| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab115157-10μl | 10μl | In stock | $69.90 | |
| Ab115157-50μl | 50μl | In stock | $199.90 | |
| Ab115157-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab115157-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,899.90 |
pAb; Rabbit anti Human METTL14 Antibody; WB, IHC; Unconjugated
| Product Name | METTL14 Antibody, specific to METTL14, Rabbit IgG |
|---|---|
| Synonyms | hMETTL14 | Methyltransferase-like protein 14 | METTL14 | hMETTL14 | KIAA1627 antibody | MET14_HUMAN antibody | Methyltransferase like 14 antibody | Methyltransferase-like protein 14 antibody | METTL14 antibody | N6-adenosine-methyltransferase subunit METT |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | METTL14 |
| Immunogen | A KLH conjugated synthetic peptide from the human region of human METTL14 (AA 2-36) |
| Positive Control | WB: A431, Jurkat, HeLa and HEK293 cell lysates. IHC: Human testis tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human METTL14 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified + Peptide affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
METTL14 Antibody (Ab115157) - Western Blot
All lanes: METTL14 Antibody (Ab115157) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa
Exposure time: 52.2 s
METTL14 Antibody (Ab115157) - IHC
METTL14 Antibody (Ab115157) staining METTL14 in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hour at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/1000
">
1/1000
|
| IHC | 1/250 "> 1/250 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404006 | Certificate of Analysis | Apr 01, 2024 | Ab115157 |
| ZJ24F0404005 | Certificate of Analysis | Apr 01, 2024 | Ab115157 |
| 1. Nagase T, Kikuno R, Nakayama M, Hirosawa M, Ohara O. (2000) Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.. DNA Res, 7 (4): (273-81). [PMID:10997877] [10.1021/op500134e] |
| 2. Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng X et al.. (2014) A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.. Nat Chem Biol, 10 (2): (93-5). [PMID:24316715] [10.1021/op500134e] |
| 3. Wang X, Feng J, Xue Y, Guan Z, Zhang D, Liu Z, Gong Z, Wang Q, Huang J, Tang C et al.. (2016) Structural basis of N(6)-adenosine methylation by the METTL3-METTL14 complex.. Nature, 534 (7608): (575-8). [PMID:27281194] [10.1021/op500134e] |
| 4. Xiong Y, Li F, Babault N, Dong A, Zeng H, Wu H, Chen X, Arrowsmith CH, Brown PJ, Liu J, Vedadi M, Jin J.. (2017) Discovery of Potent and Selective Inhibitors for G9a-Like Protein (GLP) Lysine Methyltransferase.. J Med Chem, 60 (5): (1876-1891). [PMID:28135087] [10.1021/acs.jmedchem.6b01645] |
| 5. Nakajima, Daisuke D and 5 more authors.. (2002) Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.. DNA research : an international journal for rapid publication of reports on genes and genomes, (30): [PMID:12168954] |
| 6. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 7. Ping, Xiao-Li XL and 24 more authors.. (2014) Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase.. Cell research, [PMID:24407421] |
| 8. Schwartz, Schraga S and 19 more authors.. (2014) Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites.. Cell reports, (10): [PMID:24981863] |
| 9. Liu, Nian N and 5 more authors.. (2015) N(6)-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions.. Nature, (26): [PMID:25719671] |
| 10. Zhou, Jun J and 5 more authors.. (2015) Dynamic m(6)A mRNA methylation directs translational control of heat shock response.. Nature, (22): [PMID:26458103] |
| 11. Wang, Ping P, Doxtader, Katelyn A KA and Nam, Yunsun Y.. (2016) Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases.. Molecular cell, (21): [PMID:27373337] |
| 12. Śledź, Paweł P and Jinek, Martin M.. (2016) Structural insights into the molecular mechanism of the m(6)A writer complex.. eLife, (14): [PMID:27627798] |
| 13. Schöller, Eva E and 8 more authors.. (2018) Interactions, localization, and phosphorylation of the m6A generating METTL3-METTL14-WTAP complex.. RNA (New York, N.Y.), [PMID:29348140] |
| 14. Yue, Yanan Y and 16 more authors.. (2018) VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation.. Cell discovery, [PMID:29507755] |
