| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab113346-10μl | 10μl | In stock | $79.90 | |
| Ab113346-50μl | 50μl | In stock | $219.90 | |
| Ab113346-100μl | 100μl | In stock | $319.90 | |
| Ab113346-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
pAb; Rabbit anti Human LMAN1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | LMAN1 Antibody - Primary antibody, specific to LMAN1, Rabbit IgG |
|---|---|
| Synonyms | ER-Golgi intermediate compartment 53 kDa protein | Gp58 | Intracellular mannose-specific lectin MR60 | Lectin mannose-binding 1 | Endoplasmic reticulum golgi intermediate compartment protein 53antibody | ER-Golgi intermediate compartment 53 kDa protein an |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA |
| Host species | Rabbit |
| Specificity | LMAN1 |
| Immunogen | Recombinant LMAN1 expressed in E.coli (AA 198-475). |
| Positive Control | WB: HeLa, Hep G2, HEK293 and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human LMAN1 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purifie |
| Form | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
LMAN1 Antibody (Ab113346) - Western Blot
All lanes: LMAN1 Antibody (Ab113346) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 58 kDa
Observed band size: 53 kDa
Exposure time: 52.8 s
LMAN1 Antibody (Ab113346) - IF/ICC
FITC staining on IF
Sample: Human HeLa cell
Primary Ab: 20 µg/mL LMAN1 Antibody (Ab113346)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
LMAN1 Antibody (Ab113346) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 μg/mL LMAN1 Antibody (Ab113346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
| IF/ICC | 5-20µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Chart | Certificate Type | Date | Item |
|---|---|---|---|---|
 ZJ24F0404477 | Certificate of Analysis | Apr 12, 2024 | Ab113346 | |
| ZJ24F0404476 | Certificate of Analysis | Apr 12, 2024 | Ab113346 | |
| ZJ24F0404475 | Certificate of Analysis | Apr 12, 2024 | Ab113346 |
| 1. Arar, C C and 5 more authors.. (1995) ERGIC-53, a membrane protein of the endoplasmic reticulum-Golgi intermediate compartment, is identical to MR60, an intracellular mannose-specific lectin of myelomonocytic cells.. The Journal of biological chemistry, (24): [PMID:7876089] |
| 2. Fiedler, K K and Simons, K K.. (1994) A putative novel class of animal lectins in the secretory pathway homologous to leguminous lectins.. Cell, (3): [PMID:8205612] |
| 3. Schindler, R R, Itin, C C, Zerial, M M, Lottspeich, F F and Hauri, H P HP.. (1993) ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif.. European journal of cell biology, [PMID:8223692] |
| 4. Nichols, W C WC and 20 more authors.. (1999) ERGIC-53 gene structure and mutation analysis in 19 combined factors V and VIII deficiency families.. Blood, (1): [PMID:10090935] |
| 5. Harris, Robert A RA and 11 more authors.. (2002) Cluster analysis of an extensive human breast cancer cell line protein expression map database.. Proteomics, [PMID:11840567] |
| 6. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 7. Zhang, Bin B and 13 more authors.. (2003) Bleeding due to disruption of a cargo-specific ER-to-Golgi transport complex.. Nature genetics, [PMID:12717434] |
| 8. Cunningham, M A MA and 5 more authors.. (2003) LMAN1 is a molecular chaperone for the secretion of coagulation factor VIII.. Journal of thrombosis and haemostasis : JTH, [PMID:14629470] |
| 9. Neve, Etienne P A EP, Lahtinen, Ulla U and Pettersson, Ralf F RF.. (2005) Oligomerization and interacellular localization of the glycoprotein receptor ERGIC-53 is independent of disulfide bonds.. Journal of molecular biology, (2): [PMID:16257008] |
| 10. Yamada, Takayuki T and 9 more authors.. (2009) A novel missense mutation causing abnormal LMAN1 in a Japanese patient with combined deficiency of factor V and factor VIII.. American journal of hematology, [PMID:19787799] |
| 11. Wigren, Edvard E, Bourhis, Jean-Marie JM, Kursula, Inari I, Guy, Jodie E JE and Lindqvist, Ylva Y.. (2010) Crystal structure of the LMAN1-CRD/MCFD2 transport receptor complex provides insight into combined deficiency of factor V and factor VIII.. FEBS letters, (5): [PMID:20138881] |
| 12. Nishio, Miho M and 11 more authors.. (2010) Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency.. Proceedings of the National Academy of Sciences of the United States of America, (2): [PMID:20142513] |
| 13. Haines, Dale S DS and 8 more authors.. (2012) Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking.. Molecular & cellular proteomics : MCP, [PMID:22337587] |
| 14. Ong, Yan Shan YS, Tran, Ton Hoai Thi TH, Gounko, Natalia V NV and Hong, Wanjin W.. (2014) TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport.. Journal of cell science, (1): [PMID:24806965] |
| 15. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
