| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112658-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab112658-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 | |
| Ab112658-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $259.90 | |
| Ab112658-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (C2); Mouse anti Human Lamin B1 Antibody; WB, IHC; Unconjugated
| Product Name | Lamin B1 Mouse mAb - Primary antibody, specific to LMNB1, Mouse IgG2b |
|---|---|
| Synonyms | ADLD antibody | lamin B1 antibody | Lamin-B1 antibody | LMN antibody | LMN2 antibody | LMNB antibody | Lmnb1 antibody | LMNB1_HUMAN antibody | MGC111419 antibody | OTTHUMP00000159218 antibody | ADLD | Lamin B1 | lamin-B1 | LMN | LMN2 | LMNB | LMNB1 | MGC1 |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA |
| Host species | Mouse |
| Specificity | LMNB1 |
| Immunogen | Recombinant LMNB1 expressed in E.coli (AA 52-233). |
| Positive Control | WB: MCF7, HeLa and Raw264.7 cell lysates. IHC: Human Kidney Tissue, Human Stomach Tissue, Human Ovarian cancer Tissue, Human Thyroid cancer Tissue, Human amy.gdalitis Tissue and Human Lung cancer Tissue. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human Lamin B1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A+G Affinity Chromatography |
| Form | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Lamin B1 Mouse mAb (Ab112658) - Western Blot
All lanes: Lamin B1 Mouse mAb (Ab112658) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 68 kDa
Exposure time: 30 s
Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mLHRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody .
Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Ovarian cancer Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Thyroid cancer Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human amy.gdalitis Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800962 | Certificate of Analysis | Sep 04, 2023 | Ab112658 |
| ZJ23F0800961 | Certificate of Analysis | Sep 04, 2023 | Ab112658 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 4. Pollard, K M KM and 5 more authors.. (1990) In vitro posttranslational modification of lamin B cloned from a human T-cell line.. Molecular and cellular biology, [PMID:2325650] |
| 5. Lin, F F and Worman, H J HJ.. (1995) Structural organization of the human gene (LMNB1) encoding nuclear lamin B1.. Genomics, (20): [PMID:7557986] |
| 6. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 7. Padiath, Quasar S QS and 8 more authors.. (2006) Lamin B1 duplications cause autosomal dominant leukodystrophy.. Nature genetics, [PMID:16951681] |
| 8. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 9. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 12. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 13. Ruan, Jianbin J and 7 more authors.. (2012) Crystal structures of the coil 2B fragment and the globular tail domain of human lamin B1.. FEBS letters, (17): [PMID:22265972] |
| 14. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 15. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 16. Johnson, Janel O JO and 41 more authors.. (2014) Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis.. Nature neuroscience, [PMID:24686783] |
| 17. Yeh, Chung-Hsin CH and 8 more authors.. (2015) SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells.. PloS one, [PMID:25775403] |
| 18. Cristofoli, Francesca and 19 more authors.. (2020) De Novo Variants in LMNB1 Cause Pronounced Syndromic Microcephaly and Disruption of Nuclear Envelope Integrity.. American journal of human genetics, (1): [PMID:32910914] |
| 19. Parry, David A and 17 more authors.. (2021) Heterozygous lamin B1 and lamin B2 variants cause primary microcephaly and define a novel laminopathy.. Genetics in medicine : official journal of the American College of Medical Genetics, [PMID:33033404] |
| 20. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
