Protein Function: Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to posttranslational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.Tissue specificity: Ubiquitous; detected at low levels. RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Post-translational: Phosphorylated.
Product Properties
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
1.0mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Images
MSI2 Mouse mAb (Ab116122) - Western Blot All lanes: MSI2 Mouse mAb (Ab116122) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 35 kDa Observed band size: 35, 37 kDa Exposure time: 126.8 s
MSI2 Mouse mAb (Ab116122) - Western Blot All lanes: MSI2 Mouse mAb (Ab116122) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 35 kDa Observed band size: 35, 39 kDa Exposure time: 37.0 s
MSI2 Mouse mAb (Ab116122) - IHC Immunohistochemical analysis of paraffin-embedded ovarian cancer using MSI2 Mouse mAb (Ab116122) with DAB staining.
MSI2 Mouse mAb (Ab116122) - IHC Immunohistochemical analysis of cerebellum tissues using MSI2 Mouse mAb (Ab116122) with DAB staining.
Associated Targets(Human)
MSI2TbioRNA-binding protein Musashi homolog 2 (0 Activities)
1.Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)):(4493-4501). [PMID:19413330]
2.Barbouti, Aikaterini A and 7 more authors.. (2003) A novel gene, MSI2, encoding a putative RNA-binding protein is recurrently rearranged at disease progression of chronic myeloid leukemia and forms a fusion gene with HOXA9 as a result of the cryptic t(7;17)(p15;q23).. Cancer research, (15):[PMID:12649177]
3.Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8):[PMID:18691976]
4.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
5.Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.