mAb (J13); Mouse anti Human Lipocalin-2/NGAL Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab113231-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab113231-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab113231-50μl | 50μl | In stock | $189.90 | |
| Ab113231-100μl | 100μl | In stock | $299.90 | |
| Ab113231-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,019.90 |
mAb (J13); Mouse anti Human Lipocalin-2/NGAL Antibody; WB; Unconjugated
| Product Name | Lipocalin-2/NGAL Mouse mAb - Primary antibody, specific to LCN2, Mouse IgG1 |
|---|---|
| Synonyms | 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Onc |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | LCN2 |
| Immunogen | Recombinant NGALexpressed in E.coli (AA 21-198) |
| Positive Control | WB: HT-29, A431 and LNCaP cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human Lipocalin-2 / NGAL Antibody, Monoclonal (J13), could be used for WB and so on. Application: WB: 0.5-2µg/mL Protein Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Lipocalin-2/NGAL Mouse mAb (Ab113231) - Western Blot
All lanes: Lipocalin-2/NGAL Mouse mAb (Ab113231) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 12.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 0.5-2µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101837 | Certificate of Analysis | Nov 29, 2023 | Ab113231 |
| ZJ23F1101838 | Certificate of Analysis | Nov 29, 2023 | Ab113231 |
| 1. Guanhu Bao,Matthew Clifton,Trisha M Hoette,Kiyoshi Mori,Shi-Xian Deng,Andong Qiu,Melanie Viltard,David Williams,Neal Paragas,Thomas Leete,Ritwij Kulkarni,Xiangpo Li,Belinda Lee,Avtandil Kalandadze,Adam J Ratner,Juan Carlos Pizarro,Kai M Schmidt-Ott,Donald W Landry,Kenneth N Raymond,Roland K Strong,Jonathan Barasch. (2010-06-29) Iron traffics in circulation bound to a siderocalin (Ngal)-catechol complex.. Nature chemical biology, 6 ((8)): (602-609). [PMID:20581821] |
| 2. Triebel, S S, Bläser, J J, Reinke, H H and Tschesche, H H.. (1992) A 25 kDa alpha 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase.. FEBS letters, (21): [PMID:1281792] |
| 3. Kjeldsen, L L, Johnsen, A H AH, Sengeløv, H H and Borregaard, N N.. (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase.. The Journal of biological chemistry, (15): [PMID:7683678] |
| 4. Bartsch, S S and Tschesche, H H.. (1995) Cloning and expression of human neutrophil lipocalin cDNA derived from bone marrow and ovarian cancer cells.. FEBS letters, (9): [PMID:7835423] |
| 5. Bundgaard, J R JR, Sengeløv, H H, Borregaard, N N and Kjeldsen, L L.. (1994) Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils.. Biochemical and biophysical research communications, (15): [PMID:8060329] |
| 6. Cowland, J B JB and Borregaard, N N.. (1997) Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans.. Genomics, (1): [PMID:9339356] |
| 7. Coles, M M and 6 more authors.. (1999) The solution structure and dynamics of human neutrophil gelatinase-associated lipocalin.. Journal of molecular biology, (28): [PMID:10339412] |
| 8. Goetz, D H DH and 5 more authors.. (2000) Ligand preference inferred from the structure of neutrophil gelatinase associated lipocalin.. Biochemistry, (29): [PMID:10684642] |
| 9. Kristiansen, Troels Zakarias TZ and 8 more authors.. (2004) A proteomic analysis of human bile.. Molecular & cellular proteomics : MCP, [PMID:15084671] |
| 10. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053] |
| 11. Liu, Tao T and 6 more authors.. () Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 12. Ramachandran, Prasanna P and 5 more authors.. (2006) Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.. Journal of proteome research, [PMID:16740002] |
| 13. Sheng, Zhi Z, Wang, Shu-Zong SZ and Green, Michael R MR.. (2009) Transcription and signalling pathways involved in BCR-ABL-mediated misregulation of 24p3 and 24p3R.. The EMBO journal, (8): [PMID:19229297] |
| 14. Hoette, Trisha M and 5 more authors.. (2011) Immune interference in Mycobacterium tuberculosis intracellular iron acquisition through siderocalin recognition of carboxymycobactins.. ACS chemical biology, (16): [PMID:21978368] |
| 15. Allred, Benjamin E; Correnti, Colin; Clifton, Matthew C; Strong, Roland K and Raymond, Kenneth N.. (2013) Siderocalin outwits the coordination chemistry of vibriobactin, a siderophore of Vibrio cholerae.. ACS chemical biology, (20): [PMID:23755875] |
| 16. Shields-Cutler, Robin R RR and 5 more authors.. (2016) Human Metabolome-derived Cofactors Are Required for the Antibacterial Activity of Siderocalin in Urine.. The Journal of biological chemistry, (9): [PMID:27780864] |
| 17. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
