ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species
Mouse
Specificity
MECP2
Immunogen
Recombinant MECP2 expressed in E.coli (AA 1-177).
Positive Control
WB: SK-N-SH, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, Validated
Product Description
Mouse anti Human MeCP2 Antibody, Monoclonal (C7), could be used for WB and so on. Application: WB: 0.5-2µg/mL Protein Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Post-translational Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Product Properties
Isotype
Mouse IgG1
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8°C short term (2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
MeCP2 Mouse mAb (Ab114749) - Western Blot All lanes: MeCP2 Mouse mAb (Ab114749) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 52 kDa Observed band size: 81 kDa Exposure time: 60.0 s
MeCP2 Mouse mAb (Ab114749) - Western Blot All lanes: MeCP2 Mouse mAb (Ab114749) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 52 kDa Observed band size: 51 kDa Exposure time: 52.9 s
Associated Targets(Human)
MECP2Tchem Methyl-CpG-binding protein 2 0 Activities