| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab118002-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $36.90 | |
| Ab118002-50μl | 50μl | In stock | $99.90 | |
| Ab118002-100μl | 100μl | In stock | $149.90 | |
| Ab118002-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $999.90 |
mAb(C2); Mouse anti Human NGF Antibody; WB; Unconjugated
| Product Name | NGF Mouse mAb - Primary antibody, specific to NGF , Mouse IgG1 |
|---|---|
| Synonyms | Beta nerve growth factor precursor | beta-nerve growth factor | betaNGF | beta-NGF | HSAN5 | MGC161426 | nerve growth factor (beta polypeptide) | nerve growth factor, beta subunit | NGFB | NGFBMGC161428 | Beta nerve growth factor antibody | Beta NGF antib |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA |
| Host species | Mouse |
| Specificity | NGF |
| Immunogen | Recombinant NGF expressed in E.coli (AA 19-239) |
| Positive Control | WB: HeLa, Jurkat, A549 and HEK293 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human NGF Antibody, Monoclonal(C2), could be used for WB and so on . Application: WB: 0.01-2 µg/mL Protein Function Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (4 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
NGF Mouse mAb (Ab118002) - Western Blot
All lanes: NGF Mouse mAb (Ab118002) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 31 kDa
Exposure time: 109.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101688 | Certificate of Analysis | Nov 16, 2023 | Ab118002 |
| ZJ23F1101689 | Certificate of Analysis | Nov 16, 2023 | Ab118002 |
| 1. Klein, R R, Jing, S Q SQ, Nanduri, V V, O'Rourke, E E and Barbacid, M M.. (1991) The trk proto-oncogene encodes a receptor for nerve growth factor.. Cell, (5): [PMID:1849459] |
| 2. Hallböök, F F, Ibáñez, C F CF and Persson, H H.. (1991) Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary.. Neuron, [PMID:2025430] |
| 3. Borsani, G G and 7 more authors.. (1990) cDNA sequence of human beta-NGF.. Nucleic acids research, (11): [PMID:2374737] |
| 4. Ullrich, A A, Gray, A A, Berman, C C, Coussens, L L and Dull, T J TJ.. (1983) Sequence homology of human and mouse beta-NGF subunit genes.. Cold Spring Harbor symposia on quantitative biology, [PMID:6327169] |
| 5. Ullrich, A A, Gray, A A, Berman, C C and Dull, T J TJ.. (1983) Human beta-nerve growth factor gene sequence highly homologous to that of mouse.. Nature, (30): [PMID:6688123] |
| 6. Tong, Y Y, Wang, H H and Chen, W W.. (1997) [Cloning and sequencing of the gene for premature beta nerve growth factor].. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, [PMID:10322959] |
| 7. Cargill, M M and 17 more authors.. (1999) Characterization of single-nucleotide polymorphisms in coding regions of human genes.. Nature genetics, [PMID:10391209] |
| 8. Culmsee, C C, Semkova, I I and Krieglstein, J J.. (1999) NGF mediates the neuroprotective effect of the beta2-adrenoceptor agonist clenbuterol in vitro and in vivo: evidence from an NGF-antisense study.. Neurochemistry international, [PMID:10403429] |
| 9. Wiesmann, C C, Ultsch, M H MH, Bass, S H SH and de Vos, A M AM.. (1999) Crystal structure of nerve growth factor in complex with the ligand-binding domain of the TrkA receptor.. Nature, (9): [PMID:10490030] |
| 10. Semkova, I I and Krieglstein, J J.. (1999) Neuroprotection mediated via neurotrophic factors and induction of neurotrophic factors.. Brain research. Brain research reviews, [PMID:10525174] |
| 11. Puls, I I and 5 more authors.. (1999) [Clenbuterol in amyotrophic lateral sclerosis. No indication for a positive effect].. Der Nervenarzt, [PMID:10637819] |
| 12. Riaz, S S SS and Tomlinson, D R DR.. (1999) Clenbuterol stimulates neurotrophic support in streptozotocin-diabetic rats.. Diabetes, obesity & metabolism, [PMID:11221812] |
| 13. Einarsdottir, Elisabet E and 8 more authors.. (2004) A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.. Human molecular genetics, (15): [PMID:14976160] |
| 14. He, Xiao-Lin XL and Garcia, K Christopher KC.. (2004) Structure of nerve growth factor complexed with the shared neurotrophin receptor p75.. Science (New York, N.Y.), (7): [PMID:15131306] |
| 15. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 16. Wehrman, Tom T and 5 more authors.. (2007) Structural and mechanistic insights into nerve growth factor interactions with the TrkA and p75 receptors.. Neuron, (4): [PMID:17196528] |
| 17. Wijeyewickrema, Lakshmi C LC, Gardiner, Elizabeth E EE, Gladigau, Elsa L EL, Berndt, Michael C MC and Andrews, Robert K RK.. (2010) Nerve growth factor inhibits metalloproteinase-disintegrins and blocks ectodomain shedding of platelet glycoprotein VI.. The Journal of biological chemistry, (16): [PMID:20164177] |
| 18. Carvalho, Ofélia P OP and 8 more authors.. (2011) A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy.. Journal of medical genetics, [PMID:20978020] |
| 19. Davidson, G L G and 20 more authors.. (2012) Frequency of mutations in the genes associated with hereditary sensory and autonomic neuropathy in a UK cohort.. Journal of neurology, [PMID:22302274] |
| 20. Cao, Ziqin and 6 more authors.. (2020) Targeting nerve growth factor, a new option for treatment of osteoarthritis: a network meta-analysis of comparative efficacy and safety with traditional drugs.. Aging, (3): [PMID:33293475] |
