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LMAN1 Antibody - Primary antibody, specific to LMAN1, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefitsView More
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab113346
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab113346-10μl
10μl
10
$79.90
Ab113346-50μl
50μl
4
$219.90
Ab113346-100μl
100μl
2
$319.90
Ab113346-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,799.90

pAb; Rabbit anti Human LMAN1 Antibody; WB, IHC, IF, ICC; Unconjugated

AI Insight

Basic Description

Product NameLMAN1 Antibody - Primary antibody, specific to LMAN1, Rabbit IgG
SynonymsER-Golgi intermediate compartment 53 kDa protein | Gp58 | Intracellular mannose-specific lectin MR60 | Lectin mannose-binding 1 | Endoplasmic reticulum golgi intermediate compartment protein 53antibody | ER-Golgi intermediate compartment 53 kDa protein an
Specifications & PurityCarrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host speciesRabbit
SpecificityLMAN1
ImmunogenRecombinant LMAN1 expressed in E.coli (AA 198-475).
Positive ControlWB: HeLa, Hep G2, HEK293 and Jurkat cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Rabbit anti Human LMAN1 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.
Application
WB: 0.01-2µg/mL
IHC: 5-20µg/mL
IF/ICC: 5-20µg/mL
Function
Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Post-translational
The N-terminal may be partly blocked.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purifie
FormLiquid
ConcentrationSee COA
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

LMAN1 Antibody (Ab113346) - Western Blot
All lanes: LMAN1 Antibody (Ab113346) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 58 kDa
Observed band size: 53 kDa
Exposure time: 52.8 s

LMAN1 Antibody (Ab113346) - IF/ICC
FITC staining on IF
Sample: Human HeLa cell
Primary Ab: 20 µg/mL LMAN1 Antibody (Ab113346)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

LMAN1 Antibody (Ab113346) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 μg/mL LMAN1 Antibody (Ab113346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Associated Targets(Human)

LMAN1 Tbio Protein ERGIC-53 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC5-20 µg/mL

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5-20 µg/mL

WB 0.01-2 µg/mL

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0.01-2 µg/mL

IF/ICC

5-20µg/mL

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404477Certificate of AnalysisApr 12, 2024 Ab113346
ZJ24F0404476Certificate of AnalysisApr 12, 2024 Ab113346
ZJ24F0404475Certificate of AnalysisApr 12, 2024 Ab113346

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References

1. Arar, C C and 5 more authors..  (1995)  ERGIC-53, a membrane protein of the endoplasmic reticulum-Golgi intermediate compartment, is identical to MR60, an intracellular mannose-specific lectin of myelomonocytic cells..  The Journal of biological chemistry,    (24):   [PMID:7876089]
2. Fiedler, K K and Simons, K K..  (1994)  A putative novel class of animal lectins in the secretory pathway homologous to leguminous lectins..  Cell,    (3):   [PMID:8205612]
3. Schindler, R R, Itin, C C, Zerial, M M, Lottspeich, F F and Hauri, H P HP..  (1993)  ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif..  European journal of cell biology,      [PMID:8223692]
4. Nichols, W C WC and 20 more authors..  (1999)  ERGIC-53 gene structure and mutation analysis in 19 combined factors V and VIII deficiency families..  Blood,    (1):   [PMID:10090935]
5. Harris, Robert A RA and 11 more authors..  (2002)  Cluster analysis of an extensive human breast cancer cell line protein expression map database..  Proteomics,      [PMID:11840567]
6. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,      [PMID:12665801]
7. Zhang, Bin B and 13 more authors..  (2003)  Bleeding due to disruption of a cargo-specific ER-to-Golgi transport complex..  Nature genetics,      [PMID:12717434]
8. Cunningham, M A MA and 5 more authors..  (2003)  LMAN1 is a molecular chaperone for the secretion of coagulation factor VIII..  Journal of thrombosis and haemostasis : JTH,      [PMID:14629470]
9. Neve, Etienne P A EP, Lahtinen, Ulla U and Pettersson, Ralf F RF..  (2005)  Oligomerization and interacellular localization of the glycoprotein receptor ERGIC-53 is independent of disulfide bonds..  Journal of molecular biology,    (2):   [PMID:16257008]
10. Yamada, Takayuki T and 9 more authors..  (2009)  A novel missense mutation causing abnormal LMAN1 in a Japanese patient with combined deficiency of factor V and factor VIII..  American journal of hematology,      [PMID:19787799]
11. Wigren, Edvard E, Bourhis, Jean-Marie JM, Kursula, Inari I, Guy, Jodie E JE and Lindqvist, Ylva Y..  (2010)  Crystal structure of the LMAN1-CRD/MCFD2 transport receptor complex provides insight into combined deficiency of factor V and factor VIII..  FEBS letters,    (5):   [PMID:20138881]
12. Nishio, Miho M and 11 more authors..  (2010)  Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency..  Proceedings of the National Academy of Sciences of the United States of America,    (2):   [PMID:20142513]
13. Haines, Dale S DS and 8 more authors..  (2012)  Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking..  Molecular & cellular proteomics : MCP,      [PMID:22337587]
14. Ong, Yan Shan YS, Tran, Ton Hoai Thi TH, Gounko, Natalia V NV and Hong, Wanjin W..  (2014)  TMEM115 is an integral membrane protein of the Golgi complex involved in retrograde transport..  Journal of cell science,    (1):   [PMID:24806965]
15. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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