Chromogenic cephalosporin β-lactamase substrate. Indicates β-lactamase activity via a red color intensely proportional to the original concentration of Nitrocefin.
Storage Temp
Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Nitrocefin is a chromogenic β-lactamase substrate that undergoes colour change from yellow to red as the amide bond in the β-Lactam ring is hydrolyzed by β-lactamase. Nitrocefin undergoes colour changes induced by lactamases produced by Gram-positive and Gram-negative bacteria. Several studies have utilized the colour changing properties of Nitrocefin for the detection of β-lactamase activity from bacterial cell extracts by isoelectric focusing and spectroscopy. Nitrocefin has also been used in studies involving β-lactamase resistant antibiotics.
1.Aparna Vasudevan, Dinesh Kumar Kesavan, Liang Wu, Zhaoliang Su, Shengjun Wang, Mohan Kumar Ramasamy, Waheeta Hopper, Huaxi Xu. (2022) In Silico and In Vitro Screening of Natural Compounds as Broad-Spectrum β-Lactamase Inhibitors against Acinetobacter baumannii New Delhi Metallo-β-lactamase-1 (NDM-1). Biomed Research International, 2022 (4230788). [PMID:35372567][10.1155/2022/4230788]
3.Maohu Chen, Bo Qiu, Zhanlin Zhang, Shuang Xie, Yuan Liu, Tian Xia, Xiaohong Li. (2021) Light-triggerable and pH/lipase-responsive release of antibiotics and β-lactamase inhibitors from host-guest self-assembled micelles to combat biofilms and resistant bacteria. CHEMICAL ENGINEERING JOURNAL, 424 (130330). [PMID:][10.1016/j.cej.2021.130330]
4.Dongmei Zhao, Hongru Li, Chengcheng Yue, Kaili Sun, Yuanyuan Dai, Hui Zhang, Yanyan Liu, Yufeng Gao, Jiabin Li. (2021) Captopril potentiated meropenem activity against MBL-producing carbapenem-resistant Klebsiella pneumoniae: in vitro and in vivo study. JOURNAL OF INORGANIC BIOCHEMISTRY, 218 (111381). [PMID:33647540][10.1016/j.jinorgbio.2021.111381]
5.Minrui Liu, Hongyuhang Ni, Ling Yang, Gang Chen, Xiaojun Yan, Xiaoyun Leng, Pu Liu, Xiangkai Li. (2019) Pretreatment of swine manure containing β-lactam antibiotics with whole-cell biocatalyst to improve biogas production. Journal of Cleaner Production, 240 (118070). [PMID:][10.1016/j.jclepro.2019.118070]
References
1.Gardner TJ et al.. (2022) Engineering CAR-T cells to activate small-molecule drugs in situ.. Nat Chem Biol, 18 (2):(216-225). [PMID:34969970]
2.Aparna Vasudevan, Dinesh Kumar Kesavan, Liang Wu, Zhaoliang Su, Shengjun Wang, Mohan Kumar Ramasamy, Waheeta Hopper, Huaxi Xu. (2022) In Silico and In Vitro Screening of Natural Compounds as Broad-Spectrum β-Lactamase Inhibitors against Acinetobacter baumannii New Delhi Metallo-β-lactamase-1 (NDM-1). Biomed Research International, 2022 (4230788). [PMID:35372567][10.1155/2022/4230788]
4.Maohu Chen, Bo Qiu, Zhanlin Zhang, Shuang Xie, Yuan Liu, Tian Xia, Xiaohong Li. (2021) Light-triggerable and pH/lipase-responsive release of antibiotics and β-lactamase inhibitors from host-guest self-assembled micelles to combat biofilms and resistant bacteria. CHEMICAL ENGINEERING JOURNAL, 424 (130330). [PMID:][10.1016/j.cej.2021.130330]
5.Dongmei Zhao, Hongru Li, Chengcheng Yue, Kaili Sun, Yuanyuan Dai, Hui Zhang, Yanyan Liu, Yufeng Gao, Jiabin Li. (2021) Captopril potentiated meropenem activity against MBL-producing carbapenem-resistant Klebsiella pneumoniae: in vitro and in vivo study. JOURNAL OF INORGANIC BIOCHEMISTRY, 218 (111381). [PMID:33647540][10.1016/j.jinorgbio.2021.111381]
6.Minrui Liu, Hongyuhang Ni, Ling Yang, Gang Chen, Xiaojun Yan, Xiaoyun Leng, Pu Liu, Xiangkai Li. (2019) Pretreatment of swine manure containing β-lactam antibiotics with whole-cell biocatalyst to improve biogas production. Journal of Cleaner Production, 240 (118070). [PMID:][10.1016/j.jclepro.2019.118070]
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