| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112438-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab112438-50μL | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab112438-100μL | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $327.90 | |
| Ab112438-1mL | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,297.90 |
mAb (5C5); Mouse anti Human Ku80 Antibody; WB, ELISA, IHC, Flow, IF, ICC; Unconjugated
| Product Name | Ku80 Mouse mAb - Primary antibody, specific to XRCC5, Mouse IgG1 |
|---|---|
| Synonyms | 86 kDa subunit of Ku antigen | ATP-dependent DNA helicase 2 subunit 2 | ATP-dependent DNA helicase II 80 kDa subunit | CTC box-binding factor 85 kDa subunit | CTC85 | CTCBF | DNA repair protein XRCC5 | EC 3.6.4.- | Ku80 | Ku86 | Lupus Ku autoantigen prote |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | XRCC5 |
| Immunogen | Purified recombinant fragment of human XRCC5 (corresponding to amino acids 61-340 of Human Ku80) expressed in E. Coli |
| Positive Control | WB: A549, HeLa, HEK293, K562 lysates. ICC/IF: HeLa cells. IHC: Human tonsil tissues, Human colon cancer tissue. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Application:
|
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid repeated freeze / thaw cycles. |
Ku80 Mouse mAb (Ab112438) - Western Blot
All lanes: Ku80 Mouse mAb (Ab112438) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Ku80 Mouse mAb (Ab112438) - ICC/IF
IF analysis of HeLa cells using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Ku80 Mouse mAb (Ab112438) - IHC
Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 with DAB staining.
Ku80 Mouse mAb (Ab112438) - IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 with DAB staining.
Ku80 Mouse mAb (Ab112438) - Flow Cytometry
Flow cytometric analysis of HeLa cells using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 (green) and negative control (purple).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
| IF/ICC |
1/200-1/1000
">
1/200-1/1000
|
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
">
1/10000
|
| IHC |
1/200-1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700541 | Certificate of Analysis | Jul 06, 2023 | Ab112438 |
| ZJ23F0700540 | Certificate of Analysis | Jul 06, 2023 | Ab112438 |
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| 2. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
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| 11. Chung, U U and 11 more authors.. (1996) The interaction between Ku antigen and REF1 protein mediates negative gene regulation by extracellular calcium.. The Journal of biological chemistry, (12): [PMID:8621488] |
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| 15. Zhang, Suisheng S, Schlott, Bernhard B, Görlach, Matthias M and Grosse, Frank F.. (2004) DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner.. Nucleic acids research, [PMID:14704337] |
| 16. Wang, Hong H, Fang, Ruihua R, Cho, Je-Yoel JY, Libermann, Towia A TA and Oettgen, Peter P.. (2004) Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86.. The Journal of biological chemistry, (11): [PMID:15075319] |
| 17. Gocke, Christian B CB, Yu, Hongtao H and Kang, Jungseog J.. (2005) Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates.. The Journal of biological chemistry, (11): [PMID:15561718] |
| 18. Falck, Jacob J, Coates, Julia J and Jackson, Stephen P SP.. (2005) Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage.. Nature, (31): [PMID:15758953] |
| 19. Iles, Natasha N, Rulten, Stuart S, El-Khamisy, Sherif F SF and Caldecott, Keith W KW.. (2007) APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks.. Molecular and cellular biology, [PMID:17353262] |
| 20. Kanno, Shin-ichiro S and 6 more authors.. (2007) A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses.. The EMBO journal, (18): [PMID:17396150] |
| 21. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 22. Roberts, Steven A SA and 6 more authors.. (2010) Ku is a 5'-dRP/AP lyase that excises nucleotide damage near broken ends.. Nature, (22): [PMID:20383123] |
| 23. Ahmad, Yasmeen; Boisvert, Francois-Michel; Lundberg, Emma; Uhlen, Mathias and Lamond, Angus I.. (2012) Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization.. Molecular & cellular proteomics : MCP, [PMID:22002106] |
| 24. Feng, Lin L and Chen, Junjie J.. (2012) The E3 ligase RNF8 regulates KU80 removal and NHEJ repair.. Nature structural & molecular biology, (22): [PMID:22266820] |
| 25. Jensik, Philip J PJ, Huggenvik, Jodi I JI and Collard, Michael W MW.. (2012) Deformed epidermal autoregulatory factor-1 (DEAF1) interacts with the Ku70 subunit of the DNA-dependent protein kinase complex.. PloS one, [PMID:22442688] |
| 26. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 27. Shirodkar, Purnata P, Fenton, Amanda L AL, Meng, Li L and Koch, C Anne CA.. (2013) Identification and functional characterization of a Ku-binding motif in aprataxin polynucleotide kinase/phosphatase-like factor (APLF).. The Journal of biological chemistry, (5): [PMID:23689425] |
| 28. Li, Pishun P and 7 more authors.. (2013) ABH2 couples regulation of ribosomal DNA transcription with DNA alkylation repair.. Cell reports, (29): [PMID:23972994] |
| 29. Beck, Carole C and 13 more authors.. (2014) PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways.. Nucleic acids research, [PMID:24598253] |
| 30. Slavoff, Sarah A SA, Heo, Jinho J, Budnik, Bogdan A BA, Hanakahi, Leslyn A LA and Saghatelian, Alan A.. (2014) A human short open reading frame (sORF)-encoded polypeptide that stimulates DNA end joining.. The Journal of biological chemistry, (18): [PMID:24610814] |
| 31. Ochi, Takashi T and 11 more authors.. (2015) DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.. Science (New York, N.Y.), (9): [PMID:25574025] |
| 32. Xing, Mengtan M and 18 more authors.. (2015) Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway.. Nature communications, (11): [PMID:25670504] |
| 33. Medunjanin, Senad S and 11 more authors.. (2015) DNA-dependent protein kinase (DNA-PK) permits vascular smooth muscle cell proliferation through phosphorylation of the orphan nuclear receptor NOR1.. Cardiovascular research, (1): [PMID:25852083] |
| 34. Craxton, A A and 5 more authors.. (2015) XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair.. Cell death and differentiation, [PMID:25941166] |
| 35. Nicolai, Serena S and 10 more authors.. (2015) Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics.. PloS one, [PMID:26030138] |
| 36. Kang, Ga-Young GY and 5 more authors.. (2015) Heat shock factor 1, an inhibitor of non-homologous end joining repair.. Oncotarget, (6): [PMID:26359349] |
| 37. Ismail, Ismail Hassan IH and 8 more authors.. (2015) The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice.. Nature cell biology, [PMID:26502055] |
| 38. Grundy, Gabrielle J GJ and 7 more authors.. (2016) The Ku-binding motif is a conserved module for recruitment and stimulation of non-homologous end-joining proteins.. Nature communications, (11): [PMID:27063109] |
| 39. Lescale, Chloé and 12 more authors.. (2016) Specific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination.. Cell reports, (13): [PMID:27601299] |
| 40. Morchikh, Mehdi M and 9 more authors.. (2017) HEXIM1 and NEAT1 Long Non-coding RNA Form a Multi-subunit Complex that Regulates DNA-Mediated Innate Immune Response.. Molecular cell, (3): [PMID:28712728] |
| 41. Arnoult, Nausica N and 10 more authors.. (2017) Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN.. Nature, (20): [PMID:28959974] |
| 42. Maekawa, Toshio and 11 more authors.. (2018) ATF7 mediates TNF-α-induced telomere shortening.. Nucleic acids research, (18): [PMID:29490055] |
| 43. Rushing, Amanda W AW, Hoang, Kimson K, Polakowski, Nicholas N and Lemasson, Isabelle I.. (2018) The Human T-Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor Attenuates Repair of Double-Stranded DNA Breaks via Nonhomologous End Joining.. Journal of virology, (1): [PMID:29769340] |
| 44. Shao, Zhengping and 13 more authors.. (2020) DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis.. Nature, [PMID:32103174] |
| 45. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
