Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
A cell-permeable isoxazoline compound that displays anti-inflammatory properties. Inhibits MIF dopachrome tautomerase activity by binding to its catalytic active site (IC50 = 7 µM for D-dopachrome tautomerase) and suppresses the production of TNFα, PGE2 and COX-2 in human monocytes and arachidonic acid in RAW 264.7 macrophages. Shown to exhibit antidiabetogenic properties in immunoinflammatory diabetic mouse model.
1.Lubetsky JB, Dios A, Han J, Aljabari B, Ruzsicska B, Mitchell R, Lolis E, Al-Abed Y. (2002) The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents.. J Biol Chem, 277 (28):(24976-82). [PMID:11997397][10.1021/op500134e]
2.Zapatero MC, Pérez P, Vázquez MJ, Colmenarejo G, de Los Frailes M, Ramón F. (2016) Discovery of Novel Inhibitors of the Tautomerase Activity of Macrophage Migration Inhibitory Factor (MIF).. J Biomol Screen, 21 (5):(446-58). [PMID:26933127][10.1021/op500134e]
3.Vujicic M, Nikolic I, Krajnovic T, Cheng KF, VanPatten S, He M, Stosic-Grujicic S, Stojanovic I, Al-Abed Y, Saksida T. (2014) Novel inhibitors of macrophage migration inhibitory factor prevent cytokine-induced beta cell death.. Eur J Pharmacol, 740 (13):(683-9). [PMID:24967533][10.1021/op500134e]
4.Cisneros JA, Robertson MJ, Valhondo M, Jorgensen WL. (2016) Irregularities in enzyme assays: The case of macrophage migration inhibitory factor.. Bioorg Med Chem Lett, 26 (12):(2764-2767). [PMID:27156768][10.1021/op500134e]
5.Bloom J, Metz C, Nalawade S, Casabar J, Cheng KF, He M, Sherry B, Coleman T, Forsthuber T, Al-Abed Y. (2016) Identification of Iguratimod as an Inhibitor of Macrophage Migration Inhibitory Factor (MIF) with Steroid-sparing Potential.. J Biol Chem, 291 (51):(26502-26514). [PMID:27793992][10.1021/op500134e]
6.Ito N et al.. (2020) Biphasic MIF and SDF1 expression during podocyte injury promote CD44-mediated glomerular parietal cell migration in focal segmental glomerulosclerosis.. Am J Physiol Renal Physiol, 318 (3):(F741-F753). [PMID:32068458]
7.Becker-Herman S et al.. (2021) CD74 is a regulator of hematopoietic stem cell maintenance.. PLoS Biol, 19 (3):(e3001121). [PMID:33661886]
8.Adzavon YM et al.. (2018) Macrophage migration inhibitory factor contributes to the pathogenesis of benign lymphoepithelial lesion of the lacrimal gland.. Cell Commun Signal, 16 (70). [PMID:30348174]
9.Yamamoto Y et al.. (2022) Neutrophils delay repair process in Wallerian degeneration by releasing NETs outside the parenchyma.. Life Sci Alliance, 5 (10):[PMID:35961782]
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