Mouse anti Human Ki67 Antibody, Monoclonal (YN00996m), could be used for IHC and so on. Application IHC: 1/200 Protein Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Product Properties
Isotype
Mouse IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Ki67 Mouse mAb (Ab112020) - IHC Immunohistochemistry analysis of paraffin-embedded human tonsil tissue labelling Ki67 with Ki67 Mouse mAb (Ab112020) at 1/400 dilution. The section was incubated with Ki67 Mouse mAb (Ab112020) for 30 mins at room temperature. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.
Associated Targets(Human)
MKI67TbioProliferation marker protein Ki-67 (0 Activities)