Nemolizumab (anti-IL-31Ra) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to IL31RA, >95%, high purity, Human IgG2, Antibody of Interleukin-31 receptor; α subunit

    Application:
  • IF/ICC
Features and benefits
  • Host species: Human
  • Species reactivity(Reacts with): Cynomolgus monkey,Human
  • Isotype: Human IgG2
  • Conjugation: Unconjugated
Item Number
Ab170532
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab170532-100μg
100μg
In stock
$39.90
Ab170532-1mg
1mg
In stock
$177.90
Ab170532-5mg
5mg
In stock
$447.90
Ab170532-10mg
10mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$747.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG2; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

Basic Description

Product NameNemolizumab (anti-IL-31Ra) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to IL31RA, >95%, high purity, Human IgG2
SynonymsCytokine receptor-like 3 | GLM-R | Gp130-like monocyte receptor | Gp130-like receptor | hGLM-R | IL-31 receptor subunit alpha | IL-31R subunit alpha | IL-31RA | IL-31R-alpha | ZcytoR17
Specifications & PurityExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
Host speciesHuman
SpecificityIL31RA
ApplicationELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action TypeINHIBITOR
Mechanism of actionAntibody of Interleukin-31 receptor; α subunit
Product Description

Nemolizumab (anti-IL-31Ra) is a humanized antihuman interleukin-31 receptor A monoclonal antibody that inhibits the binding of interleukin-31 (IL-31) to its receptor and subsequent signal transduction. Nemolizumab can used be in research of atopic dermatitis (AD).
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Product Properties

Antibody TypePrimary antibody
ClonalityRecombinant
IsotypeHuman IgG2
Light Chain Typekappa
SDS-PAGE26.5 kDa (Light Chain) & 54.0 kDa (Heavy Chain), under reducing conditions; 175.4 kDa, under non-reducing conditions.
Purification MethodProtein A purified
Purity>95%
SourceCHO supernatant
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -80°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS1476039-58-3

Images

Nemolizumab (anti-IL-31Ra) (Ab170532) - SEC
The purity of Nemolizumab (anti-IL-31Ra) (Ab170532) is more than 95% verified by HPLC.

Nemolizumab (anti-IL-31Ra) (Ab170532) - ELISA
Immobilized Human IL-31RA - Fc tag at 2 μg/mL can bind Nemolizumab (anti-IL-31Ra) (Ab170532) with the EC₅₀ of 20.79 ng/mL.

Associated Targets(Human)

IL31RA Tbio Interleukin-31 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IF/ICC 1/50-1/200

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1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303672Certificate of AnalysisMar 25, 2024 Ab170532
ZJ24F0303671Certificate of AnalysisMar 25, 2024 Ab170532
ZJ24F0303670Certificate of AnalysisMar 25, 2024 Ab170532

Related Documents

References

1. Kasutani K, Fujii E, Ohyama S, Adachi H, Hasegawa M, Kitamura H, Yamashita N.  (2014)  Anti-IL-31 receptor antibody is shown to be a potential therapeutic option for treating itch and dermatitis in mice..  Br J Pharmacol,  171  (22): (5049-58).  [PMID:24946165] [10.1021/op500134e]
2. Kabashima K, Matsumura T, Komazaki H, Kawashima M, Nemolizumab-JP01 Study Group.  (2020)  Trial of Nemolizumab and Topical Agents for Atopic Dermatitis with Pruritus..  N Engl J Med,  383  (2): (141-150).  [PMID:32640132] [10.1021/op500134e]
3. Kabashima K, Furue M, Hanifin JM, Pulka G, Wollenberg A, Galus R, Etoh T, Mihara R, Nakano M, Ruzicka T.  (2018)  Nemolizumab in patients with moderate-to-severe atopic dermatitis: Randomized, phase II, long-term extension study..  J Allergy Clin Immunol,  142  (4): (1121-1130.e7).  [PMID:29753033] [10.1021/op500134e]
4. Nemoto O, Furue M, Nakagawa H, Shiramoto M, Hanada R, Matsuki S, Imayama S, Kato M, Hasebe I, Taira K et al..  (2016)  The first trial of CIM331, a humanized antihuman interleukin-31 receptor A antibody, in healthy volunteers and patients with atopic dermatitis to evaluate safety, tolerability and pharmacokinetics of a single dose in a randomized, double-blind, placebo-controlled study..  Br J Dermatol,  174  (2): (296-304).  [PMID:26409172] [10.1021/op500134e]
5. Dillon SR, Sprecher C, Hammond A, Bilsborough J, Rosenfeld-Franklin M, Presnell SR, Haugen HS, Maurer M, Harder B, Johnston J et al..  (2004)  Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice..  Nat Immunol,  (7): (752-60).  [PMID:15184896] [10.1021/op500134e]
6. Ghilardi, Nico N and 5 more authors..  (2002)  A novel type I cytokine receptor is expressed on monocytes, signals proliferation, and activates STAT-3 and STAT-5..  The Journal of biological chemistry,    (10):   [PMID:11877449]
7. Diveu, Caroline C and 14 more authors..  (2003)  GPL, a novel cytokine receptor related to GP130 and leukemia inhibitory factor receptor..  The Journal of biological chemistry,    (12):   [PMID:14504285]
8. Dreuw, Alexandra A and 5 more authors..  (2004)  Characterization of the signaling capacities of the novel gp130-like cytokine receptor..  The Journal of biological chemistry,    (20):   [PMID:15194700]
9. Diveu, Caroline C and 8 more authors..  ()  Predominant expression of the long isoform of GP130-like (GPL) receptor is required for interleukin-31 signaling..  European cytokine network,      [PMID:15627637]
10. Bilsborough, Janine J and 10 more authors..  (2006)  IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis..  The Journal of allergy and clinical immunology,      [PMID:16461143]
11. Jawa, Randeep S RS and 6 more authors..  (2008)  Regulated expression of the IL-31 receptor in bronchial and alveolar epithelial cells, pulmonary fibroblasts, and pulmonary macrophages..  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research,      [PMID:18439099]
12. Lin, Ming-Wei MW and 16 more authors..  (2010)  Novel IL31RA gene mutation and ancestral OSMR mutant allele in familial primary cutaneous amyloidosis..  European journal of human genetics : EJHG,      [PMID:19690585]
13. Kasraie, Sadaf S, Niebuhr, M M, Baumert, K K and Werfel, T T..  (2011)  Functional effects of interleukin 31 in human primary keratinocytes..  Allergy,      [PMID:21261663]
14. Cevikbas, Ferda F and 17 more authors..  (2014)  A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1..  The Journal of allergy and clinical immunology,      [PMID:24373353]

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