JC-1 - 95%, high purity , CAS No.3520-43-2

Item Number

J125134

• Synonyms: CBIC2 | JC1 | JC 1 | (E)-5,6-dichloro-2-(3-(5,6-dichloro-1,3-diethyl-1H-benzo[d]imidazol-2(3H)-ylidene)prop-1-en-1-yl)-1,3-diethyl-1H-benzo[d]imidazol-3-ium iodide | AS-73126 | 5,6-Dichloro-2-[(Z)-3-(5,6-dichloro-1,3-diethylbenzimidazol-3-ium-2-yl)prop-2-
• Specifications & Purity: ≥95%
• Storage Temp: Protected from light,Store at -20°C,Desiccated
• Shipped In: Ice chest + Ice pads
• Note: Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Product Description

JC-1 is a fluorescent lipophilic carbocyanine dye, which can be used to measure mitochondrial membrane potential.
A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition from red fluorescence to green fluorescence of JC-1 can also be used as an early detection indicator of apoptosis.

The maximum excitation wavelength of JC-1 monomer is 514nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-1 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence and green fluorescence.

The commonly used concentration range of JC-1 for detecting the mitochondrial membrane potential of cells is 1-20μg/mL, and the suitable concentration of JC-1 for many cells is 10μg/mL.

Precautions

If the amount of JC-1 used at a time is small, each tube needs to be appropriately divided to avoid repeated freeze-thaw.

For your safety and health, please wear lab coats and disposable gloves.