| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab087287-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab087287-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $198.90 | |
| Ab087287-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $309.90 | |
| Ab087287-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,319.90 |
pAb; Rabbit anti Human NOTCH1 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | NOTCH1 Antibody - Primary antibody, specific to NOTCH1, Rabbit IgG |
|---|---|
| Synonyms | hN1 antibody | Neurogenic locus Notch homolog protein 1 antibody | NICD antibody | NOTC1_HUMAN antibody | Notch 1 antibody | Notch 1 intracellular domain antibody | Notch homolog 1 translocation associated (Drosophila) antibody | notch1 antibody | TAN1 an |
| Specifications & Purity | ExactAb™, Validated, 1.5 mg/mL |
| Host species | Rabbit |
| Specificity | NOTCH1 |
| Immunogen | A synthetic peptide derived from human Notch1 (AA 2505-2519). |
| Positive Control | WB: MCF-7, Jurkat, HEK293 cell lysates; IHC: Human liver; IF: HeLa. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Rabbit anti Human NOTCH1 Antibody, Polyclonal antibody, could be used for WB, IHC, ICC, IF and so on. Application： WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function： Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Post-translational Post-translational: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose. Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
NOTCH1 Antibody (Ab087287) - Western Blot
All lanes: NOTCH1 Antibody (Ab087287) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 120 kDa
Observed band size: 112 kDa
Exposure time: 3.0 s
NOTCH1 Antibody (Ab087287) - IHC
Immunohistochemical analysis of paraffin-embedded Human liver using NOTCH1 Antibody (Ab087287). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
NOTCH1 Antibody (Ab087287) - ICC/IF
Immunofluorescent analysis of NOTCH1 in HeLa cells using NOTCH1 Antibody (Ab087287).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 "> 1/50 - 1/200 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0800794 | Certificate of Analysis | Aug 14, 2023 | Ab087287 |
| ZJ23F0800793 | Certificate of Analysis | Aug 14, 2023 | Ab087287 |
| 1. Nam Y, Sliz P, Song L, Aster JC, Blacklow SC. (2006) Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.. Cell, 124 (5): (973-83). [PMID:16530044] [10.1021/op500134e] |
| 2. Garg V, Muth AN, Ransom JF, Schluterman MK, Barnes R, King IN, Grossfeld PD, Srivastava D. (2005) Mutations in NOTCH1 cause aortic valve disease.. Nature, 437 (7056): (270-4). [PMID:16025100] [10.1021/op500134e] |
| 3. Stittrich AB, Lehman A, Bodian DL, Ashworth J, Zong Z, Li H, Lam P, Khromykh A, Iyer RK, Vockley JG et al.. (2014) Mutations in NOTCH1 cause Adams-Oliver syndrome.. Am J Hum Genet, 95 (3): (275-84). [PMID:25132448] [10.1021/op500134e] |
| 4. Astudillo L, Da Silva TG, Wang Z, Han X, Jin K, VanWye J, Zhu X, Weaver K, Oashi T, Lopes PE et al.. (2016) The Small Molecule IMR-1 Inhibits the Notch Transcriptional Activation Complex to Suppress Tumorigenesis.. Cancer Res, 76 (12): (3593-603). [PMID:27197169] [10.1021/op500134e] |
| 5. Flesch D, Ness J, Lamers C, Dehm F, Popella S, Steri R, Ogorek I, Hieke M, Dannhardt G, Werz O, Weggen S, Schubert-Zsilavecz M.. (2015) SAR-studies of γ-secretase modulators with PPARγ-agonistic and 5-lipoxygenase-inhibitory activity for Alzheimer's disease.. Bioorg Med Chem Lett, 25 (4): (841-846). [PMID:25575659] [10.1016/j.bmcl.2014.12.073] |
| 6. Shan W, Balog A, Quesnelle C, Gill P, Han WC, Norris D, Mandal S, Thiruvenkadam R, Gona KB, Thiyagarajan K, Kandula S, McGlinchey K, Menard K, Wen ML, Rose A, White R, Guarino V, Shen DR, Cvijic ME, Ranasinghe A, Dai J, Zhang Y, Wu DR, Mathur A, Rampulla R, Trainor G, Hunt JT, Vite GD, Westhouse R, Lee FY, Gavai AV.. (2015) BMS-871: a novel orally active pan-Notch inhibitor as an anticancer agent.. Bioorg Med Chem Lett, 25 (9): (1905-1909). [PMID:25857941] [10.1016/j.bmcl.2015.03.038] |
| 7. Yoneyama T, Arai MA, Akamine R, Koryudzu K, Tsuchiya A, Sadhu SK, Ahmed F, Itoh M, Okamoto R, Ishibashi M.. (2017) Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells.. J Nat Prod, 80 (9): (2453-2461). [PMID:28817274] [10.1021/acs.jnatprod.7b00282] |
| 8. Gómez-Galeno JE, Hurtado C, Cheng J, Yardimci C, Mercola M, Cashman JR.. (2018) b-Annulated 1,4-dihydropyridines as Notch inhibitors.. Bioorg Med Chem Lett, 28 (20): (3363-3367). [PMID:30201292] [10.1016/j.bmcl.2018.09.002] |
| 9. Quaglio D, Zhdanovskaya N, Tobajas G, Cuartas V, Balducci S, Christodoulou MS, Fabrizi G, Gargantilla M, Priego EM, Carmona Pestaña Á, Passarella D, Screpanti I, Botta B, Palermo R, Mori M, Ghirga F, Pérez-Pérez MJ.. (2019) Chalcones and Chalcone-mimetic Derivatives as Notch Inhibitors in a Model of T-cell Acute Lymphoblastic Leukemia.. ACS Med Chem Lett, 10 (4): (639-643). [PMID:30996810] [10.1021/acsmedchemlett.8b00608] |
| 10. Ellisen, L W LW and 6 more authors.. (1991) TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms.. Cell, (23): [PMID:1831692] |
| 11. Matsuno, K K and 7 more authors.. (1998) Human deltex is a conserved regulator of Notch signalling.. Nature genetics, [PMID:9590294] |
| 12. Gray, G E GE and 9 more authors.. (1999) Human ligands of the Notch receptor.. The American journal of pathology, [PMID:10079256] |
| 13. Zhou, S S and 6 more authors.. (2000) SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function.. Molecular and cellular biology, [PMID:10713164] |
| 14. Wu, L L and 5 more authors.. (2000) MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors.. Nature genetics, [PMID:11101851] |
| 15. Sakamoto, Kei K and 8 more authors.. (2002) The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.. The Journal of biological chemistry, (16): [PMID:12050162] |
| 16. Wu, Lizi L, Sun, Tao T, Kobayashi, Karla K, Gao, Ping P and Griffin, James D JD.. (2002) Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors.. Molecular and cellular biology, [PMID:12370315] |
| 17. Vardar, Didem D, North, Christopher L CL, Sanchez-Irizarry, Cheryll C, Aster, Jon C JC and Blacklow, Stephen C SC.. (2003) Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1.. Biochemistry, (17): [PMID:12795601] |
| 18. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053] |
| 19. Hambleton, Sophie S and 7 more authors.. (2004) Structural and functional properties of the human notch-1 ligand binding region.. Structure (London, England : 1993), [PMID:15576031] |
| 20. Ehebauer, Matthias T MT, Chirgadze, Dimitri Y DY, Hayward, Penny P, Martinez Arias, Alfonso A and Blundell, Tom L TL.. (2005) High-resolution crystal structure of the human Notch 1 ankyrin domain.. The Biochemical journal, (15): [PMID:16011479] |
| 21. Coleman, Mathew L ML and 12 more authors.. (2007) Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor.. The Journal of biological chemistry, (17): [PMID:17573339] |
| 22. Chastagner, Patricia P, Israël, Alain A and Brou, Christel C.. (2008) AIP4/Itch regulates Notch receptor degradation in the absence of ligand.. PloS one, (16): [PMID:18628966] |
| 23. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 24. Brütsch, René R and 8 more authors.. (2010) Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis.. Circulation research, (3): [PMID:20616313] |
| 25. Mo, Jung-Soon JS and 13 more authors.. (2011) Serum- and glucocorticoid-inducible kinase 1 (SGK1) controls Notch1 signaling by downregulation of protein stability through Fbw7 ubiquitin ligase.. Journal of cell science, (1): [PMID:21147854] |
| 26. Vasquez-Del Carpio, Rodrigo R and 6 more authors.. (2011) Assembly of a Notch transcriptional activation complex requires multimerization.. Molecular and cellular biology, [PMID:21245387] |
| 27. Gupta-Rossi, Neetu N and 6 more authors.. (2011) The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway.. The Journal of biological chemistry, (27): [PMID:21464124] |
| 28. Lim, Seung-Oe SO and 7 more authors.. (2011) Notch1 binds and induces degradation of Snail in hepatocellular carcinoma.. BMC biology, (30): [PMID:22128911] |
| 29. Puca, Loredana L, Chastagner, Patricia P, Meas-Yedid, Vannary V, Israël, Alain A and Brou, Christel C.. (2013) Α-arrestin 1 (ARRDC1) and β-arrestins cooperate to mediate Notch degradation in mammals.. Journal of cell science, (1): [PMID:23886940] |
| 30. Boskovski, Marko T MT and 7 more authors.. (2013) The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality.. Nature, (19): [PMID:24226769] |
| 31. Chakrabarty, Broto and Parekh, Nita.. (2014) Identifying tandem Ankyrin repeats in protein structures.. BMC bioinformatics, (30): [PMID:25547411] |
| 32. Pinnell, Nancy N and 14 more authors.. (2015) The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia.. Immunity, (17): [PMID:26522984] |
| 33. Deatherage, Catherine L CL and 7 more authors.. (2017) Structural and biochemical differences between the Notch and the amyloid precursor protein transmembrane domains.. Science advances, [PMID:28439555] |
| 34. Takeuchi, Hideyuki H and 6 more authors.. (2018) Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling.. Proceedings of the National Academy of Sciences of the United States of America, (4): [PMID:30127001] |
