mAb (C10); Mouse anti Human NSE Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab118776-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab118776-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $778.90 | |
| Ab118776-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $189.90 | |
| Ab118776-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $279.90 | |
| Ab118776-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (C10); Mouse anti Human NSE Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | NSE Mouse mAb - Primary antibody, specific to NSE, Mouse IgG2b |
|---|---|
| Synonyms | 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Lot by Lot |
| Host species | Mouse |
| Specificity | NSE |
| Immunogen | Recombinant NSE expressed in E.coli (AA 2-285) |
| Positive Control | WB: Hep G2, NIH/3T3, C6 and Jurkat cell lysates. IHC: Human Glioma Tissue, Human Cerebrum Tissue. IF/ICC: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human NSE Antibody, Monoclonal (C10), could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
NSE Mouse mAb (Ab118776) - Western Blot
All lanes: NSE Mouse mAb (Ab118776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 16.4 s
NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Glioma Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
NSE Mouse mAb (Ab118776) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 1µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.5-2 µg/mL |
| ICC/IF |
5-30 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901047 | Certificate of Analysis | Sep 18, 2023 | Ab118776 |
| ZJ23F0901046 | Certificate of Analysis | Sep 18, 2023 | Ab118776 |
| 1. He Huang,Shuang Tang,Ming Ji,Zhanyun Tang,Miho Shimada,Xiaojing Liu,Shankang Qi,Jason W Locasale,Robert G Roeder,Yingming Zhao,Xiaoling Li. (2018-05-19) p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis.. Molecular cell, 70 ((4)): (663-678). [PMID:29775581] |
| 2. Oliva, D D, Calì, L L, Feo, S S and Giallongo, A A.. (1991) Complete structure of the human gene encoding neuron-specific enolase.. Genomics, [PMID:2045099] |
| 3. Oliva, D D, Barba, G G, Barbieri, G G, Giallongo, A A and Feo, S S.. (1989) Cloning, expression and sequence homologies of cDNA for human gamma enolase.. Gene, (15): [PMID:2792767] |
| 4. McAleese, S M SM, Dunbar, B B, Fothergill, J E JE, Hinks, L J LJ and Day, I N IN.. (1988) Complete amino acid sequence of the neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE).. European journal of biochemistry, (15): [PMID:3208766] |
| 5. Van Obberghen, E E and 5 more authors.. (1988) Human gamma enolase: isolation of a cDNA clone and expression in normal and tumor tissues of human origin.. Journal of neuroscience research, [PMID:3385803] |
| 6. Day, I N IN, Allsopp, M T MT, Moore, D C DC and Thompson, R J RJ.. (1987) Sequence conservation in the 3'-untranslated regions of neurone-specific enolase, lymphokine and protooncogene mRNAs.. FEBS letters, (28): [PMID:3653393] |
| 7. Ansari-Lari, M A MA, Shen, Y Y, Muzny, D M DM, Lee, W W and Gibbs, R A RA.. (1997) Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.. Genome research, [PMID:9074930] |
| 8. Chai, Geqing G and 5 more authors.. (2004) Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase.. Journal of molecular biology, (20): [PMID:15289101] |
| 9. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 10. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075] |
| 11. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 12. Leonard, Paul G PG and 17 more authors.. (2016) SF2312 is a natural phosphonate inhibitor of enolase.. Nature chemical biology, [PMID:27723749] |
