Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product (diketohydrindylidene-diketohydrindamine). Ninhydrin is commonly used for the development of fingermarks on porous surfaces and is often used in comparison with
Storage Temp
Store at 2-8°C,Argon charged,Desiccated
Shipped In
Wet ice
Grade
ACS
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines. Reaction with sarcosine or proline gives azomethine ylides.
Application：
It can be used for the detection of peptides, amino acids, amino acids and amino sugars, and can produce strong fluorescent ternary compounds with aldehydes and primary amines. Reacts with sarcosine or proline to form methylimine Ylide. Ninhydrin (ninhydrin hydrate) can be used as a reagent for the determination of amino acids and peptides by photometry and colorimetry. It can also be used to prepare ninhydrin reagents by mixing ninhydrin with hydroninhydrin (reduced form of ninhydrin) in dimethyl sulfoxide. This reagent can be used for the manual ninhydrin method to quantify amino acids. It can be used to detect free amino groups in amino acids, peptides and proteins.
F2417304
