seehra, J S JS..  (1997)  1.9 A crystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling..  The EMBO journal,    (3):   [PMID:9118960]
23. Xu, G Y GY and 6 more authors..  (1997)  Solution structure of recombinant human interleukin-6..  Journal of molecular biology,    (2):   [PMID:9159484]
24. Fishman, D D and 6 more authors..  (1998)  The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis..  The Journal of clinical investigation,    (1):   [PMID:9769329]
25. Foster, C B CB and 11 more authors..  (2000)  An IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in men infected with human immunodeficiency virus..  Blood,    (1):   [PMID:11001912]
26. Ota, N N and 8 more authors..  (2001)  A nucleotide variant in the promoter region of the interleukin-6 gene associated with decreased bone mineral density..  Journal of human genetics,      [PMID:11355017]
27. Chung, Hye Won HW and 8 more authors..  (2003)  Association of interleukin-6 promoter variant with bone mineral density in pre-menopausal women..  Journal of human genetics,      [PMID:12768442]
28. Nakahara, Hideko H and 6 more authors..  (2003)  Anti-interleukin-6 receptor antibody therapy reduces vascular endothelial growth factor production in rheumatoid arthritis..  Arthritis and rheumatism,      [PMID:12794819]
29. Nemeth, Elizabeta E and 6 more authors..  (2004)  IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin..  The Journal of clinical investigation,      [PMID:15124018]
30. Wolsk, Emil E, Mygind, Helene H, Grøndahl, Thomas S TS, Pedersen, Bente K BK and van Hall, Gerrit G..  (2010)  IL-6 selectively stimulates fat metabolism in human skeletal muscle..  American journal of physiology. Endocrinology and metabolism,      [PMID:20823453]
31. Ellingsgaard, Helga H and 20 more authors..  (2011)  Interleukin-6 enhances insulin secretion by increasing glucagon-like peptide-1 secretion from L cells and alpha cells..  Nature medicine,    (30):   [PMID:22037645]
32. Taniguchi, Koji K and 17 more authors..  (2015)  A gp130-Src-YAP module links inflammation to epithelial regeneration..  Nature,    (5):   [PMID:25731159]
33. Tagliabracci, Vincent S VS and 14 more authors..  (2015)  A Single Kinase Generates the Majority of the Secreted Phosphoproteome..  Cell,    (18):   [PMID:26091039]
34. Larsen, Jakob Vejby JV and Petersen, Claus Munck CM..  (2017)  SorLA in Interleukin-6 Signaling and Turnover..  Molecular and cellular biology,    (1):   [PMID:28265003]

Solution Calculators