Basic Description

Product Name	JMJD6 Antibody - Primary antibody, specific to JMJD6, Rabbit IgG
Synonyms	EC 1.14.11.- \| Histone arginine demethylase JMJD6 \| JmjC domain-containing protein 6 \| Jumonji domain-containing protein 6 \| Lysyl-hydroxylase JMJD6 \| Peptide-lysine 5-dioxygenase JMJD6 \| Phosphatidylserine receptor \| Protein PTDSR \| Apoptotic cell cleara
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Rabbit
Specificity	JMJD6
Immunogen	Recombinant PSR expressed in E.coli (AA 1-334).
Positive Control	WB: K562, Hep G2 and HEK293 cell lysates.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Rabbit anti Human JMJD6 Antibody, Polyclonal, could be used for WB and so on. Application WB: 0.01-2µg/mL Function Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified + Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

JMJD6 Antibody (Ab111495) - Western Blot
All lanes: JMJD6 Antibody (Ab111495) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 49 kDa
Exposure time: 32.2 s

Associated Targets(Human)

JMJD6 Tchem Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (0 Activities)

Discover Target: JMJD6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

JMJD6 Tchem Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 (51 Activities)

Discover Target: JMJD6

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	0.01-2 µg/mL

Certificates

Certificate of Analysis(COA)

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404470	Certificate of Analysis	Apr 12, 2024	Ab111495
ZJ24F0404469	Certificate of Analysis	Apr 12, 2024	Ab111495
ZJ24F0404471	Certificate of Analysis	Apr 12, 2024	Ab111495

FAQs and Articles

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References

1. Chang B, Chen Y, Zhao Y, Bruick RK. (2007) JMJD6 is a histone arginine demethylase.. Science, 318 (5849): (444-7). [PMID:17947579]
2. Webby CJ, Wolf A, Gromak N, Dreger M, Kramer H, Kessler B, Nielsen ML, Schmitz C, Butler DS, Yates 3rd JR et al.. (2009) Jmjd6 catalyses lysyl-hydroxylation of U2AF65, a protein associated with RNA splicing.. Science, 325 (5936): (90-3). [PMID:19574390]
3. Pan S,Zhou Y,Wang Q,Wang Y,Tian C,Wang T,Huang L,Nan J,Li L,Yang S. (2020) Discovery and structure-activity relationship studies of 1-aryl-1H-naphtho[2,3-d][1,2,3]triazole-4,9-dione derivatives as potent dual inhibitors of indoleamine 2,3-dioxygenase 1 (IDO1) and trytophan 2,3-dioxygenase (TDO).. Eur J Med Chem, 207 (112703-112703). [PMID:32871341]
4. Nagase, T T and 6 more authors.. (1998) Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:9628581]
5. Cui, Ping P, Qin, Baoming B, Liu, Nan N, Pan, Guangjin G and Pei, Duanqing D.. (2004) Nuclear localization of the phosphatidylserine receptor protein via multiple nuclear localization signals.. Experimental cell research, (1): [PMID:14729065]
6. Cao, W M WM and 9 more authors.. (2004) Phosphatidylserine receptor cooperates with high-density lipoprotein receptor in recognition of apoptotic cells by thymic nurse cells.. Journal of molecular endocrinology, [PMID:15072554]
7. Köninger, Jörg J and 8 more authors.. (2005) Phosphatidylserine receptor in chronic pancreatitis: evidence for a macrophage independent role.. Annals of surgery, [PMID:15622002]
8. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196]
9. Hong, Xia X and 11 more authors.. (2010) Interaction of JMJD6 with single-stranded RNA.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:20679243]
10. Mantri, Monica M and 9 more authors.. (2010) Crystal structure of the 2-oxoglutarate- and Fe(II)-dependent lysyl hydroxylase JMJD6.. Journal of molecular biology, (13): [PMID:20684070]
11. Hahn, Phillip P and 9 more authors.. (2010) Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation.. PloS one, (29): [PMID:21060799]
12. Rahman, Shaila S and 6 more authors.. (2011) The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3.. Molecular and cellular biology, [PMID:21555454]
13. Han, Gang G and 6 more authors.. (2012) The hydroxylation activity of Jmjd6 is required for its homo-oligomerization.. Journal of cellular biochemistry, [PMID:22189873]
14. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
15. Liu, Wen W and 7 more authors.. (2013) Brd4 and JMJD6-associated anti-pause enhancers in regulation of transcriptional pause release.. Cell, (19): [PMID:24360279]
16. Poulard, Coralie C, Rambaud, Juliette J, Hussein, Nader N, Corbo, Laura L and Le Romancer, Muriel M.. (2014) JMJD6 regulates ERα methylation on arginine.. PloS one, [PMID:24498420]
17. Konuma, Tsuyoshi T and 8 more authors.. (2017) Structural Mechanism of the Oxygenase JMJD6 Recognition by the Extraterminal (ET) Domain of BRD4.. Scientific reports, (24): [PMID:29176719]
18. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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SKU	Size	Availability	Price
Ab111495-10μl	10μl	In stock	$69.90
Ab111495-50μl	50μl	In stock	$209.90
Ab111495-100μl	100μl	In stock	$309.90
Ab111495-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,799.90

JMJD6 Antibody - Primary antibody, specific to JMJD6, Rabbit IgG