KAP1 Mouse mAb - Primary antibody, specific to KAP1, IgG1

    Application:
  • IF/ICC
  • IHC
  • IHC-F
  • IHC-P
  • IP
  • WB
Features and benefits
  • Host species: Mouse
  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG2b
  • Clone Number: 4E1-D12-F8
  • Conjugation: Unconjugated
Item Number
Ab111687
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab111687-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab111687-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab111687-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab111687-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,097.90

mAb(4E1-D12-F8); Mouse anti Human KAP1 Antibody; WB, IHC-F, IHC-P, ICC/IF, IP; Unconjugated

Basic Description

Product NameKAP1 Mouse mAb - Primary antibody, specific to KAP1, IgG1
SynonymsE3 SUMO protein ligase TRIM28 antibody | E3 SUMO-protein ligase TRIM28 antibody | FLJ29029 antibody | KAP 1 antibody | KAP-1 antibody | KRAB associated protein 1 antibody | KRAB interacting protein 1 antibody | KRAB-associated protein 1 antibody | KRAB-in
Specifications & PurityExactAb™, Validated, 1.0mg/mL
Host speciesMouse
SpecificityKAP1
ImmunogenPurified recombinant human KAP1/TIF1 beta protein fragments expressed in E.coli (AA 548-825).
Positive ControlWB: A549, Hep G2, HEK293, MCF7, HeLa, A431 lysates. IP: HeLa lysates. ICC/IF: HeLa cells. IHC: Human Spleen Tissue .
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Mouse anti Human KAP1 Antibody, 4E1-D12-F8, could be used for WB, IHC-F, IHC-P, ICC/IF, IP and so on.

Application:
WB: 1/500-1/1000
IHC-F: 1/50-1/100
IHC-P: 1/50-1/100
ICC/IF: 1/50-1/200
IP: 1/20

Protein Function
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes. Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.

Product Properties

IsotypeIgG1
Light Chain Typekappa
SDS-PAGE150kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0mg/mL
Storage TempStore at -20°C
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

TRIM28 Tbio Transcription intermediary factor 1-beta (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

">

1/500-1/1000

IF/ICC 1/50-1/200

">

1/50-1/200

IP

1/20

IHC-P

1/50-1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700725Certificate of AnalysisJul 31, 2023 Ab111687
ZJ23F0700724Certificate of AnalysisJul 31, 2023 Ab111687

Related Documents

References

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