| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab116370-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $29.90 | |
| Ab116370-20μl | 20μl | In stock | $49.90 | |
| Ab116370-50μl | 50μl | In stock | $89.90 | |
| Ab116370-100μl | 100μl | In stock | $139.90 | |
| Ab116370-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,119.90 |
mAb (C1); Mouse anti Human MUC1 antibody; WB; Unconjugated
| Product Name | MUC1 Mouse mAb - Primary antibody, specific to MUC1, Mouse IgG1 |
|---|---|
| Synonyms | ADMCKD antibody | ADMCKD1 antibody | Breast carcinoma associated antigen DF3 antibody | Breast carcinoma-associated antigen DF3 antibody | CA 15-3 antibody | CA15 3 antibody | CA15 3 antigen antibody | CA15-3 antibody | CA15.3 antibody | Cancer antigen 15 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | MUC1 |
| Immunogen | Recombinant MUC1 expressed in E.coli (AA 1034~1151) |
| Positive Control | WB: Human milk. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Mouse anti Human MUC1 antibody; , Monoclonal (C1), could be used for WB and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G affinity purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
MUC1 Mouse mAb (Ab116370) - Western Blot
All lanes: MUC1 Mouse mAb (Ab116370) at 1/3000 dilution
Samples: Human milk
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 23,19 kDa
Exposure time: 10.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201971 | Certificate of Analysis | Dec 13, 2023 | Ab116370 |
| ZJ23F1201970 | Certificate of Analysis | Dec 13, 2023 | Ab116370 |
| ZJ23F1201969 | Certificate of Analysis | Dec 13, 2023 | Ab116370 |
| 1. Tsarfaty, I I and 9 more authors.. (1990) Isolation and characterization of an expressed hypervariable gene coding for a breast-cancer-associated antigen.. Gene, (14): [PMID:1688329] |
| 2. Gendler, S J SJ and 8 more authors.. (1990) Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin.. The Journal of biological chemistry, (5): [PMID:1697589] |
| 3. Hareuveni, M M and 9 more authors.. (1990) A transcribed gene, containing a variable number of tandem repeats, codes for a human epithelial tumor antigen. cDNA cloning, expression of the transfected gene and over-expression in breast cancer tissue.. European journal of biochemistry, (20): [PMID:2112460] |
| 4. Lancaster, C A CA and 5 more authors.. (1990) Structure and expression of the human polymorphic epithelial mucin gene: an expressed VNTR unit.. Biochemical and biophysical research communications, (31): [PMID:2268309] |
| 5. Ligtenberg, M J MJ, Vos, H L HL, Gennissen, A M AM and Hilkens, J J.. (1990) Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini.. The Journal of biological chemistry, (5): [PMID:2318825] |
| 6. Wreschner, D H DH and 9 more authors.. (1990) Human epithelial tumor antigen cDNA sequences. Differential splicing may generate multiple protein forms.. European journal of biochemistry, (20): [PMID:2351132] |
| 7. Lan, M S MS, Batra, S K SK, Qi, W N WN, Metzgar, R S RS and Hollingsworth, M A MA.. (1990) Cloning and sequencing of a human pancreatic tumor mucin cDNA.. The Journal of biological chemistry, (5): [PMID:2394722] |
| 8. Abe, M M, Siddiqui, J J and Kufe, D D.. (1989) Sequence analysis of the 5' region of the human DF3 breast carcinoma-associated antigen gene.. Biochemical and biophysical research communications, (15): [PMID:2597151] |
| 9. Gendler, S S, Taylor-Papadimitriou, J J, Duhig, T T, Rothbard, J J and Burchell, J J.. (1988) A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats.. The Journal of biological chemistry, (15): [PMID:3417635] |
| 10. Pandey, P P, Kharbanda, S S and Kufe, D D.. (1995) Association of the DF3/MUC1 breast cancer antigen with Grb2 and the Sos/Ras exchange protein.. Cancer research, (15): [PMID:7664271] |
| 11. Zrihan-Licht, S S and 7 more authors.. (1994) Characterization and molecular cloning of a novel MUC1 protein, devoid of tandem repeats, expressed in human breast cancer tissue.. European journal of biochemistry, (1): [PMID:7925397] |
| 12. Zrihan-Licht, S S, Baruch, A A, Elroy-Stein, O O, Keydar, I I and Wreschner, D H DH.. (1994) Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules.. FEBS letters, (12): [PMID:7988707] |
| 13. Yu, C J CJ and 8 more authors.. () Mucin mRNA expression in lung adenocarcinoma cell lines and tissues.. Oncology, [PMID:8604237] |
| 14. Weiss, M M, Baruch, A A, Keydar, I I and Wreschner, D H DH.. (1996) Preoperative diagnosis of thyroid papillary carcinoma by reverse transcriptase polymerase chain reaction of the MUC1 gene.. International journal of cancer, (28): [PMID:8608966] |
| 15. Yamamoto, M M, Bharti, A A, Li, Y Y and Kufe, D D.. (1997) Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion.. The Journal of biological chemistry, (9): [PMID:9139698] |
| 16. Oosterkamp, H M HM, Scheiner, L L, Stefanova, M C MC, Lloyd, K O KO and Finstad, C L CL.. (1997) Comparison of MUC-1 mucin expression in epithelial and non-epithelial cancer cell lines and demonstration of a new short variant form (MUC-1/Z).. International journal of cancer, (3): [PMID:9212228] |
| 17. Müller, S S and 5 more authors.. (1997) Localization of O-glycosylation sites on glycopeptide fragments from lactation-associated MUC1. All putative sites within the tandem repeat are glycosylation targets in vivo.. The Journal of biological chemistry, (3): [PMID:9312074] |
| 18. Li, Y Y, Bharti, A A, Chen, D D, Gong, J J and Kufe, D D.. (1998) Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin.. Molecular and cellular biology, [PMID:9819408] |
| 19. Baruch, A A and 10 more authors.. (1999) The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein.. Cancer research, (1): [PMID:10197628] |
| 20. Müller, S S and 5 more authors.. (1999) High density O-glycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells.. The Journal of biological chemistry, (25): [PMID:10373415] |
| 21. Li, Y Y, Kuwahara, H H, Ren, J J, Wen, G G and Kufe, D D.. (2001) The c-Src tyrosine kinase regulates signaling of the human DF3/MUC1 carcinoma-associated antigen with GSK3 beta and beta-catenin.. The Journal of biological chemistry, (2): [PMID:11152665] |
| 22. Parry, S S and 5 more authors.. (2001) Identification of MUC1 proteolytic cleavage sites in vivo.. Biochemical and biophysical research communications, (11): [PMID:11341784] |
| 23. Engelmann, K K, Baldus, S E SE and Hanisch, F G FG.. (2001) Identification and topology of variant sequences within individual repeat domains of the human epithelial tumor mucin MUC1.. The Journal of biological chemistry, (27): [PMID:11350974] |
| 24. Ren, Jian J, Li, Yongqing Y and Kufe, Donald D.. (2002) Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin signaling.. The Journal of biological chemistry, (17): [PMID:11877440] |
| 25. Thathiah, Amantha A, Blobel, Carl P CP and Carson, Daniel D DD.. (2003) Tumor necrosis factor-alpha converting enzyme/ADAM 17 mediates MUC1 shedding.. The Journal of biological chemistry, (31): [PMID:12441351] |
| 26. Li, Yongqing Y and 7 more authors.. (2003) Heregulin targets gamma-catenin to the nucleolus by a mechanism dependent on the DF3/MUC1 oncoprotein.. Molecular cancer research : MCR, [PMID:12939402] |
| 27. Huang, Lei L and 5 more authors.. () MUC1 cytoplasmic domain coactivates Wnt target gene transcription and confers transformation.. Cancer biology & therapy, [PMID:14688481] |
| 28. Kinlough, Carol L CL, Poland, Paul A PA, Bruns, James B JB, Harkleroad, Keri L KL and Hughey, Rebecca P RP.. (2004) MUC1 membrane trafficking is modulated by multiple interactions.. The Journal of biological chemistry, (17): [PMID:15471854] |
| 29. Mukherjee, P P, Tinder, T L TL, Basu, G D GD and Gendler, S J SJ.. (2005) MUC1 (CD227) interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells.. Journal of leukocyte biology, [PMID:15513966] |
| 30. Levitin, Fiana F and 19 more authors.. (2005) A novel protein derived from the MUC1 gene by alternative splicing and frameshifting.. The Journal of biological chemistry, (18): [PMID:15623537] |
| 31. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D.. (2005) Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.. Cancer cell, [PMID:15710329] |
| 32. Zhang, Li-Xin LX, Li, Chun-Hai CH, Sun, Li-Ya LY, Wang, Miao M and Lu, Hao-Jun HJ.. (2003) [Soluble expression of peptide containing MUC1/Y-specific epitope in Escherichia coli and preparation of the antibody].. Sheng wu gong cheng xue bao = Chinese journal of biotechnology, [PMID:15969018] |
| 33. Engelmann, Katja K and 6 more authors.. (2005) Transmembrane and secreted MUC1 probes show trafficking-dependent changes in O-glycan core profiles.. Glycobiology, [PMID:15972891] |
| 34. Levitin, Fiana F and 8 more authors.. (2005) The MUC1 SEA module is a self-cleaving domain.. The Journal of biological chemistry, (30): [PMID:15987679] |
| 35. Huang, Lei L and 5 more authors.. (2005) MUC1 oncoprotein blocks glycogen synthase kinase 3beta-mediated phosphorylation and degradation of beta-catenin.. Cancer research, (15): [PMID:16288032] |
| 36. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D.. (2006) MUC1 oncoprotein stabilizes and activates estrogen receptor alpha.. Molecular cell, (20): [PMID:16427018] |
| 37. Parry, Simon S and 6 more authors.. (2006) N-Glycosylation of the MUC1 mucin in epithelial cells and secretions.. Glycobiology, [PMID:16585136] |
| 38. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 39. Pochampalli, M R MR, el Bejjani, R M RM and Schroeder, J A JA.. (2007) MUC1 is a novel regulator of ErbB1 receptor trafficking.. Oncogene, (15): [PMID:16983337] |
| 40. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D.. (2007) Human mucin 1 oncoprotein represses transcription of the p53 tumor suppressor gene.. Cancer research, (15): [PMID:17308127] |
| 41. Lillehoj, Erik P EP, Lu, Wenju W, Kiser, Timothy T, Goldblum, Simeon E SE and Kim, K Chul KC.. (2007) MUC1 inhibits cell proliferation by a beta-catenin-dependent mechanism.. Biochimica et biophysica acta, [PMID:17524503] |
| 42. Singh, Pankaj K PK and 7 more authors.. (2007) Platelet-derived growth factor receptor beta-mediated phosphorylation of MUC1 enhances invasiveness in pancreatic adenocarcinoma cells.. Cancer research, (1): [PMID:17545600] |
| 43. Duffy, Michael J MJ, Evoy, Denis D and McDermott, Enda W EW.. (2010) CA 15-3: uses and limitation as a biomarker for breast cancer.. Clinica chimica acta; international journal of clinical chemistry, (14): [PMID:20816948] |
| 44. Zhang, Lixin L, Vlad, Anda A, Milcarek, Christine C and Finn, Olivera J OJ.. (2013) Human mucin MUC1 RNA undergoes different types of alternative splicing resulting in multiple isoforms.. Cancer immunology, immunotherapy : CII, [PMID:22941036] |
| 45. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 46. Kirby, Andrew A and 42 more authors.. (2013) Mutations causing medullary cystic kidney disease type 1 lie in a large VNTR in MUC1 missed by massively parallel sequencing.. Nature genetics, [PMID:23396133] |
| 47. Li, Y Y and 7 more authors.. (2001) The epidermal growth factor receptor regulates interaction of the human DF3/MUC1 carcinoma antigen with c-Src and beta-catenin.. The Journal of biological chemistry, (21): [PMID:11483589] |
