MSI2 Mouse mAb - Primary antibody, specific to MSI2, Mouse IgG1

Features and benefits
  • Short Overview:

    mAb(2C11); Mouse anti Human MSI2 Antibody; WB, IHC, ELISA; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Mouse IgG1
    Application:
  • ELISA
  • IHC
  • WB
Item Number
Ab116122
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab116122-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab116122-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab116122-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90
Ab116122-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,967.90

mAb(2C11); Mouse anti Human MSI2 Antibody; WB, IHC, ELISA; Unconjugated

Basic Description

Product NameMSI2 Mouse mAb - Primary antibody, specific to MSI2, Mouse IgG1
SynonymsFLJ36569 antibody; MGC3245 antibody; Msi2 antibody; MSI2/HOXA9 fusion gene, included antibody; MSI2H antibody; MSI2H_HUMAN antibody; Musashi 2 antibody; Musashi homolog 2 antibody; Musashi RNA binding protein 2 antibody; Musashi, Drosophila, homolog of, 2
Specifications & PurityExactAb™, Validated, Carrier Free, 1.0mg/mL
Host speciesMouse
SpecificityMSI2
ImmunogenPurified recombinant fragment of human MSI2(AA: 1-190) expressed in E. Coli.
Positive ControlWB: HeLa, Hep G2 and A549 cell. IHC: Ovarian cancer tissues, Cerebellum tissues.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Mouse anti Human MSI2 Antibody, Monoclonal antibody, could be used for WB, IHC, ELISA and so on.

Application:
WB: 1/500 - 1/2000. Predicted molecular weight: 35 kDa.
IHC: 1/200-1/1000
ELISA: 1/10000


Protein Function:
Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to posttranslational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.Tissue specificity: Ubiquitous; detected at low levels.
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

Post-translational:
Phosphorylated.

Product Properties

IsotypeMouse IgG1
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageUpon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Images

MSI2 Mouse mAb (Ab116122) - Western Blot
All lanes: MSI2 Mouse mAb (Ab116122) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35, 37 kDa
Exposure time: 126.8 s


MSI2 Mouse mAb (Ab116122) - Western Blot
All lanes: MSI2 Mouse mAb (Ab116122) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 35, 39 kDa
Exposure time: 37.0 s

MSI2 Mouse mAb (Ab116122) - IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer using MSI2 Mouse mAb (Ab116122) with DAB staining.

MSI2 Mouse mAb (Ab116122) - IHC
Immunohistochemical analysis of cerebellum tissues using MSI2 Mouse mAb (Ab116122) with DAB staining.

Associated Targets(Human)

MSI2 Tbio RNA-binding protein Musashi homolog 2 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
ELISA

1/10000

IHC

1/200-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800773Certificate of AnalysisAug 08, 2023 Ab116122
ZJ23F0800774Certificate of AnalysisAug 08, 2023 Ab116122

Related Documents

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Barbouti, Aikaterini A and 7 more authors..  (2003)  A novel gene, MSI2, encoding a putative RNA-binding protein is recurrently rearranged at disease progression of chronic myeloid leukemia and forms a fusion gene with HOXA9 as a result of the cryptic t(7;17)(p15;q23)..  Cancer research,    (15):   [PMID:12649177]
3. Daub, Henrik H and 9 more authors..  (2008)  Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle..  Molecular cell,    (8):   [PMID:18691976]
4. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
5. Guo, Ailan and 15 more authors..  (2014)  Immunoaffinity enrichment and mass spectrometry analysis of protein methylation..  Molecular & cellular proteomics : MCP,      [PMID:24129315]

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