Basic Description

Synonyms	Kanamycin sulfate \| 25389-94-0 \| Kanamycin A sulfate \| Kanamycin monosulfate \| Kanamycin acid sulfate \| Kanamycin (sulfate) \| Kanamycin A sulphate \| Ophtalmokalixan \| 133-92-6 \| Kanamycin Monosulphate \| 3847-27-6 \| J80EX28SMQ \| Kanamycin monosulfate [JAN] \| Kanamycin A monosulfate
Specifications & Purity	Animal Free
Biochemical and Physiological Mechanisms	Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding. Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can
Grade	Animal Free
Note	注意: Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.
Product Description	General Description Chemical structure: aminoglycoside Application Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus . It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL.

Associated Targets(Human)

HDAC6 Tclin Histone deacetylase 6 0 Activities

Discover Target: HDAC6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 0 Activities

Discover Target: TDP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Tyrosyl-DNA phosphodiesterase 1 345557 Activities

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

Histone deacetylase 6 222 Activities

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Streptococcus pneumoniae 31063 Activities

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Pseudomonas aeruginosa 123386 Activities

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Klebsiella pneumoniae 43867 Activities

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Proteus vulgaris 5823 Activities

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Micrococcus luteus 7463 Activities

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Staphylococcus aureus 210822 Activities

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Staphylococcus epidermidis 22802 Activities

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Escherichia coli 133304 Activities

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Proteus mirabilis 3894 Activities

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Salmonella 646 Activities

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Salmonella enteritidis 727 Activities

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Serratia marcescens 3237 Activities

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Streptococcus pyogenes 16140 Activities

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Bacillus subtilis 32866 Activities

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Mycobacterium tuberculosis 203094 Activities

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Enterococcus faecalis 29875 Activities

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Bacillus cereus 7522 Activities

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Candida albicans 78123 Activities

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Replicase polyprotein 1ab 378 Activities

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SARS-CoV-2 38078 Activities

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Names and Identifiers

IUPAC Name	(2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2R,3S,4R,6S)-4,6-diamino-3-[(2S,3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxyoxane-3,4,5-triol;sulfuric acid
INCHI	InChI=1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
InChi Key	OOYGSFOGFJDDHP-KMCOLRRFSA-N
Canonical SMILES	C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)O)O)OC3C(C(C(C(O3)CO)O)N)O)N.OS(=O)(=O)O
Isomeric SMILES	C1[C@H]([C@@H]([C@H]([C@@H]([C@H]1N)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CN)O)O)O)O)O[C@@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)N)O)N.OS(=O)(=O)O
RTECS	NZ3225030
Alternate CAS	70560-51-9
PubChem CID	441374
UN Number	2811
Packing Group	III
Molecular Weight	582.58
Beilstein	18(4)7631
Reaxy-Rn	3874279

Certificates

Certificate of Analysis(COA)

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Chemical and Physical Properties

Solubility	H2O: 50mg/mL, clear, colorless to faintly yellow
Specific Rotation[α]	117.5 ° (C=1, H2O)
Melt Point(°C)	250°C(dec.)(lit.)

Safety and Hazards(GHS)

Pictogram(s)	GHS08
Signal	Danger
Hazard Statements	H360:May damage fertility or the unborn child
Precautionary Statements	P280:Wear protective gloves/protective clothing/eye protection/face protection. P405:Store locked up. P501:Dispose of contents/container to ... P203:Obtain, read and follow all safety instructions before use. P318:if exposed or concerned, get medical advice.
RTECS	NZ3225030
Reaxy-Rn	3874279
Class	6.1
Merck Index	5281

Alternative Products

FAQs and Articles

FAQ

E. coli Competence Protocol for Plasmid Transformation

Categories: Protocols

Tags:

Antibiotics Microbiology Molecular Biology Plasmid transformation Vector construction E·coli competent cells Microbial medium raw materials Laboratory chemicals

Citations of This Product

1. Jingzhi Yang, Yami Ran, Shaopeng Liu, Chenhao Ren, Yuntian Lou, Pengfei Ju, Guoliang Li, Xiaogang Li, Dawei Zhang. (2023) Synergistic D-Amino Acids Based Antimicrobial Cocktails Formulated via High-Throughput Screening and Machine Learning. Advanced Science, 11 (9): (2307173). [PMID:38126652]
2. Jun Yao. (2024) A multiple signal amplification photoelectrochemical biosensor based on biotin-avidin system for kanamycin sensing in fish and milk via synergism of g-C3N4 and Ru@SiO2. ANALYTICA CHIMICA ACTA, 1288 (342141). [PMID:38220276]
3. Yin Bao, Kaiwei Cai, Yichen Liu, Bingling Li. (2023) Aptamer-Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip. CHINESE JOURNAL OF CHEMISTRY, (4): [PMID:16990800]
4. Pei Jia, Qiushuang Wu, Boyang Sun, Li Wang. (2023) Formic Acid-Regulated Defect Engineering in Zr-Based Metal-Organic Frameworks toward Fluorescence Sensor for Sensitive Detection of Chlortetracycline. Small, 19 (45): (2304096). [PMID:37415537]
5. Mingcong Rong, Yi Huang, Xiaoting Zhuang, Yingming Ma, Haijiao Xie, Yanfang Wu, Li Niu. (2023) AND logic-gate-based Au@MnO2 sensing platform for tetracyclines with fluorescent and colorimetric dual-signal readouts. SENSORS AND ACTUATORS B-CHEMICAL, 393 (134204). [PMID:]
6. Yang Zhenhua, Yang Xintong, Zhang Yuexia, Fan Xiaopeng, Cao Yujuan, Li Zhongping, Dong Chuan. (2023) Detection of oxytetracycline in milk using a novel carbon dots-based fluorescence probe via facile pyrolysis synthesis. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 30 (35): (84002-84010). [PMID:37353701]
7. Jianan Li, Wenjie Huang, Huixia He, Shuangni Shi, Xiuxia Sun, Jianxi Xiao. (2023) Biocompatible and bioactive hydrogels of recombinant fusion elastin with low transition temperature for improved healing of UV-irradiated skin. Journal of Materials Chemistry B, [PMID:37401183]
8. Ming Yi, Thi Dao Nguyen, Huan Liu, Yafang Liu, Shu Xiong, Yan Wang. (2023) A Boronate Ester Driven Rechargeable Antibacterial Membrane for Fast Molecular Sieving. ADVANCED FUNCTIONAL MATERIALS, 33 (26): (2213471). [PMID:]
9. Wanling Cui, Jinrong Liu, Wenqi Zhao, Jiayi Zhang, Yanyan Wang, Qiang Li, Rui Wang, Mei Qiao, Shicai Xu. (2023) An enzyme-free and label-free fluorescent aptasensor for sensitive detection of kanamycin in milk samples based on hybridization chain reaction. JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 120 (105300). [PMID:]
10. Zhen-Ping Zou, Yue Du, Ting-Ting Fang, Ying Zhou, Bang-Ce Ye. (2023) Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice. Cell Host & Microbe, 31 (199-212.e5). [PMID:36758520]
11. Baizhu Chen, Xiaoge Zhang, Lili Cheng, Xiaomei Chen, Junjie Tang, Peng Zhang, Chen Wang, Jie Liu. (2023) Surface programmed bacteria as photo-controlled NO generator for tumor immunological and gas therapy. JOURNAL OF CONTROLLED RELEASE, 353 (889). [PMID:36528194]
12. Jian Song, Kaiyu He, Bingcong Xing, Yong Pei, Dingnan Wang, Yang Wang, Shiyan Li, Jie Li, Weiwei Huan, Yiming Zhang, Bruce. D. Hammock. (2022) Rapid Measurement of Residual Kanamycin Using Highly Specific Biomimetic Recognition Paper-Based Chip. ANALYTICAL CHEMISTRY, 94 (50): (17567–17576). [PMID:36458677]
13. Qingbin Yuan, Pingfeng Yu, Yuan Cheng, Pengxiao Zuo, Yisi Xu, Yuxiao Cui, Yi Luo, Pedro J. J. Alvarez. (2022) Chlorination (but Not UV Disinfection) Generates Cell Debris that Increases Extracellular Antibiotic Resistance Gene Transfer via Proximal Adsorption to Recipients and Upregulated Transformation Genes. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 56 (23): (17166–17176). [PMID:36286344]
14. Ying Liu, Jingfeng Gao, Mingyan Zhao, Xiaoyu Fu, Yi Zhang, Haoran Zhang. (2023) Removal of antibiotic resistant bacteria, genes and inhibition of plasmid-mediated horizontal transfer by peroxymonosulfate: Efficiency and mechanisms. CHEMICAL ENGINEERING JOURNAL, 453 (139728). [PMID:]
15. Zishuo Zhang, Yuanyuan Wang, Ziyin Mei, Yiming Wang, Hui Li, Shaoguang Li, Fan Xia. (2022) Incorporating Hydrophobic Moieties into Self-Assembled Monolayers to Enable Electrochemical Aptamer-Based Sensors Deployed Directly in a Complex Matrix. ACS Sensors, 7 (9): (2615–2624). [PMID:35998663]
16. Yuhua Zhang, Qiaojun Fang, Hongfeng Wang, Jieyu Qi, Shan Sun, Menghui Liao, Yunhao Wu, Yangnan Hu, Pei Jiang, Cheng Cheng, Xiaoyun Qian, Mingliang Tang, Wei Cao, Shang Xiang, Chen Zhang, Jianming Yang, Xia Gao, Zheng Ying, Renjie Chai. (2023) Increased mitophagy protects cochlear hair cells from aminoglycoside-induced damage. Autophagy, 19 (1): (75-91). [PMID:35471096]
17. Wang Yang, Jin Daobin, Zhang Xiaoqian, Zhao Jikuan, Hun Xu. (2022) Self-enhancement photoelectrochemical strategy for kanamycin determination with amino functionalized MOFs. MICROCHIMICA ACTA, 189 (5): (1-7). [PMID:35426050]
18. Xuelian Hu, Ying Guo, Jinni Zhang, Xiaohui Wang, Guozhen Fang, Shuo Wang. (2022) A signal-amplified ratiometric fluorescence biomimetic sensor based on the synergistic effect of IFE and AE for the visual smart monitoring of oxytetracycline. CHEMICAL ENGINEERING JOURNAL, 433 (134499). [PMID:]
19. Zhou Li-Jian, Guo Li-Bin, Wei Wei, Lv Zhi-Xiang, Zhang Ye-Wang. (2021) A Novel Chondroitin AC Lyase With Broad Substrate Specificity From Pedobacter rhizosphaerae: Cloning, Expression, and Characterization. Frontiers in Bioengineering and Biotechnology, 9 (1336). [PMID:35004658]
20. Qi Nie, Jingjing Deng, Bing Xie, Guoyue Shi, Tianshu Zhou. (2021) A dual-channel colorimetric and fluorescent sensor for the rapid and ultrasensitive detection of kanamycin based on gold nanoparticles-copper nanoclusters. Analytical Methods, 13 (48): (5813-5820). [PMID:34852031]
21. Wanling Cui, Guodong Hu, Enguang Lv, Chonghui Li, Zhenxing Wang, Qiang Li, Zhaohong Qian, Jihua Wang, Shicai Xu, Rui Wang. (2022) A label-free and enzyme-free fluorescent aptasensor for amplified detection of kanamycin in milk sample based on target-triggered catalytic hairpin assembly. FOOD CONTROL, 133 (108654). [PMID:]

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Kanamycin sulfate from Streptomyces kanamyceticus - Animal Component-free, high purity , CAS No.25389-94-0