pAb; Rabbit anti Human MGMT Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab115239-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab115239-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $774.90 | |
| Ab115239-50μl | 50μl | In stock | $199.90 | |
| Ab115239-100μl | 100μl | In stock | $289.90 | |
| Ab115239-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human MGMT Antibody; WB, IHC; Unconjugated
| Product Name | MGMT Antibody - Primary antibody, specific to MGMT, Rabbit IgG |
|---|---|
| Synonyms | 6 O methylguanine DNA methyltransferase antibody; 6-O-methylguanine-DNA methyltransferase antibody; Agat antibody; AGT antibody; AI267024 antibody; EC 2.1.1.63 antibody; Methylated DNA protein cysteine methyltransferase antibody; Methylated-DNA--protein-c |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Rabbit |
| Specificity | MGMT |
| Immunogen | Recombinant MGMT expressed in E.coli (AA 1-207). |
| Positive Control | WB: MCF7, Jurkat, HeLa, MOLT-4, HT-29 and Hep G2 cell lysate. IHC: Human stomach tissue. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human MGMT Antibody, Polyclonal antibody, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
MGMT Antibody (Ab115239) - Western Blot
All lanes: MGMT Antibody (Ab115239) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa
Exposure time: 2.6 s
MGMT Antibody (Ab115239) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10 µg/mL MGMT Antibody (Ab115239)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.5-3 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101587 | Certificate of Analysis | Nov 08, 2023 | Ab115239 |
| ZJ23F1101586 | Certificate of Analysis | Nov 08, 2023 | Ab115239 |
| 1. von Wronski, M A MA and 5 more authors.. (1991) Structural and immunological comparison of indigenous human O6-methylguanine-DNA methyltransferase with that encoded by a cloned cDNA.. The Journal of biological chemistry, (15): [PMID:1985934] |
| 2. Rydberg, B B, Spurr, N N and Karran, P P.. (1990) cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.. The Journal of biological chemistry, (5): [PMID:2188979] |
| 3. Hayakawa, H H, Koike, G G and Sekiguchi, M M.. (1990) Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA.. Journal of molecular biology, (20): [PMID:2359121] |
| 4. Koike, G G, Maki, H H, Takeya, H H, Hayakawa, H H and Sekiguchi, M M.. (1990) Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase.. The Journal of biological chemistry, (5): [PMID:2394694] |
| 5. Tano, K K, Shiota, S S, Collier, J J, Foote, R S RS and Mitra, S S.. (1990) Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2405387] |
| 6. Edara, S S, Goodtzova, K K and Pegg, A E AE.. (1995) The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity.. Carcinogenesis, [PMID:7614699] |
| 7. Kanugula, S S, Goodtzova, K K, Edara, S S and Pegg, A E AE.. (1995) Alteration of arginine-128 to alanine abolishes the ability of human O6-alkylguanine-DNA alkyltransferase to repair methylated DNA but has no effect on its reaction with O6-benzylguanine.. Biochemistry, (30): [PMID:7766621] |
| 8. Crone, T M TM, Goodtzova, K K, Edara, S S and Pegg, A E AE.. (1994) Mutations in human O6-alkylguanine-DNA alkyltransferase imparting resistance to O6-benzylguanine.. Cancer research, (1): [PMID:7954470] |
| 9. Liem, L K LK, Lim, A A and Li, B F BF.. (1994) Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA.. Nucleic acids research, (11): [PMID:8202360] |
| 10. Wibley, J E JE, Pegg, A E AE and Moody, P C PC.. (2000) Crystal structure of the human O(6)-alkylguanine-DNA alkyltransferase.. Nucleic acids research, (15): [PMID:10606635] |
| 11. Daniels, D S DS and 5 more authors.. (2000) Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding.. The EMBO journal, (3): [PMID:10747039] |
| 12. Rasimas, Joseph J JJ and 5 more authors.. (2003) Effects of zinc occupancy on human O6-alkylguanine-DNA alkyltransferase.. Biochemistry, (4): [PMID:12549918] |
| 13. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054] |
| 14. Duguid, Erica M EM, Rice, Phoebe A PA and He, Chuan C.. (2005) The structure of the human AGT protein bound to DNA and its implications for damage detection.. Journal of molecular biology, (22): [PMID:15964013] |
| 15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
