Nepetin - 99%, high purity , CAS No.520-11-6

Item Number

N649844

• Synonyms: 5,7,3',4'-TETRAHYDROXY-6-METHOXY FLAVONE | EUPAFOLIN | NCGC00163594-01 | REGID_for_CID_5317284 | SR-01000777526-3 | cid_5317284 | DTXSID8061509 | p-hydroxy benzyl alcohol | ?6-METHOXYLUTEOLIN | 4H-1-Benzopyran-4-one, 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-
• Specifications & Purity: ≥98%
• Biochemical and Physiological Mechanisms: Nepetin (6-Methoxyluteolin) is a natural flavonoid isolated from Eupatorium ballotaefolium HBK with potent anti-inflammatory activities. Nepetin inhibits IL-6 , IL-8 and MCP-1 secretion with IC 50 values of 4.43 μM, 3.42 μM and 4.17 μM, respectively in AR
• Storage Temp: Protected from light,Store at -20°C,Desiccated
• Shipped In: Ice chest + Ice pads

Product Description

Nepetin (6-Methoxyluteolin) is a natural flavonoid isolated from Eupatorium ballotaefolium HBK with potent anti-inflammatory activities. Nepetin inhibits IL-6 , IL-8 and MCP-1 secretion with IC 50 values of 4.43 μM, 3.42 μM and 4.17 μM, respectively in ARPE-19 cells

In Vitro

Pretreatment with Nepetin dose-dependently inhibited IL-6, IL-8 and MCP-1 secretion with IC 50 values of 4.43, 3.42 and 4.17 μM, respectively. Nepetin (2.5-10 μM; 25 hours; ARPE-19 cells) treatment suppresses IL-1β-induced cytokine (IL-6, IL-8 and MCP-1) expression at mRNA level in ARPE-19 cells. Nepetin (2.5-10 μM; 1.5 hours; ARPE-19 cells) treatment dose-dependently inhibits phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Nepetin decreases the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. RT-PCRCell Line: ARPE-19 cells Concentration: 2.5 μM, 5 μM, 10 μM Incubation Time: 25 hours Result: Suppressed the mRNA expression of IL-6, IL-8 and MCP-1 in ARPE-19 cells. Western Blot AnalysisCell Line: ARPE-19 cells Concentration: 2.5 μM, 5 μM, 10 μM Incubation Time: 1.5 hours Result: Dose-dependently inhibited phosphorylation of IKKα/β and IκBα, and nuclear translocation of p65. Decreased the level of phosphorylated ERK1/2, JNK and p38 MAPK in activated ARPE-19 cells.

Form:Solid

IC50& Target:IL-1 IL-6 IL-8