Basic Description

Product Name	Mre11 Mouse mAb - Primary antibody, specific to MRE11, IgG2b
Synonyms	AT like diseaseantibody; Ataxia telangiectasia disorder like antibody; ATLD antibody; DNA recombination and repair proteinantibody; Double strand break repair protein MRE11A antibody; Double-strand break repair protein MRE11A antibody; endo/exonuclease Mr
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0mg/mL
Host species	Mouse
Specificity	MRE11
Immunogen	Purified recombinant fragment of human MRE 11 (AA: 182-582) expressed in E. Coli.
Positive Control	WB: Hela, A431, MCF-7, Jurkat, HepG2, K562, COS-7, PC-12 and NIH/3T3 cell lysate. IHC: Ovarian cancer tissues; Rectal cancer tissues; Mouse testis tissues; Rat testis tissues; Rabbit testis tissues. Flow: Hela cells; K562 cells.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human Mre11 Antibody, Monoclonal antibody, could be used for ELISA, WB, Flow, IHC and so on. Application: WB: 1/500 - 1/2000. Predicted molecular weight: 80.6 kDa. IHC: 1/200 - 1/1000 Flow: 1/200 - 1/400 ELISA: 1/10000 Protein Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Post-translational Phosphorylated upon DNA damage, probably by ATM or ATR.

Product Properties

Isotype	IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	1.0mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Images

Mre11 Mouse mAb (Ab115912) - Western Blot
All lanes: Mre11 Mouse mAb (Ab115912) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 80.6 kDa
Observed band size: 84.2 kDa

Mre11 Mouse mAb (Ab115912) - Flow Cytometry
Flow cytometric analysis of cell line labeling Mre11 with Mre11 Mouse mAb (Ab115912) (green) compared with a negative control (red).

Mre11 Mouse mAb (Ab115912) - Flow Cytometry
Flow cytometric analysis of K562 cell line labeling Mre11 with Mre11 Mouse mAb (Ab115912) (green) compared with a negative control (red).

Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.

Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.

Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse testis tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.

Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded Rat testis tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.

Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded Rabbit testis tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.

Associated Targets

MRE11 Tbio Double-strand break repair protein MRE11 0 Activities

Discover Target: MRE11

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
Flow	1/200-1/400
ELISA	1/10000
IHC	1/200-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0800755	Certificate of Analysis	Aug 03, 2023	Ab115912
ZJ23F0800754	Certificate of Analysis	Aug 03, 2023	Ab115912

FAQs and Articles

References

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2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Huang F, Mazin AV.. (2014) Targeting the homologous recombination pathway by small molecule modulators.. Bioorg Med Chem Lett, 24 (14): (3006-3013). [PMID:24856061]
4. Petrini, J H JH and 5 more authors.. (1995) Isolation and characterization of the human MRE11 homologue.. Genomics, (1): [PMID:8530104]
5. Carney, J P JP and 8 more authors.. (1998) The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response.. Cell, (1): [PMID:9590181]
6. Paull, T T TT and Gellert, M M.. (1998) The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks.. Molecular cell, [PMID:9651580]
7. Trujillo, K M KM, Yuan, S S SS, Lee, E Y EY and Sung, P P.. (1998) Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95.. The Journal of biological chemistry, (21): [PMID:9705271]
8. Stewart, G S GS and 9 more authors.. (1999) The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia-telangiectasia-like disorder.. Cell, (10): [PMID:10612394]
9. Wang, Y Y and 5 more authors.. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.. Genes & development, (15): [PMID:10783165]
10. Zhao, S S and 13 more authors.. (2000) Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products.. Nature, (25): [PMID:10839544]
11. Zhu, X D XD, Küster, B B, Mann, M M, Petrini, J H JH and de Lange, T T.. (2000) Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres.. Nature genetics, [PMID:10888888]
12. Fukuda, T T and 9 more authors.. (2001) Alterations of the double-strand break repair gene MRE11 in cancer.. Cancer research, (1): [PMID:11196167]
13. Pitts, S A SA and 10 more authors.. (2001) hMRE11: genomic structure and a null mutation identified in a transcript protected from nonsense-mediated mRNA decay.. Human molecular genetics, (15): [PMID:11371508]
14. de Jager, M M and 5 more authors.. (2001) Human Rad50/Mre11 is a flexible complex that can tether DNA ends.. Molecular cell, [PMID:11741547]
15. Heikkinen, K K, Karppinen, S-M SM, Soini, Y Y, Mäkinen, M M and Winqvist, R R.. (2003) Mutation screening of Mre11 complex genes: indication of RAD50 involvement in breast and ovarian cancer susceptibility.. Journal of medical genetics, [PMID:14684699]
16. Lee, Ji-Hoon JH and Paull, Tanya T TT.. (2004) Direct activation of the ATM protein kinase by the Mre11/Rad50/Nbs1 complex.. Science (New York, N.Y.), (2): [PMID:15064416]
17. Zhang, Xiaoshan X and 5 more authors.. (2004) Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response.. Molecular and cellular biology, [PMID:15456891]
18. Chen, Ling L and 10 more authors.. (2005) Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage.. Cancer science, [PMID:15723659]
19. Bhoumik, Anindita A and 5 more authors.. (2005) ATM-dependent phosphorylation of ATF2 is required for the DNA damage response.. Molecular cell, (27): [PMID:15916964]
20. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
21. Bae, J-B JB and 9 more authors.. (2008) Snm1B/Apollo mediates replication fork collapse and S Phase checkpoint activation in response to DNA interstrand cross-links.. Oncogene, (28): [PMID:18469862]
22. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
23. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
24. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
25. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
26. Chaki, Moumita M and 72 more authors.. (2012) Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling.. Cell, (3): [PMID:22863007]
27. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
28. Lee, Kyung Yong KY and 6 more authors.. (2015) MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.. Nature communications, (28): [PMID:26215093]
29. Broderick, Ronan R and 7 more authors.. (2016) EXD2 promotes homologous recombination by facilitating DNA end resection.. Nature cell biology, [PMID:26807646]
30. Staples, Christopher J CJ and 12 more authors.. (2016) MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.. Cell reports, (6): [PMID:27568553]
31. Daddacha, Waaqo W and 27 more authors.. (2017) SAMHD1 Promotes DNA End Resection to Facilitate DNA Repair by Homologous Recombination.. Cell reports, (22): [PMID:28834754]
32. Coquel, Flavie F and 20 more authors.. (2018) SAMHD1 acts at stalled replication forks to prevent interferon induction.. Nature, [PMID:29670289]
33. Jachimowicz, Ron D RD and 30 more authors.. (2019) UBQLN4 Represses Homologous Recombination and Is Overexpressed in Aggressive Tumors.. Cell, (24): [PMID:30612738]

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SKU	Size	Availability	Price
Ab115912-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab115912-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$207.90
Ab115912-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$357.90
Ab115912-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$3,297.90

Mre11 Mouse mAb - Primary antibody, specific to MRE11, IgG2b