ExactAb™, Validated, Carrier Free, High performance, Lot by Lot
Host species
Mouse
Specificity
KPNA2
Immunogen
Recombinant KPNa2 expressed in E.coli (AA 157-412).
Positive Control
WB: Jurkat, HEK293, HeLa, Hep G2, A549, NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Product Description
Mouse anti Human KPNA2 Antibody, Monoclonal (C8), could be used for WB, IHC and so on. Application: WB: 0.01-3µg/mL IHC : 5-20µg/mL Protein Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Product Properties
Isotype
Mouse IgG1
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
[Ab112361] KPNA2 Mouse mAb - Western Blot All lanes: KPNA2 Mouse mAb (Ab112361) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 63 kDa Exposure time: 6.6 s
KPNA2 Mouse mAb (Ab112361) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL KPNA2 Mouse mAb (Ab112361) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.