Click Here for 5% Off Your First Aladdin Purchase!
Call +1 (833) 552-7181 or Contact us Earn $25 in coupons on your first order with Aladdin!
Toggle Nav
My Cart
Search
Advanced Search
Menu
Sign In
྾
main product photo

Nile Red - suitable for fluorescence, ≥95.0% (HPLC), high purity , CAS No.7385-67-3

  • BioReagent
  • for fluorescence analysis
  • ≥95%(HPLC)
Item Number
N121291
Grouped product items
SKUSizeAvailabilityPrice Qty
N121291-250mg
250mg
In stock
$89.90
N121291-1g
1g
In stock
$239.90
N121291-5g
5g
In stock
$839.90
Add to Wish List Compare
Bulk Order  SDS  DATASHEET

Discover Nile Red by Aladdin Scientific in suitable for fluorescence, ≥95.0% (HPLC) for only $99.90. Available - in Ligands at Aladdin Scientific. Photostable, lipophilic stain Tags: Fluorescent indicators and probes, Six-Membered Heterocyclic Compounds.

View related series
Fluorescent indicators and probes Six-Membered Heterocyclic Compounds

Basic Description

SynonymsNile red|7385-67-3|9-(diethylamino)-5H-benzo[a]phenoxazin-5-one|Nile Blue A Oxazone|9-(diethylamino)benzo[a]phenoxazin-5-one|Phenoxazone 9|C20H18N2O2|Nile Blue A oxazone;Phenoxazone 9|MFCD00011639|MLS001164197|CHEBI:52169|9-(Diethylamino)-5H-benzo(a)pheno
Specifications & Puritysuitable for fluorescence, ≥95.0% (HPLC)
Storage TempStore at 2-8°C,Argon charged
Shipped InWet ice
GradeBioReagent, for fluorescence analysis
Product Description

Nile Red is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments. This lipophilic stain is commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile Red has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Nile Red's fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites.
A photostable, lipophilic stain with bright red fluorescence

Names and Identifiers

IUPAC Name 9-(diethylamino)benzo[a]phenoxazin-5-one
INCHI InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChi Key VOFUROIFQGPCGE-UHFFFAOYSA-N
Canonical SMILES CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2
Isomeric SMILES CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2
WGK Germany 3
PubChem CID 65182
Molecular Weight 318.37
Beilstein 279110
Reaxy-Rn 279110

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

15 results found

Lot NumberCertificate TypeDateItem
G2408311Certificate of AnalysisJun 24, 2024 N121291
G2408316Certificate of AnalysisJun 24, 2024 N121291
D2418277Certificate of AnalysisMar 26, 2024 N121291
D2418488Certificate of AnalysisMar 26, 2024 N121291
D2418492Certificate of AnalysisMar 26, 2024 N121291
D2418493Certificate of AnalysisMar 26, 2024 N121291
L2315295Certificate of AnalysisNov 22, 2023 N121291
L2315296Certificate of AnalysisNov 22, 2023 N121291
L2315298Certificate of AnalysisNov 22, 2023 N121291
B2302578Certificate of AnalysisJan 09, 2023 N121291
B2302579Certificate of AnalysisJan 09, 2023 N121291
B2302580Certificate of AnalysisJan 09, 2023 N121291
A2219023Certificate of AnalysisJan 04, 2022 N121291
A2219024Certificate of AnalysisJan 04, 2022 N121291
A2219025Certificate of AnalysisJan 04, 2022 N121291

more

Chemical and Physical Properties

SolubilitySoluble in water (Partly miscible), and methanol (1 mg/ml).
Sensitivitylight sensitive
Melt Point(°C)203-205°C

Safety and Hazards(GHS)

WGK Germany 3
Reaxy-Rn 279110

Related Documents

 SDS

 Specification Sheet

References

1. Lattao R et al..  (2021)  Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins..  Dev Cell,      [PMID:33725482]
2. P Greenspan,E P Mayer,S D Fowler.  (1985-03-01)  Nile red: a selective fluorescent stain for intracellular lipid droplets..  The Journal of cell biology,  100  ((3)): (965-973).  [PMID:3972906]
3. Ryan B Smit,Ralf Schnabel,Jeb Gaudet.  (2008-10-18)  The HLH-6 transcription factor regulates C. elegans pharyngeal gland development and function..  PLoS genetics,  4  ((10)): (e1000222-e1000222).  [PMID:18927627]
4. Kevin B Vargo,Ranganath Parthasarathy,Daniel A Hammer.  (2012-07-04)  Self-assembly of tunable protein suprastructures from recombinant oleosin..  Proceedings of the National Academy of Sciences of the United States of America,  109  ((29)): (11657-11662).  [PMID:22753512]
5. Robert D Gardner,Egan Lohman,Robin Gerlach,Keith E Cooksey,Brent M Peyton.  (2012-07-07)  Comparison of CO(2) and bicarbonate as inorganic carbon sources for triacylglycerol and starch accumulation in Chlamydomonas reinhardtii..  Biotechnology and bioengineering,  110  ((1)): (87-96).  [PMID:22767335]
6. Feyza Gunduz,Fatma M Aboulnasr,Partha K Chandra,Sidhartha Hazari,Bret Poat,Darren P Baker,Luis A Balart,Srikanta Dash.  (2012-08-07)  Free fatty acids induce ER stress and block antiviral activity of interferon alpha against hepatitis C virus in cell culture..  Virology journal,  9  (143-143).  [PMID:22863531]
7. Ni Zeng,Xiaoling Gao,Quanyin Hu,Qingxiang Song,Huimin Xia,Zhongyang Liu,Guangzhi Gu,Mengyin Jiang,Zhiqing Pang,Hongzhuan Chen,Jun Chen,Liang Fang.  (2012-08-14)  Lipid-based liquid crystalline nanoparticles as oral drug delivery vehicles for poorly water-soluble drugs: cellular interaction and in vivo absorption..  International journal of nanomedicine,  7  (3703-3718).  [PMID:22888230]
8. Minnie Chan,Eric Schopf,Jagadis Sankaranarayanan,Adah Almutairi.  (2012-08-16)  Iron oxide nanoparticle-based magnetic resonance method to monitor release kinetics from polymeric particles with high resolution..  Analytical chemistry,  84  ((18)): (7779-7784).  [PMID:22891894]
9. Wantong Song,Zhaohui Tang,Mingqiang Li,Shixian Lv,Haiyang Yu,Lili Ma,Xiuli Zhuang,Yubin Huang,Xuesi Chen.  (2012-08-29)  Tunable pH-sensitive poly(β-amino ester)s synthesized from primary amines and diacrylates for intracellular drug delivery..  Macromolecular bioscience,  12  ((10)): (1375-1383).  [PMID:22927338]
10. Kazuhito Tanabe,Takumi Asada,Sei-ichi Nishimoto.  (2012-10-23)  Aggregate formation and radiolytic degradation of amphiphilic DNA block copolymer possessing disulfide bond..  Bioorganic & medicinal chemistry letters,  22  ((23)): (7045-7047).  [PMID:23084897]
11. Mehrnaz Azami-Movahed,Sajad Shariatizi,Marjan Sabbaghian,Atiyeh Ghasemi,Azadeh Ebrahim-Habibi,Mohsen Nemat-Gorgani.  (2012-10-24)  Heme binding site in apomyoglobin may be effectively targeted with small molecules to control aggregation..  The international journal of biochemistry & cell biology,  45  ((2)): (299-307).  [PMID:23089873]
12. Guo-Dong Feng,Fang Zhang,Li-Hua Cheng,Xin-Hua Xu,Lin Zhang,Huan-Lin Chen.  (2012-12-01)  Evaluation of FT-IR and Nile Red methods for microalgal lipid characterization and biomass composition determination..  Bioresource technology,  128  (107-112).  [PMID:23196229]
13. Ulf Maeder,Kay Marquardt,Sebastian Beer,Thorsten Bergmann,Thomas Schmidts,Johannes T Heverhagen,Klemens Zink,Frank Runkel,Martin Fiebich.  (2012-12-12)  Evaluation and quantification of spectral information in tissue by confocal microscopy..  Journal of biomedical optics,  17  ((10)): (106011-106011).  [PMID:23224010]
14. Nobuo Uehara,Osamu Yoshida.  (2012-12-13)  Release of Nile red from thermoresponsive gold nanocomposites by heating a solution and the addition of glutathione..  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,  28  ((12)): (1125-1132).  [PMID:23232230]
15. Daisuke Horita,Hiroaki Todo,Kenji Sugibayashi.  (2014-06-03)  Analysis of the pretreatment effect of ethanol on the stratum corneum- and hair follicular-penetration of drugs using the hair follicle-plugging method..  Chemical & pharmaceutical bulletin,  62  ((6)): (578-585).  [PMID:24881664]
16. J P Neves Silva,I M S C Oliveira,A C N Oliveira,M Lúcio,A C Gomes,P J G Coutinho,M E C D Real Oliveira.  (2014-07-01)  Structural dynamics and physicochemical properties of pDNA/DODAB:MO lipoplexes: effect of pH and anionic lipids in inverted non-lamellar phases versus lamellar phases..  Biochimica et biophysica acta,  1838  ((10)): (2555-2567).  [PMID:24976292]
17. Claire Levrier,Martin C Sadowski,Colleen C Nelson,Peter C Healy,Rohan A Davis.  (2015-01-13)  Denhaminols A-H, dihydro-β-agarofurans from the endemic Australian rainforest plant Denhamia celastroides..  Journal of natural products,  78  ((1)): (111-119).  [PMID:25579619]
18. Ruiying Song,Na Du,Xiaoyu Zhu,Haiping Li,Shue Song,Wanguo Hou.  (2015-02-17)  Rough glass surface-mediated transition of micelle-to-vesicle in sodium dodecylbenzenesulfonate solutions..  The journal of physical chemistry. B,  119  ((9)): (3762-3767).  [PMID:25686019]
19. Karel A Erion,Charles A Berdan,Nathan E Burritt,Barbara E Corkey,Jude T Deeney.  (2015-05-03)  Chronic Exposure to Excess Nutrients Left-shifts the Concentration Dependence of Glucose-stimulated Insulin Secretion in Pancreatic β-Cells..  The Journal of biological chemistry,  290  ((26)): (16191-16201).  [PMID:25934392]
20. Centdrika R Dates,Tariq Fahmi,Sebastian J Pyrek,Aiwei Yao-Borengasser,Barbara Borowa-Mazgaj,Stacie M Bratton,Susan A Kadlubar,Peter I Mackenzie,Randy S Haun,Anna Radominska-Pandya.  (2015-05-23)  Human UDP-Glucuronosyltransferases: Effects of altered expression in breast and pancreatic cancer cell lines..  Cancer biology & therapy,  16  ((5)): (714-723).  [PMID:25996841]
21. Albert Herms,Marta Bosch,Babu J N Reddy,Nicole L Schieber,Alba Fajardo,Celia Rupérez,Andrea Fernández-Vidal,Charles Ferguson,Carles Rentero,Francesc Tebar,Carlos Enrich,Robert G Parton,Steven P Gross,Albert Pol.  (2015-05-28)  AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation..  Nature communications,  6  (7176-7176).  [PMID:26013497]
22. James A Levitt,Pei-Hua Chung,Klaus Suhling.  (2015-09-04)  Spectrally resolved fluorescence lifetime imaging of Nile red for measurements of intracellular polarity..  Journal of biomedical optics,  20  ((9)): (096002-096002).  [PMID:26334975]
23. Nausika Betriu,Claire Jarrosson-Moral,Carlos E Semino.  (2020-05-02)  Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold..  Biomolecules,  10  ((5)):   [PMID:32354097]
24. Ruimin Sun,Xiaohui Kang,Yan Zhao,Zhanyu Wang,Ruiwen Wang,Rong Fu,Yang Li,Yan Hu,Zhecheng Wang,Wen Shan,Junjun Zhou,Xiaofeng Tian,Jihong Yao.  (2020-06-11)  Sirtuin 3-mediated deacetylation of acyl-CoA synthetase family member 3 by protocatechuic acid attenuates non-alcoholic fatty liver disease..  British journal of pharmacology,  177  ((18)): (4166-4180).  [PMID:32520409]
25. Manuel Quiñones-Pérez,Ruben J Cieza,Bryan Khai D Ngo,Melissa A Grunlan,Maribella Domenech.  (2020-12-04)  Amphiphilic silicones to reduce the absorption of small hydrophobic molecules..  Acta biomaterialia,  121  (339-348).  [PMID:33271355]
26. Fernando Ezquer,Marcelo Ezquer,Valeska Simon,Paulette Conget.  (2011-02-10)  The antidiabetic effect of MSCs is not impaired by insulin prophylaxis and is not improved by a second dose of cells..  PloS one,  6  ((1)): (e16566-e16566).  [PMID:21304603]
27. Zhen Zhang,Xiaoqin Xiong,Jiangling Wan,Ling Xiao,Lu Gan,Youmei Feng,Huibi Xu,Xiangliang Yang.  (2012-07-17)  Cellular uptake and intracellular trafficking of PEG-b-PLA polymeric micelles..  Biomaterials,  33  ((29)): (7233-7240).  [PMID:22795850]
28. Michitaka Suzuki,Yuki Shinohara,Toyoshi Fujimoto.  (2012-10-03)  Histochemical detection of lipid droplets in cultured cells..  Methods in molecular biology (Clifton, N.J.),  931  (483-491).  [PMID:23027019]
29. Yaroslav V Faletrov,Nina S Frolova,Hanna V Hlushko,Elena V Rudaya,Irina P Edimecheva,Stephan Mauersberger,Vladimir M Shkumatov.  (2013-04-05)  Evaluation of the fluorescent probes Nile Red and 25-NBD-cholesterol as substrates for steroid-converting oxidoreductases using pure enzymes and microorganisms..  The FEBS journal,  280  ((13)): (3109-3119).  [PMID:23551929]

Solution Calculators

Privacy Policy Website Terms of Use Return policy Terms and Conditions of Sale Cookie Policy Sales Policy
Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.
Copyright © 2023-present Aladdin Scientific Corp . All rights reserved.