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Nitrotetrazolium Blue chloride - for molecular biology,98%, high purity , CAS No.298-83-9

  • for molecular biology
  • ≥98%
Item Number
N104910
Grouped product items
SKUSizeAvailabilityPrice Qty
N104910-50mg
50mg
In stock
$11.90
N104910-250mg
250mg
In stock
$46.90
N104910-1g
1g
In stock
$141.90
N104910-5g
5g
In stock
$636.90
N104910-25g
25g
In stock
$2,866.90
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Chromogenic substrate.

View related series
Cell signal transduction and Neuroscience Detecting substrates and enzymes oxidative stress

Basic Description

Synonyms298-83-9|Nitrotetrazolium Blue chloride|Nitroblue tetrazolium chloride|nitro bt|Nitro Blue tetrazolium chloride|Nitro Blue tetrazolium|NBT|Nitrotetrazolium Blue|p-Nitrotetrazolium Blue|p-Nitro Blue tetrazolium chloride|p-Nitroblue Tetrazolium Chloride|MFC
Specifications & Purityfor molecular biology, ≥98%
Biochemical and Physiological MechanismsChromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase.
Storage TempStore at 2-8°C,Argon charged
Shipped InWet ice
Gradefor molecular biology
NoteWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

Substrate for dehydrogenases and other oxidases

Names and Identifiers

IUPAC Name 2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyltetrazol-2-ium-2-yl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium;dichloride
INCHI InChI=1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChi Key FSVCQIDHPKZJSO-UHFFFAOYSA-L
Canonical SMILES COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8.[Cl-].[Cl-]
Isomeric SMILES COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8.[Cl-].[Cl-]
WGK Germany 3
RTECS XF8045000
PubChem CID 9281
Molecular Weight 817.6
Beilstein 4115923
Reaxy-Rn 3896823

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

17 results found

Lot NumberCertificate TypeDateItem
G23151116Certificate of AnalysisJun 26, 2023 N104910
H2405018Certificate of AnalysisJun 26, 2023 N104910
G2404068Certificate of AnalysisJun 26, 2023 N104910
G23151122Certificate of AnalysisJun 26, 2023 N104910
G23151119Certificate of AnalysisJun 26, 2023 N104910
G23151118Certificate of AnalysisJun 26, 2023 N104910
G23151117Certificate of AnalysisJun 26, 2023 N104910
G23151115Certificate of AnalysisJun 26, 2023 N104910
G23151111Certificate of AnalysisJun 26, 2023 N104910
G23151110Certificate of AnalysisJun 26, 2023 N104910
F2217278Certificate of AnalysisJun 23, 2022 N104910
F2217276Certificate of AnalysisJun 20, 2022 N104910
F2217275Certificate of AnalysisJun 20, 2022 N104910
H1827025Certificate of AnalysisApr 26, 2022 N104910
H1827024Certificate of AnalysisApr 26, 2022 N104910
E2224127Certificate of AnalysisMar 19, 2022 N104910
E2224134Certificate of AnalysisMar 19, 2022 N104910

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Chemical and Physical Properties

SolubilitySoluble in water; Soluble in Ethanol,Methanol
SensitivityLight Sensitive,Air Sensitive,Heat Sensitive
Melt Point(°C)200°C

Safety and Hazards(GHS)

Pictogram(s) GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

H302:Harmful if swallowed

H312:Harmful in contact with skin

H332:Harmful if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P330:Rinse mouth.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P301+P317:IF SWALLOWED: Get medical help.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3
RTECS XF8045000
Reaxy-Rn 3896823

Related Documents

 SDS

 Specification Sheet

Alternative Products

  1. Nitro blue tetrazolium chloride (NBT)
    Nitro blue tetrazolium chloride (NBT)

    Starting at $197.90

  2. Nitrotetrazolium Blue chloride
    Nitrotetrazolium Blue chloride

    Starting at $11.90

References

1. Sircar G et al..  (2016)  Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES..  J Biol Chem,  291  (34): (18016-29).  [PMID:27358405]
2. Chhetri J et al..  (2022)  NQO1 protects against clioquinol toxicity..  Front Pharmacol,  13  (1000278).  [PMID:36267290]
3. Ukhueduan B et al..  (2022)  Relationship between oxidative stress and lifespan in Daphnia pulex..  Sci Rep,  12  (2354).  [PMID:35149730]

Solution Calculators

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